Search results for "KF"

Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range

2017

Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Here, we performed an independent comparison between single-pot solid-phase-enhanced sample preparation (SP3), filter-aided sample preparation (FASP), and a commercial kit based on the in-StageTip (iST) method. We assessed their performance for the processing of proteomic samples in the low μg range using varying amounts of HeLa cell lysate (1-20 μg of total protein). All three workflows showed similar performances for 20 μg of starting material. When handling sample sizes below 10 μg, the number of identified proteins and peptides as well as the quantitat…

A Proposed Knowledge Based Approach for Solving Proteomics Issues

2010

In this paper we present a novel knowledge-based approach that aims at helping scientists to face and resolve a large number of proteomics problem. The system architecture is based on an ontology to model the knowledge base, a reasoner that starting from the user's request and a set of rules builds the workflow of tasks to be done, and an executor that runs the algorithms and software scheduled by the reasoner. The system can interact with the user showing him intermediate results and several options in order to refine the workflow and supporting him to choose among different forks. Thanks to the presence of the knowledge base and the modularity provided by the ontology, the system can be e…

GeneTonic: an R/Bioconductor package for streamlining the interpretation of RNA-seq data

2021

AbstractBackgroundThe interpretation of results from transcriptome profiling experiments via RNA sequencing (RNA-seq) can be a complex task, where the essential information is distributed among different tabular and list formats - normalized expression values, results from differential expression analysis, and results from functional enrichment analyses. A number of tools and databases are widely used for the purpose of identification of relevant functional patterns, yet often their contextualization within the data and results at hand is not straightforward, especially if these analytic components are not combined together efficiently.ResultsWe developed the GeneTonic software package, whi…

Participants' perceptions of an intervention implemented in an Action Research Nursing Documentation Project.

2017

Aims and objectives The aim of this study is to describe healthcare professionals’ experiences and perceptions of an intervention implemented in an action research project conducted to improve nursing documentation practices in four municipalities in Norway. Background Documentation of individualized patient care is a continuing concern in healthcare services and could impacts the quality and safety of healthcare. Use of electronic systems has made some aspects of documentation more comprehensive, but creation of an individualized care plan remains a pressing issue. Design A qualitative descriptive design was used. Methods An action research project was conducted between 2010–2012 to improv…

Quantum chemical meta-workflows in MoSGrid

2014

Quantum chemical workflows can be built up within the science gateway Molecular Simulation Grid. Complex workflows required by the end users are dissected into smaller workflows that can be combined freely to larger meta-workflows. General quantum chemical workflows are described here as well as the real use case of a spectroscopic analysis resulting in an end-user desired meta-workflow. All workflow features are implemented via Web Services Parallel Grid Runtime and Developer Environment and submitted to UNICORE. The workflows are stored in the Molecular Simulation Grid repository and ported to the SHIWA repository. © 2014 John Wiley & Sons, Ltd.

Atgriezeniskā skaņas kanāla un ieraksta režīma testu automatizācija Axia Fusion pultīm

2017

Kvalifikācijas darbs ir izstrādāts, lai pārbaudītu vienas no "Telos Alliance" produkta, Axia Fusion Consoles, pielāgotajām un noklusētajām atpakaļejošajām opcijām un ieraksta režīmu opcijām. Pārbaudes process ietver atbilstošu konfigurāciju un iestatījumu iestatīšanu katram testa scenārijam un audio līmeņu pārbaudei vai vērtību iegūšanai, lai noskaidrotu, vai rezultāts atbilst sagaidāmam guvumam. Backfeed Test ir sadalīts divos dažādos moduļos: Default Backfeed tests un Custom Backfeed tests. Katrs modulis tiek tālāk iedalīts trīs dažādos moduļos; Konfigurācija, funkcionālie un testēšanas moduļi, kas atbilst to funkcijām un neatkarībai, kā arī galvenais modulis, kas organizē visus pārējos m…

The Cultural Roots of Risk

2017

This paper discusses the story of Mary and Roger an elderly couple who opted to buy a car, but later were pressed to sell it because they were unable to absorb the costs of insurance. The world of risks does not work in the same lines in North than South. This pieces invites the discussion to what extent risk perception is not an instrument for controlling workforce in under-developing nations. The mythologies of mobility have been formulated around life in the First World. They work as ideological devices to activate certain circuits of the economy, which in other times were relegated to peripheral positions. The industries of tourism and air travel transport millions travelers who enjoy t…

Slope instability in a historical and architectural interest site: the Agrigento hill (Sicily-Italy)

2014

Next-Generation Sequencing in Clinical Practice

2019

Abstract During the past few decades, Sanger sequencing represented the “gold standard” technique. In order to better define the mutational status of several genes at the same time, next-generation sequencing methodologies have been introduced in the molecular laboratory workflow. In the era of personalized medicine, this technological improvement plays a key role in the comprehensive molecular characterization of cancer patients in order to get a “tile” target therapy. For different cancer patients, different target therapies are available and different genes serve as clinical it relevant biomarkers. This chapter reviews the principal features of these novel technologies and their applicat…

Checkpointing Workflows for Fail-Stop Errors

2017

International audience; We consider the problem of orchestrating the exe- cution of workflow applications structured as Directed Acyclic Graphs (DAGs) on parallel computing platforms that are subject to fail-stop failures. The objective is to minimize expected overall execution time, or makespan. A solution to this problem consists of a schedule of the workflow tasks on the available processors and of a decision of which application data to checkpoint to stable storage, so as to mitigate the impact of processor failures. For general DAGs this problem is hopelessly intractable. In fact, given a solution, computing its expected makespan is still a difficult problem. To address this challenge,…