Search results for "Italy"

showing 10 items of 2893 documents

Molecular Characterization of Coxsackievirus B5 Isolates from Sewage, Italy 2016–2017

2019

Hereby, the partial Viral Protein 1 sequences of Coxsackievirus B5 (CV-B5) from sewage samples, collected in Italy from 2016 to 2017, were compared with those available in GenBank from clinical samples. Phylogenetic analysis highlighted: (I) the predominant circulation of CV-B5 genogroup B in Italy, and (II) the presence of two new sub-genogroups.

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaEpidemiologyViral proteinHealth Toxicology and Mutagenesisviruses030106 microbiologySewage010501 environmental sciencesBiologyCoxsackievirusmedicine.disease_causeBrief Communication01 natural sciences03 medical and health sciencesViral ProteinsPhylogenetic analysiNon-polio enterovirusePhylogeneticsVirologymedicineCoxsackievirusPhylogeny0105 earth and related environmental sciencesPoliovirusePhylogenetic analysisCV-B5Phylogenetic treeSewagebusiness.industryvirus diseasesNon-polio enterovirusesbiology.organism_classificationVirologyEnterovirus B HumanItalyGenBankPoliovirusesCoxsackievirubusinessFood ScienceEnvironmental Monitoring
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Norovirus GII.17 as Major Epidemic Strain in Italy, Winter 2015–16

2017

In winter 2015-16, norovirus GII.17 Kawasaki 2014 emerged as a cause of sporadic gastroenteritis in children in Italy. Median patient age was higher for those with GII.17 than GII.4 infection (55 vs. 24 months), suggesting limited cross-protection for older children.

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaEpidemiologyviruseslcsh:Medicinemedicine.disease_causeDisease Outbreaksfluids and secretionsEpidemiologyChildEpidemic strainCaliciviridae InfectionsNorovirus GIIvirus diseasesInfectious DiseasesItalyChild PreschoolPopulation SurveillanceSeasonsgastroenteritigastroenteritisNorovirus GII.17 as Major Epidemic Strain in Italy Winter 2015–16Microbiology (medical)medicine.medical_specialtyAdolescentGenotype030106 microbiologyenteric infectionHistory 21st Centurylcsh:Infectious and parasitic diseasesOpen Reading Frames03 medical and health sciencesPatient ageResearch LettermedicineHumansviruseslcsh:RC109-216Noroviruvirusebusiness.industryenteric infectionsNoroviruslcsh:RInfant NewbornInfantGII.17 Kawasaki 2014Virology030104 developmental biologyNorovirusbusinessEmerging Infectious Diseases
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Temporal variation in the distribution of type-1 human astrovirus lineages in a settled population over 14 years.

2016

Human astroviruses (HAstVs) are important enteric pathogens that are genetically and antigenically heterogeneous and can be classified into eight sero/genotypes (HAstV-1 to -8) and different lineages within each HAstV type. This study describes the genetic diversity of HAstVs circulating in southern Italy over 14 years. Molecular analysis of HAstV-1 strains showed that three different lineages (1a, 1b and 1d) of the predominant genotype were circulating during the study period. The study of an archival collection of HAstV strains offers a unique opportunity to evaluate the patterns of variation of HAstV infections over the years and to correlate the observed epidemiological changes to the g…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinicamedicine.medical_specialtyTime FactorsGenotypePopulationGenome ViralBiology03 medical and health sciencesOpen Reading FramesMedical microbiologyVirologyAstroviridae InfectionsAstrovirus genotyping ItalyGenotypemedicineHumansGenetic variabilityeducationPhylogenyGeneticseducation.field_of_studyGenetic diversityMolecular EpidemiologyGenetic VariationGeneral MedicineHuman astrovirusMolecular analysis030104 developmental biologyItalyMamastrovirusArchives of virology
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Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone

