Search results for "Italy"

showing 10 items of 2893 documents

Data mining from a 27-years rotavirus surveillance in Palermo, Italy.

2014

Uninterrupted surveillance conducted in Palermo, Sicily, for 27 years (1985–2012) detected rotavirus infection in 32.7% of 6522 children <5 years of age, hospitalised at the “G. Di Cristina” Children’s Hospital of Palermo. Increased rotavirus activity usually occurred from the beginning of winter to mid-spring. G1P[8] rotaviruses were the prevalent strains in most of the years and were only occasionally overcome by G9P[8], G4P[8] or G2P[4]. The circulation of non-G1P[8] strains was discontinuous and fluctuating. Phylogenetic analyses revealed an heterogeneous population of viruses within each genotype, with different lineages and sublineages emerging over the time. Amino acid substitutions …

Microbiology (medical)RotavirusGenotypingSettore MED/07 - Microbiologia E Microbiologia ClinicaSettore MED/17 - Malattie InfettiveGenotypeEpidemiologyBiologymedicine.disease_causeMicrobiologyRotavirus InfectionsRotavirusPhylogenetic analyseGeneticsmedicineData MiningHumansPublic Health SurveillanceMolecular BiologySicilyEcology Evolution Behavior and SystematicsPhylogenyRetrospective StudiesMolecular EpidemiologyInfant NewbornGenetic VariationInfantRotaviruVirologyRotavirus infectionInfectious DiseasesItalyChild PreschoolInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
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Salmonella bongori48:z35:– in Migratory Birds, Italy

2009

Suggested citation for this article: Foti M, Daidone A, Aleo A, Pizzimenti A, Giacopello C, Mammina C. Salmonella bongori 48:z35:– in migratory birds, Italy. Emerg Infect Dis [serial on the Internet]. 2009 Mar [date cited]. Available from http://www.cdc.gov/EID/content/15/3/502.htm

Microbiology (medical)Salmonella bongoriSerotypeSalmonellaEpidemiologyletterlcsh:MedicineSettore MED/42 - Igiene Generale E Applicatamedicine.disease_causeSalmonella bongorilcsh:Infectious and parasitic diseasesBirdsSalmonellaPrevalencemedicineAnimalslcsh:RC109-216Salmonella bongori; wild birds; PFGE; epidemiologySerotypingLetters to the Editorwild birdsBird DiseasesSalmonella Infections AnimalbiologyBird Diseaseslcsh:RPFGESalmonella bongori migratory birds epidemiologybiology.organism_classificationVirologyElectrophoresis Gel Pulsed-FieldInfectious DiseasesItalyAnimal MigrationEmerging Infectious Diseases
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Characterization of Listeria monocytogenes isolates from human listeriosis cases in Italy.

2009

ABSTRACT The objective of this study was to characterize by serotyping, pulsed-field gel electrophoresis (PFGE), and PCR amplification of virulence genes and markers of epidemic clones I, II, and III (ECI, ECII, and ECIII) 54 human isolates from apparently sporadic cases of infection occurring in the Lombardy region and in the province of Florence, Tuscany, Italy, in the years 1996 to 2007. Listeria monocytogenes isolates were provided by the clinical microbiology laboratories of the Lombardy region and the “Careggi” Hospital of Florence, Tuscany, Italy. Serotyping, PFGE after digestion with the AscI and ApaI enzymes, and PCR amplification for the inlA , inlC , and inlJ genes and ECI, ECII,…

Microbiology (medical)SerotypeGenotypeVirulence FactorsEpidemiologyepidemic clonesBiologySettore MED/42 - Igiene Generale E Applicatamedicine.disease_causePolymerase Chain Reactionlaw.inventionMicrobiologyListeria monocytogeneslawPregnancyGenotypehuman casemedicinePulsed-field gel electrophoresisCluster AnalysisHumansListeriosisSerotypingPolymerase chain reactionListeria monocytogeneAgedMolecular EpidemiologyMolecular epidemiologyInfant NewbornPFGEVirologyDNA FingerprintingListeria monocytogenesSubtypingBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldDNA profilingItalyFemaleJournal of clinical microbiology
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MLB1 Astrovirus in Children with Gastroenteritis, Italy

2014

To the Editor: Astroviruses are notable agents of gastroenteritis in many mammalian and avian hosts. Astroviruses are nonenveloped RNA small, round, viruses (SRVs) with a single-stranded, positive sense RNA of 6.1 to 7.9 kb (1). The genome contains 2 nonstructural genes, open reading frame (ORF) 1a and 1b, and a capsid gene, ORF2, with short 5′ and 3′ untranslated regions. Human astroviruses, a major cause of gastroenteritis, are classified in the human astrovirus species, comprising 8 serotypes (1). Recently, astroviruses genetically unrelated to canonical human astroviruses have been identified in human stools in several countries. These unusual astroviruses form 2 main genetic clades. On…

