Search results for "Isolation"
showing 10 items of 582 documents
Biosurfactant production by hydrocarbon-degradingBrevibacteriumandVibrioisolates from the sea penPteroeides spinosum(Ellis, 1764)
2016
Among filter-feeders, pennatulids are the most complex and polymorphic members of the cnidarian class Anthozoa. They display a wide distribution throughout all the oceans, constituting a significant component of the sessile megafauna from intertidal to abyssal depths. In this study, a total of 118 bacterial isolates from enrichment cultures, carried out with homogenates of the sea pen Pteroeides spinosum (Ellis, 1764), were screened for hydrocarbon utilization by using the 2,6-dichlorophenol indophenol assay. Among them, 83 hydrocarbon-oxidizing isolates were analyzed for biosurfactant production by standard screening tests (i.e., emulsifying activity, E24 detection, surface tension measure…
Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation.
2019
ABSTRACT Physical activity initiates a wide range of multi-systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise-associated adaptive systemic signalling. Circulating EVs comprise a heterogeneous collection of different EV-subclasses released from various cell types. So far, a comprehensive picture of the parental and target cell types, EV-subpopulation diversity and functional properties of EVs released during exercise (ExerVs) is lacking. Here, we performed a detailed EV-phenotyping analysis to explore the cellular origin and potential …
A Method for Isolation of the Virome from Plasma Samples
2018
Virome studies are of special interest nowadays. Understanding viral communities in different body compartments will help guide future personalized treatments and to discern between homeostasis and disease. High-throughput sequencing technologies allow us to detect all the nucleic acids present in a sample, including viral ones, by random sequencing. One of the major challenges in virome studies is the correct isolation of the viral nucleic acids from a specific sample. This can be done during the extraction steps (e.g., enrichment of viral capsids), or during the bioinformatic analysis (e.g., removing all human and bacterial sequences). Furthermore, it is an important remark that the treat…
Primary Mucosal Epithelial Cell Cultivation: A Reliable and Accelerated Isolation
2019
We illustrate a reliable and accelerated isolation routine for mucosal epithelial cells, which thereupon can be used for soft tissue engineering. This is highly important in the field of soft tissue engineering because mucosal equivalents are frequently usable in several surgical fields like gynecology, urology, otorhinolaryngology, ophthalmology, maxillofacial surgery, and many others. In this context the isolation of mucosal epithelial cells suitable for tissue engineering is mandatory. The reliable cultivation of mucosal or skin epithelial cells is challenging and there is currently no reproducible method. We demonstrate a solution for this problem by developing an accelerated and nevert…
Prevalence and sequelae of self-reported and other-reported sexual abuse in adults with intellectual disability
2018
Background Sexual victimisation is an important problem that affects millions of people around the world, especially those with some kind of disability. The aim of this study was to determine the prevalence of self-reported and documented sexual abuse in people with mild or moderate intellectual disability and to analyse the sequelae that such experiences can have on their psychosocial health. Methods The sample consisted of 360 adults (50% men and 50% women) between 18 and 55 years of age (M = 39.87; standard deviation = 10.55). Results The prevalence of sexual abuse is 6.10% when it is self-reported (9.4% in women and 2.8% in men) and 28.6% when it is reported by professionals (27.8% in w…
The leftovers. The dead in life and social disappearance.
2020
Through an analysis of the TV series The Leftovers, we delve into the concept of "social disappearance" and into how it expresses the limits between life and death. The analysis focuses on the event that drives the plot: the mass disappearance of millions of people without reason. It has three moments: (1) the reconstruction of the order that the disappearance has broken; (2) the deviation of the mourning processes from their original logic; and (3) the acceptance that in the post-disappearance world nothing will be the same as before. The text offers some suggestions for thinking about possible lives in a world that is broken and with no promise of reconstruction, a world in which "social …
Managing stress during the coronavirus disease 2019 pandemic and beyond: Reappraisal and mindset approaches
2020
The novel coronavirus disease 2019 (COVID‐19) pandemic is a global public health crisis of a scale not previously experienced in modern times (Kickbusch et al., 2020). Governmental ‘lockdown’ measures aimed at minimizing virus transmission including ‘stay at home’ orders, closure of businesses and places of congregation, and travel restrictions have had a substantive societal impact that permeates almost every facet of daily life (Gostin & Wiley, 2020; Shanafelt, Ripp, & Trockel, 2020). These widespread changes represent considerable sources of stress in the population and will have deleterious effects on mental and physical health going forward. As nations begin to emerge from ‘lockdown’, …
Psychological countermeasures in manned space missions: “EARTH” system for the Mars-500 project
2016
Spaceflights missions have revealed that exist critical psychological problems. The Mars-500 project was an isolation and confinement experiment for identifying problems and solutions related to spaceflights missions. Medical countermeasures, such as physical exercise for 'bone degradation' are well recognized, but it is also important to develop psychological countermeasures for a possible 'psychological degradation'.Clinical Psychology has techniques useful for treating psychological problems, recent advances in Positive Psychology open up new possibilities for promoting resilience, and Information and Communication Technologies (ICT) have proven their utility for enhancing psychological …
Guidelines for the Direct Detection ofAnaplasmaspp. in Diagnosis and Epidemiological Studies
2017
The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, th…
The Dark Side of the Web—A Risk for Children and Adolescents Challenged by Isolation during the Novel Coronavirus 2019 Pandemic
2021
In response to the global novel coronavirus disease 2019 (COVID-19) pandemic, many countries around the world adopted social isolation measures to contain the spread of the virus.1 For children and adolescents, limitations in faceto-face activities and interactions with their traditional peer groups has been a frustrating experience. After disease containment measures, which included school closures, social distancing, and home quarantine, children and adolescents faced a prolonged state of physical isolation from their peers, teachers, extended family, and community networks that affects their emotional and behavioral health.2 Parents and pediatricians are reporting signs of mental distres…