2017

ABSTRACT : The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and t…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinicasiderophoreantibiotic resistancelong term survivalsequence analysisKlebsiella pneumoniaepolymerase chain reactionResponses to Human InterventionsDrug ResistanceGene TransferClone (cell biology)ST259bacterial proteinvirulence factorYersiniabactinGenomechemistry.chemical_compoundMicrobialPlasmidAntibioticsbacterial genomepathogenicitygenetics610 Medicine & healthgenome analysisCross InfectionMolecular EpidemiologyGenomeVirulencebiologydrug effectyersiniabactinBacterialDrug Resistance MicrobialGeneral MedicineKlebsiella infectionglycogen synthesisKlebsiella pneumoniaeEnglandItalyST307horizontal gene transferProteínas BacterianasResearch ArticleGene Transfer HorizontalVirulence FactorsSequence analysiscapsule030106 microbiologyVirulence610 Medicine & healthpulsed field gel electrophoresisColombiaCarbapenemase; siderophore; yersiniabactin; bacterial protein; beta lactamase; virulence factor antibiotic resistance; Article; bacterial strain; bacterial virulence; bacterium isolate; fimbria; genome analysis; glycogen synthesis; Klebsiella pneumoniae; long term survival; microbial diversity; nonhuman; plasmid; polymerase chain reaction; pulsed field gel electrophoresis; sequence analysis; whole genome sequencing; antibiotic resistance; bacterial genome; carbapenem-resistant Enterobacteriaceae; Colombia; cross infection; drug effect; England; genetic variation; genetics; horizontal gene transfer; human; Italy; Klebsiella infection; microbiology; molecular epidemiology; multilocus sequence typing; pathogenicity; virulence Bacterial Proteins; beta-Lactamases; Carbapenem-Resistant Enterobacteriaceae; Colombia; Cross Infection; Drug Resistance Microbial; England; Gene Transfer Horizontal; Genetic Variation; Genome Bacterial; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Virulence; Virulence Factors; Whole Genome SequencingArticlebeta-Lactamasesbeta lactamaseHorizontalMicrobiologyCarbapenemase03 medical and health sciencesAntibiotic resistanceBacterial ProteinsplasmidHumanshumanInfecciones por KlebsiellafimbrianonhumanWhole Genome Sequencingbacterial virulencebacterium isolatemicrobiologyGenetic Variationbacterial strainbiology.organism_classificationKlebsiella InfectionsEnterobacteriaceae Resistentes a los CarbapenémicosKPCCarbapenem-Resistant Enterobacteriaceae030104 developmental biologychemistrymicrobial diversityEpidemiología MolecularGenome BacterialWGSMultilocus Sequence Typing
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Clinical Characteristics and Outcomes of Patients with COVID-19 Infection: The Results of the SARS-RAS Study of the Italian Society of Hypertension

2021

: The COVID-19 infection has rapidly spread around the world and a second wave is sweeping in many countries. Different clinical and epidemiological aspects characterize the disease and their understanding is necessary to better face the management of the pandemic in progress. The Italian society of arterial hypertension with the SARS-RAS study has contributed significantly to the knowledge of the interaction between inhibition of the renin-angiotensin system and COVID-19 infection. Furthermore, the study results help to understand some of the main aspects related to mortality and morbidity deriving from the infection through a multicentre analysis throughout the national territory.

0301 basic medicineSettore MED/09 - Medicina InternaCross-sectional studyDiseaseComorbiditySeverity of Illness IndexComorbiditiesRenin-Angiotensin System0302 clinical medicineRisk FactorsSex-differences.EpidemiologyPandemicFrailtySars-Cov2Treatment OutcomeItalyAnti-hypertensive therapy; comorbidities; frailty; Sars-Cov2; sex-differencesHypertensionComorbiditiePosition PaperRisk assessmentCardiology and Cardiovascular Medicinemedicine.medical_specialtySex-differenceRisk Assessment03 medical and health sciencesPharmacotherapySex FactorsSeverity of illnessmedicineAnti-hypertensive therapy Sars-Cov2 Frailty Comorbidities Sex-differencesInternal MedicineHumansArterial PressureAnti-hypertensive therapy Comorbidities Frailty Sars-Cov2 Sex-differencesIntensive care medicineAnti-hypertensive therapyAntihypertensive AgentsAnti-hypertensive therapy; Comorbidities; Frailty; Sars-Cov2; Sex-differences; Antihypertensive Agents; Arterial Pressure; COVID-19; Comorbidity; Cross-Sectional Studies; Frailty; Humans; Hypertension; Italy; Renin-Angiotensin System; Risk Assessment; Risk Factors; Severity of Illness Index; Sex Factors; Treatment OutcomeSettore MED/14 - Nefrologiabusiness.industryCOVID-19medicine.diseaseComorbiditySex-differences030104 developmental biologyCross-Sectional Studiesbusiness030217 neurology & neurosurgery
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Daclatasvir-based regimens in HCV cirrhosis: experience from the Italian early access program