Microbiology (medical)SerotypeMaleLetterGenes ViralEpidemiologyvirusesMolecular Sequence Datalcsh:Medicinemedicine.disease_causeAstrovirusMicrobiologylcsh:Infectious and parasitic diseasesastrovirusfluids and secretionschildrenRotavirusMLB1 AstrovirusmedicineHumansviruseslcsh:RC109-216Letters to the EditorMLB1biologyenteric infectionslcsh:Rvirus diseasesInfantSapovirusbiology.organism_classificationmedicine.diseaseVirologyMolecular TypingDiarrheaInfectious DiseasesItalyChild PreschoolNorovirusEnterovirusAstroviridaeRNA Viralmedicine.symptomgastroenteritisEncephalitisEmerging Infectious Diseases
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Multidrug and broad-spectrum cephalosporin resistance among Salmonella enterica serotype enteritidis clinical isolates in southern Italy.

2002

ABSTRACT From 1992 to 1997, only six sporadic isolates of Salmonella enterica serotype Enteritidis from patients with cases of gastroenteritis in southern Italy exhibited resistance to broad-spectrum cephalosporins. Five isolates produced SHV-12, and one isolate encoded a class C β-lactamase. The bla SHV-12 gene was located in at least two different self-transferable plasmids, one of which also carried a novel class 1 integron.

Microbiology (medical)Serotypemedicine.drug_classEpidemiologySalmonella enteritidisCephalosporinIntegronbeta-LactamasesMicrobiologyPlasmidDrug Resistance Multiple BacterialGenotypemedicineHumansamoxicillin plus clavulanic acid; ampicillin; antibiotic agent; aztreonam; beta lactamase; cefotaxime; cefoxitin; ceftazidime; cephalosporin derivative; chloramphenicol; kanamycin; plasmid DNA; streptomycin; sulfonamide; tobramycin antibiotic resistance; article; bacterial infection; bacterium isolate; DNA probe; gastroenteritis; gastrointestinal infection; Italy; nonhuman; nucleotide sequence; phenotype; plasmid; priority journal; Salmonella; Salmonella enterica Base Sequence; beta-Lactamases; Cephalosporin Resistance; Cross Infection; Drug Resistance Multiple Bacterial; Gastroenteritis; Genes Bacterial; Humans; Italy; Plasmids; Salmonella enteritidis; Salmonella Infections Bacteria (microorganisms); Negibacteria; Salmonella; Salmonella entericaCephalosporin ResistanceCross InfectionbiologyBase SequenceCephalosporin Resistancebiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyGastroenteritisItalySalmonella enteritidisSalmonella entericaGenes BacterialSalmonella Infectionsbiology.proteinPlasmidsJournal of clinical microbiology
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Multiclonal emergence of carbapenem-resistant Klebsiella pneumoniae in Tuscany, Italy

2010

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaImipenemGenotypemedicine.drug_classCarbapenem resistant Klebsiella pneumoniaemedicine.medical_treatmentAntibioticsMicrobial Sensitivity TestsBiologySettore MED/42 - Igiene Generale E ApplicataMeropenembeta-Lactam Resistancebeta-LactamasesMicrobiologychemistry.chemical_compoundmedicineHumansPharmacology (medical)Molecular EpidemiologyKlebsiella pneumoniae resistenza ai carbapenemi multiclonaleGeneral MedicineDNA FingerprintingAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldKlebsiella InfectionsMultiple drug resistanceKlebsiella pneumoniaePhenotypeInfectious DiseasesCarbapenemsItalychemistryBeta-lactamaseErtapenemmedicine.drugBeta lactam antibioticsInternational Journal of Antimicrobial Agents
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Norovirus GII.4/Sydney/2012 in Italy, Winter 2012–2013

2013

To the Editor: Noroviruses (NoVs) are the major cause of acute gastroenteritis in children and adults; they are responsible for sporadic cases and outbreaks of gastroenteritis in various epidemiologic settings. NoVs can be classified genetically into at least 5 genogroups, GI to GV (1). Although >30 genotypes within genogroups GI, GII, and GIV can infect humans (2), a single genotype, GII.4, has been associated with most NoV-related outbreaks and sporadic cases of gastroenteritis worldwide (3). GII.4 NoV strains continuously undergo genetic/antigenic diversification and periodically generate novel strains through accumulation of punctate mutations or recombination. New GII.4 variants emerge…

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaLetterGenes ViralGenotypeEpidemiologySequence analysisviruseslcsh:MedicineBiologymedicine.disease_causeNorovirus GII.4 Italylcsh:Infectious and parasitic diseasesDisease Outbreaksfluids and secretionsGenotypemedicinePrevalencevariant Sydney 2012Humanslcsh:RC109-216virusesTypingviruses enteric diseasesLetters to the EditorCaliciviridae InfectionsIncidence (epidemiology)enteric infectionslcsh:RgenogroupsNorovirusvirus diseasesOutbreakVirologyGastroenteritisInfectious DiseasesCaliciviridae InfectionsItalyChild PreschoolNorovirussurveillanceMultilocus sequence typingSeasonsGII.4Multilocus Sequence TypingEmerging Infectious Diseases
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Lineage diversification and recombination in type-4 human astroviruses.

