Search results for "Isolation"
showing 10 items of 582 documents
Isolation and Culture of Oligodendrocytes
2019
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
Isolation and characterization of microsatellite markers in the liver fluke (Fasciola hepatica)
2004
PRIMER NOTE; International audience; Six microsatellite markers were isolated from Fasciola hepatica, a re-emerging parasite that causes important veterinary and public health problems. In a sample of 52 liver flukes from a region of hyperendemicity (Bolivian Altiplano), five microsatellite were polymorphic. Our results showed that liver flukes present important genetic variability, suggesting a preferential outcrossing reproduction mode for this hermaphroditic parasite.
Vibrational Spectrum of HXeSH revisited : Combined computational and experimental study
2020
Vibrational spectrum of HXeSH embedded in low-temperature matrix is experimentally studied. To support the spectrum interpretation, anharmonic vibrational analysis is performed using different models and basis sets and the data is compared with previous experimental and theoretical analyses. Computations of overtones and combination modes allowed for new band assignments. The HXeSH molecule exhibits high anharmonicity similarly as other molecules from the noble-gas hydride family. Comparison of the employed computational methods shows once again that the modelling of the noble-gas compounds faces theoretical challenges to yield quantitatively reliable results. peerReviewed
Ecologia e distribuzione di Chamaerops humilis L. (Arecaceae) nella Sicilia nord-orientale
2014
I settori costieri e collinari della Sicilia nord-orientale e delle Eolie sono stati esplorati ai fini della raccolta di informazioni aggiornate sulla distribuzione dei popolamenti di palma nana e sulle caratteristiche ecologiche delle stazioni che li ospitano. I risultati delle indagini di campo sono stati integrati dalla verifica dei toponimi locali legati ai nomi dialettali della specie. Si con- ferma la totale assenza della palma nana lungo l’intero settore costiero dei Nebrodi. Dei nove popolamenti accertati, quattro si trovano alle Eolie, due sul versante ionico e tre sul versante tir- renico dei Peloritani; uno di questi ultimi, localizzato su Capo Calavà, risulta inedito. La conco- …
Conformational-Dependent Photodissociation of Glycolic Acid in an Argon Matrix
2023
Ultraviolet-induced photodissociation and photo-isomerization of the three most stable conformers (SSC, GAC, and AAT) of glycolic acid are investigated in a low-temperature solid argon matrix using FTIR spectroscopy and employing laser radiation with wavelengths of 212 nm, 226 nm, and 230 nm. The present work broadens the wavelength range of photochemical studies of glycolic acid, thus extending the understanding of the overall photochemistry of the compound. The proposed kinetic model for the photodissociation of glycolic acid proceeds from the lowest energy conformer (SSC). The model suggests that ultraviolet light induces isomerization only between the SSC and GAC conformers and between …
UInDeSI4.0 : An efficient Unsupervised Intrusion Detection System for network traffic flow in Industry 4.0 ecosystem
2023
In an Industry 4.0 ecosystem, all the essential components are digitally interconnected, and automation is integrated for higher productivity. However, it invites the risk of increasing cyber-attacks amid the current cyber explosion. The identification and monitoring of these malicious cyber-attacks and intrusions need efficient threat intelligence techniques or intrusion detection systems (IDSs). Reducing the false positive rate in detecting cyber threats is an important step for a safer and reliable environment in any industrial ecosystem. Available approaches for intrusion detection often suffer from high computational costs due to large number of feature instances. Therefore, this paper…
Study of xT scaling in proton-proton collisions measured by the ALICE experiment
2012
In this Master's Thesis an analysis of proton proton data measured by the ALICE (A Large Ion Collider Experiment) Collaboration at center-of-mass energies 7 TeV and 2.76 TeV is presented. The ALICE detector is located at the LHC (Large Hadron Collider) at CERN, Switzerland. The goal of this thesis was to study whether the isolation requirement can increase the sensitivity of the xT spectra (xT=2pT/sqrt(s)) to higher-twist (HT) processes. In HT processes the outgoing hadron is produced directly in the hard subprocess, rather than in the fragmentation process described by the leading-twist (LT) processes in the perturbative QCD picture. Higher-twist processes are mainly low pT phenomena, caus…
Analysis of MAC-level throughput in LTE systems with link rate adaptation and HARQ protocols
2015
LTE is rapidly gaining momentum for building future 4G cellular systems, and real operational networks are under deployment worldwide. To achieve high throughput performance, in addition to an advanced physical layer design LTE exploits a combination of sophisticated mechanisms at the radio resource management layer. Clearly, this makes difficult to develop analytical tools to accurately assess and optimise the user perceived throughput under realistic channel assumptions. Thus, most existing studies focus only on link-layer throughput or consider individual mechanisms in isolation. The main contribution of this paper is a unified modelling framework of the MAC-level downlink throughput of …
Do allopatric male Calopteryx virgo damselflies learn species recognition?
2012
There is a growing amount of empirical evidence that premating reproductive isolation of two closely related species can be reinforced by natural selection arising from avoidance of maladaptive hybridization. However, as an alternative for this popular reinforcement theory, it has been suggested that learning to prefer conspecifics or to discriminate heterospecifics could cause a similar pattern of reinforced premating isolation, but this possibility is much less studied. Here, we report results of a field experiment in which we examined (i) whether allopatric Calopteryx virgo damselfly males that have not encountered heterospecific females of the congener C. splendens initially show discri…
Cannabis and Other Hallucinogenic Substance use and Abuse in the Context of COVID-19 Related Stressors
2022
Ievads: Kanabinoīdi ir pasaulē visvairāk lietotā narkotiskā viela.. PVO ģenerāldirektors 2020. gada 11. martā pasludināja COVID-19 pandēmiju. COVID-19 pandēmijas laikā daudzas valstis ieviesa ierobežojumus, lai ierobežotu jaunā koronavīrusa izplatīšanos, savukārt šie ierobežojumi var izraisīt garīgās veselības problēmas. Vairāki pētījumi liecina, ka COVID-19 izraisītā stresa pārvarēšanai tika lietota marihuāna vai citi halucinogēni. Mērķi: Tika sagatavots literatūras apskats, kurā apkopota jaunākā literatūra par vielu lietošanu pandēmijas laikā un kas ļauj salīdzināt to ar aptaujas datiem. Pētījuma, kas tika veikts no novembra līdz februārim, mērķis bija novērtēt riska un aizsargājošos fakt…