2019

AbstractWe reported the efficacy and safety data for daclatasvir (DCV)-based all-oral antiviral therapy in patients treated in the Italian compassionate-use program. 275 patients were included (202 male-73.5%, mean age: 57.4 years, 62 HIV-coinfected, 94 with recurrence of hepatitis C post-OLT). Forty-nine patients (17.8%) had Child-Pugh B, Genotype(G) distribution was: G1a:72 patients (26.2%), G1b:137 (49.8%); G3:40 (14.5%) and G4:26 (9.5%). Patients received DCV with sofosbuvir(SOF) (n = 221, 129 with ribavirin(RBV) or with simeprevir (SMV) or asunaprevir (ASU) (n = 54, 19 with RBV) for up to 24 weeks. Logistic regression was used to identify baseline characteristics associated with sustai…

0301 basic medicineSimeprevirLiver CirrhosisMalePyrrolidinesSofosbuvirSustained Virologic Responselcsh:MedicineSettore MED/05Gastroenterologychemistry.chemical_compound0302 clinical medicineLiver Function TestsINFECTIONMedicinePLUS SOFOSBUVIRlcsh:ScienceSulfonamidesMultidisciplinaryImidazolesValineHepatitis CMiddle AgedTreatment OutcomeItalySAFETYHCVSUSTAINED VIROLOGICAL RESPONSEDrug Therapy CombinationFemaleRIBAVIRINSettore BIO/19 - MICROBIOLOGIA GENERALECHRONIC HEPATITIS-Cmedicine.drugAdultmedicine.medical_specialtyDaclatasvirDrug-Related Side Effects and Adverse ReactionsAntiviral AgentsArticle03 medical and health sciencesInternal medicineHumansAgedADVANCED LIVER-DISEASEbusiness.industryRibavirinVIRUS GENOTYPE 3lcsh:RHepatitis C ChronicHCV HIV Daclatasvirmedicine.diseaseIsoquinolinesEFFICACYRegimen030104 developmental biologychemistryAsunaprevirlcsh:QLiver functionCarbamatesSofosbuvirbusiness030217 neurology & neurosurgeryScientific Reports
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Genomic inbreeding estimation in small populations: evaluation of runs of homozygosity in three local dairy cattle breeds

2016

In the local breeds with small population size, one of the most important problems is the increase of inbreeding coefficient (F). High levels of inbreeding lead to reduced genetic diversity and inbreeding depression. The availability of high-density single nucleotide polymorphism (SNP) arrays has facilitated the quantification of F by genomic markers in farm animals. Runs of homozygosity (ROH) are contiguous lengths of homozygous genotypes and represent an estimate of the degree of autozygosity at genome-wide level. The current study aims to quantify the genomic F derived from ROH (F-ROH) in three local dairy cattle breeds. F-ROH values were compared with F estimated from the genomic relati…

0301 basic medicineSingle-nucleotide polymorphismRuns of HomozygosityBiologyPolymorphism Single NucleotideSF1-1100local cattle breedSettore AGR/17 - Zootecnica Generale E Miglioramento Genetico03 medical and health sciencesAnimal sciencegenomic inbreeding; local cattle breeds; runs of homozygosity; Animal Science and ZoologyGenetic variationInbreeding depressionAnimalsInbreedinglocal cattle breedsDairy cattleGeneticsGenetic diversityruns of homozygositygenomic inbreedingHomozygote0402 animal and dairy scienceGenetic Variation04 agricultural and veterinary sciences040201 dairy & animal scienceBreedAnimal culture030104 developmental biologyItalyCattleFemaleAnimal Science and ZoologyInbreedingAnimal
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The Italian law on body donation: A position paper of the Italian College of Anatomists

2021

In Italy, recent legislation (Law No. 10/2020) has tuned regulations concerning the donation of one's postmortem body and tissues for study, training, and scientific research purposes. This study discusses several specific issues to optimise the applicability and effectiveness of such an important, novel regulatory setting. Some of these unsolved issues may involve the grantees of teaching and training activities, the role of academic anatomical institutes, the role of family members in the donation process, the universal time limit indicated for any donation, the handling of corpses, and the limited body donation and its subordination to the donation of organs and tissues. Critical issues …

