Search results for "Kali"
showing 10 items of 823 documents
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro
2021
The aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (BGLAP), periostin (POSTN), osteopontin (OPN), osterix (OSX), collagen type I alpha 1 chain (COL1A1), dentin matrix acidic phosphoprotein (DMP)1, RUNX family transcription factor (RUNX)2, and marker of proliferation Ki-67 (KI67) was quantified by real-time PCR. Protein expression was analyzed by immunocytochemistry and ELISA. ALP activity was determined by ALP assay. Von Kossa and alizarin r…
Amorphous polyphosphate–hydroxyapatite: A morphogenetically active substrate for bone-related SaOS-2 cells in vitro
2015
There is increasing evidence that inorganic calcium-polyphosphates (polyP) are involved in human bone hydroxyapatite (HA) formation. Here we investigated the morphology of the particles, containing calcium phosphate (CaP) with different concentrations of various Na-polyP concentrations, as well as their effects in cell culture. We used both SaOS-2 cells and human mesenchymal stem cells. The polymeric phosphate readily binds calcium ions under formation of insoluble precipitates. We found that addition of low concentrations of polyP (10wt.%, referred to the CaP deposits) results in an increased size of the HA crystals. Surprisingly, at higher polyP concentrations (10wt.%) the formation of cr…
The morphogenetically active polymer, inorganic polyphosphate complexed with GdCl 3 , as an inducer of hydroxyapatite formation in vitro
2015
Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the one caused by polyP (as calcium salt) and by GdCl3 alone, regarding their potencies to induce hydroxyapatite (HA) formation in SaOS-2 cells in vitro. The three compounds, GdCl3, polyP and polyP·Gd were found to be non-toxic at concentrations up to at least 30μM. Selecting a low, 5μM, concentration it was found that polyP·Gd significantly induced HA formation, as determined by Alizarin Red S staining and by quantit…
Effect of total sonicated Aggregatibacter actinomycetemcomitans fragments on gingival stem/progenitor cells
2018
Background Aggregatibacter-actinomycetemcomitans (A.actinomycetemcomitans) are strongly associated with localized-aggressive-periodontitis (LAgP). The study’s aim was to test for the first time the effect of total sonicated A.actinomycetemcomitans-bacterial-fragments on gingival mesenchymal stem/progenitor cells’ (G-MSCs) proliferation and regenerative gene expression in-vitro. Material and Methods G-MSCs were isolated, characterized, expanded and stimulated by total sonicated A.actinomycetemcomitans-bacterial-fragments (0 (negative-control), 15, 60, 120 and 240µg/ml; serovar-b; n=6/group). Cellular proliferation and NF-κβ (NFKB1), Alkaline Phosphatase (ALPL), Collagen-I (COL1A1), Collagen-…
Proteolytic Enzymes Clustered in Specialized Plasma-Membrane Domains Drive Endothelial Cells’ Migration
2016
In vitro cultured endothelial cells forming a continuous monolayer establish stable cell-cell contacts and acquire a "resting" phenotype; on the other hand, when growing in sparse conditions these cells acquire a migratory phenotype and invade the empty area of the culture. Culturing cells in different conditions, we compared expression and clustering of proteolytic enzymes in cells having migratory versus stationary behavior. In order to observe resting and migrating cells in the same microscopic field, a continuous cell monolayer was wounded. Increased expression of proteolytic enzymes was evident in cell membranes of migrating cells especially at sprouting sites and in shed membrane vesi…
European eel (<i>Anguilla anguilla</i>) plasma biochemistry alerts about propanil stress
2017
Propanil stress response in the eel (Anguilla anguilla) was examined. Eels were exposed to 3.16 mg/L for 72 hr and allowed to recover for 96 hr. Plasma levels of cortisol, lactate dehydrogenase (LDH), alkaline phosphatase (AP), aspartate aminotransferase (AST), cholesterol, triglycerides, glucose, ammonium, lactate, albumin, and total proteins as well as electrolytes (chloride, sodium, potassium, calcium and phosphorus) were determined. As a consequence of exposure, cortisol, AP, AST, and LDH increased. A hyperglycemic condition, together with hyperlactemia, hypoalbuminemia, hypoproteinemia, hypercholesterolemia and hypertriglycemia was registered. Ammonium increased during exposure concomi…
Specific inflammatory response of Anemonia sulcata (Cnidaria) after bacterial injection causes tissue reaction and enzymatic activity alteration
2015
The evolution of multicellular organisms was marked by adaptations to protect against pathogens. The mechanisms for discriminating the ’’self’’ from ’’non-self” have evolved into a long history of cellular and molecular strategies, from damage repair to the co-evolution of host-pathogen interactions. We investigated the inflammatory response in Anemonia sulcata (Cnidaria: Anthozoa) following injection of substances that varied in type and dimension, and observed clear, strong and specific reactions, especially after injection of Escherichia coli and Vibrio alginolyticus. Moreover, we analyzed enzymatic activity of protease, phosphatase and esterase, showing how the injection of different ba…
Denboratik kausara: -nez gero kausazko lokailuaren garapenaz
2019
LABURPENA Lan honetan -nez gero atzizki multzoaren garapena dugu aztergai, lehen testuetatik hasi eta gaurdaino. Denbora lokailuetatik sortu diren beste kausazko lokailu asko bezala, -nez gero batez ere mintza-ekintzak eta antzekoak justifikatzeko erabiltzen da. Hala ere, kausa neutroagoak adierazteko ere erabiltzen da. Gaztelaniaz ez bezala, menpeko perpausaren kokagunea ez da aldatu azken mendeotan eta funtzio informatiboan ere ez dugu aldakuntza handirik ikusten. Sumatzen dugun garapen nagusia da beharbada edukizko kausazko neutroetan maizago erabiltzeko joera. Oro har, -nez gero kausazko menderagailuak, gazt. ya que-rekin baino, antz handiagoa du fr. puisque eta ing. since lokailuekin. …
Radical sex role ideology and the Finnish gender role movement in the late 1960s
2022
An active discussion of gender roles and the need to renegotiate them took place in the late 1960s in Finland. While previous studies have associated this ‘sex role debate’ with the independent civic organisation Association 9, this article focuses on the wider gender role movement. The article analyses the interplay and differences among the Finnish Women’s Democratic League, the Committee for Women’s Status, and Association 9’s grassroots activism between 1965 and 1970. It demonstrates that similar ideas about sex roles were presented simultaneously in two public spheres: the dominant public, where the ideas were promoted by Association 9, and the people’s democratic counterpublic, which …
"Emansipaation kriittinen teoreetikko" : Herbert Marcusen poliittinen ajattelu kriittisen teorian edustajana ja uuden vasemmiston opiskelijaradikaali…
2007
Tutkimuksessani tarkastelen Frankfurtin koulukunnan kriittisen teorian edustaja Herbert Marcusea 1960-luvun lopun länsimaisen uuden vasemmiston opiskelijaradikaalien kiistel-tynä isähahmona. Hahmottelen saksalaisen kriittisen filosofian vallankumouksellisuuteen elämäkseen ja 1960-luvun lopulla opiskelijoiden liikehdintään sitoutuneen Marcusen filoso-fisesta ajattelusta ja poliittisesta toiminnasta rakentunutta poliittista figuuria kolmella erityi-sellä lukutavalla. Oletan, että Marcusen ajattelussa on taipumuksia jatkuvuuteen ja sen eri vaiheita ohjaa pyrkimys kapitalistisen järjestelmän kritiikkiin sekä kumoamiseen saksalai-sen negatiivisen filosofian tradition avulla. Näin Marcusen 1960-l…