2013

Abstract Human astroviruses (HAstVs) are important enteric pathogens and can be classified genetically and antigenically into eight types. During surveillance of HAstVs in Italy, type-4 HAstVs were detected only sporadically and found to cluster into two distinct genetic groups. Upon sequence analysis of the 3′ end of the polymerase gene (ORF1b) and of the full-length ORF2, the 2008 type-4 HAstV strains were characterised as a novel ORF2 genetic lineage, designated as 4c. The 2008 type-4 HAstVs also shared the ORF1b gene with similar HAstV-4c strains detected globally, thus displaying a conserved ORF1b/ORF2 asset. By interrogation of the databases, this novel lineage 4c accounted for 60.8% …

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaLineage (genetic)Sequence analysisMolecular Sequence DataSequence alignmentBiologyMicrobiologyAstrovirusFecesOpen Reading FramesAstrovirus Epidemiology Genotyping Italy Viral gastroenteritisPhylogeneticsAstroviridae InfectionsGenetic variationGeneticsHumansAmino Acid SequenceMolecular BiologyGenotypingGeneEcology Evolution Behavior and SystematicsPhylogenyGeneticsRecombination GeneticBase SequenceSequence Homology Amino AcidSequence Analysis RNAvirus diseasesGenetic Variationbiology.organism_classificationRNA-Dependent RNA PolymeraseGastroenteritisInfectious DiseasesRNA ViralCapsid ProteinsSequence AlignmentMamastrovirusInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
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Characterization of Shiga toxin-producing Escherichia coli O157:H7 isolated in Italy and in France

2002

ABSTRACT Twenty-one Escherichia coli O157:H7 strains isolated in northern Italy from sporadic cases of hemolytic-uremic syndrome and from cattle and food were characterized by virulence gene analysis, pulsed-field gel electrophoresis (PFGE) of Xba I-digested DNA, enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR (ERIC-PCR), and antibiotic resistance patterns and compared to 18 strains isolated in France from human cases of diarrhea, cattle, and the environment. Strains isolated in Sicily (southern Italy) from a local farm (one strain) and from calves just imported from France (11 strains) and Spain (six strains) were also typed. Whereas the eae and hlyA genes were al…

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaMeatEpidemiologyCattle DiseasesVirulenceMicrobial Sensitivity TestsEscherichia coli O157Shiga Toxin 1medicine.disease_causePolymerase Chain ReactionShiga Toxin 2law.inventionMicrobiologychemistry.chemical_compoundIntergenic regionShiga-like toxinlawGenotypePulsed-field gel electrophoresismedicineAnimalsHumansChildEscherichia coliEscherichia coli InfectionsPhylogenyPolymerase chain reactionVirulencebiologyShiga toxinShiga Toxin Escherichia coli O157:H7Anti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldItalychemistryHemolytic-Uremic Syndromebiology.proteinCattleFrance
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Surveillance of human astrovirus circulation in Italy 2002-2005: emergence of lineage 2c strains.

2010

AbstractBy screening faecal samples collected over four consecutive years (2002-2005) from hospitalized children with diarrhoea in Palermo, Italy, astroviruses (HAstVs) were detected in 3.95% of the patients. The predominant type circulating was HAstV-1 but, in 2002, only HAstV-2 and -4 were identified. Interestingly, the HAstVs-2 detected appeared to be consistently different in 5′ end of their open reading frame 2 from the previously described subtypes. These novel type 2 strains were included in a new 2c lineage based on the phylogenetic analysis and the presence of nine peculiar substitutions.

Microbiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaPathologymedicine.medical_specialtyLineage (genetic)Settore MED/17 - Malattie Infettivesequence analysisSequence analysisMolecular Sequence DataPolymorphism Single NucleotideVirusAstrovirusAstrovirusFecesAstroviridae InfectionsGenotypePrevalenceMedicineHumansTypingChildGenotypingPhylogenyMolecular EpidemiologybiologyPhylogenetic treebusiness.industryGeneral Medicinebiology.organism_classificationVirologyAstrovirus gastroenteritis genotyping Italy sequence analysisInfectious DiseasesgenotypingItalyPopulation SurveillanceRNA ViralbusinessgastroenteritisMamastrovirusClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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