0301 basic medicineStudents MedicalTissue and Organ ProcurementeducationTissue DonorLegislationBody donation programme Law Regulation Cadaver lab EducationCadaver labNOEducation03 medical and health sciencesBody donationAnatomistBody donation programme Law Regulation Cadaver lab EducationPolitical scienceMedicalCadaverHumansAnatomistsStudentsImplementationSettore BIO/16Body donation programmeSettore BIO/16 - Anatomia UmanaGeneral MedicineTissue DonorsTransplantationSubordination (finance)030104 developmental biologyItalyLawDonationBody donation programme; Cadaver lab; Education; Law; Regulation; Cadaver; Humans; Italy; Tissue Donors; Anatomists; Students Medical; Tissue and Organ ProcurementBody donation programme; Cadaver lab; Education; Law; RegulationPosition paperBody donation programme; Law; Regulation; Cadaver lab; Education030101 anatomy & morphologyAnatomyInefficiencyLawDevelopmental BiologyHumanRegulation
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Evaluation of antimicrobial resistance and virulence of enterococci from equipment surfaces, raw materials and traditional cheeses

2016

Forty enterococci isolated along the production chains of three traditional cheeses (PDO Pecorino Siciliano, PDO Vastedda della Valle del Belìce, and Caciocavallo Palermitano) made in Sicily (southern Italy) were studied for the assessment of their antibiotic resistance and virulence by a combined phenotypic/genotypic approach. A total of 31 Enterococcus displayed resistance to at least one or more of the antimicrobials tested. The strains exhibited high percentages of resistance to erythromycin (52.5%), ciprofloxacin (35.0%), quinupristin–dalfopristin (20.0%), tetracycline (17.5%), and high-level streptomycin (5.0%). The presence of tet(M), cat(pC221), and aadE genes for resistance to tetr…

0301 basic medicineTetracyclineFood HandlingVirulence Factors030106 microbiologyVirulenceMicrobial Sensitivity TestsAntimicrobial resistanceMicrobiologyMicrobiology03 medical and health sciencesAntibiotic resistanceBacterial ProteinsCheeseDrug Resistance BacterialmedicineAnimal rennetAnimal rennet Antimicrobial resistance Enterococcus Raw milk Traditional cheese Virulence Wooden vatAnimalsHumansGram-Positive Bacterial InfectionsbiologyVirulenceRaw milkTraditional cheeseAnimal rennet Antimicrobial resistance Enterococcus Raw milk Traditional cheese Virulence Wooden vatGeneral MedicineRaw milkbiochemical phenomena metabolism and nutritionbiology.organism_classificationAntimicrobialWooden vatAnti-Bacterial AgentsErythromycinCiprofloxacin030104 developmental biologyMilkEnterococcusEquipment and SuppliesItalyStreptomycinEquipment ContaminationCattleEnterococcusFood Sciencemedicine.drugSettore AGR/16 - Microbiologia Agraria
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Development of a method for the direct fermentation of semolina by selected sourdough lactic acid bacteria

2016

Three obligately heterofermentative lactic acid bacteria (LAB) strains (Lactobacillus sanfranciscensis PON100336, Leuconostoc citreum PON10079 and Weissella cibaria PON10030) were used in this study as a multi-species starter culture for sourdough production. The starter inoculum was prepared and propagated in sterile semolina extract (SSE) broth. Acidification kinetics, microbiological counts detected on specific media for sourdough LAB, polymorphic profile comparison and species-specific PCRs evidenced a stability of the liquid inoculum over time determining its suitability for direct addition to semolina. In order to validate this innovative method for the production of durum wheat (Trit…

0301 basic medicineWeissellaFlour030106 microbiologyLactobacillus sanfranciscensisFermentation; Lactic acid bacteria; Quality parameters; Sourdough; Starter culture; Triticum durum genotypes; Volatile organic compounds; Microbiology; Food Science; Safety Risk Reliability and Qualitymedicine.disease_causePolymerase Chain ReactionMicrobiology03 medical and health sciencesStarterLeuconostoc citreumLactobacillusLactic acid bacteriamedicineLeuconostocLactic AcidFood scienceWeissella cibariaSafety Risk Reliability and QualityTriticumbiologyChemistryfood and beveragesBreadSettore AGR/15 - Scienze E Tecnologie AlimentariGeneral MedicineVolatile organic compoundbiology.organism_classificationTriticum durum genotypeSettore AGR/02 - Agronomia E Coltivazioni ErbaceeQuality parameterLactobacillusStarter culture030104 developmental biologyItalyWeissellaSourdoughFermentationFermentationLeuconostocSettore AGR/16 - Microbiologia AgrariaFood ScienceInternational Journal of Food Microbiology
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