Search results for "LAB"
showing 10 items of 7932 documents
In Candida parapsilosis the ATC1 Gene Encodes for an Acid Trehalase Involved in Trehalose Hydrolysis, Stress Resistance and Virulence
2014
An ORF named CPAR2-208980 on contig 005809 was identified by screening a Candida parapsilosis genome data base. Its 67% identity with the acid trehalase sequence from C. albicans (ATC1) led us to designate it CpATC1. Homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two CpATC1 chromosomal alleles. Phenotypic characterization showed that atc1Δ null cells were unable to grow on exogenous trehalose as carbon source, and also displayed higher resistance to environmental challenges, such as saline exposure (1.2 M NaCl), heat shock (42°C) and both mild and severe oxidative stress (5 and 50 mM H2O2). Significant amounts of intracellular trehalose were …
Dynamics of fungal colonization in a new medical mycology laboratory
2012
International audience; Objective of the study. - Study of the spatio-temporal fungal colonization in a new medical mycology laboratory. Methods. - A 17-month survey of airborne fungal contamination was conducted in a new medical mycology laboratory at a tertiary care university hospital. This survey was implemented at three different periods: before the new premises were occupied (period A), during the move into the new laboratory (period B) and after resumption of the mycological activities in these new premises (period C). Results. - During period A, the airborne fungal load ranged from 2.3 to 6 cfu/m(3). The most frequently recovered airborne fungi were Penicillium spp. (75 to 100%). Du…
Study of the potential toxicity of commercial crispy breads by evaluation of bioaccessibility and bioavailability of minor Fusarium mycotoxins
2011
Abstract Enniatins (ENs) are bioactive compounds produced by the secondary metabolism of several Fusarium strains and known to have several biological activities, such as acting as enzyme inhibitors, antifungal and antibacterial agents, and immunomodulatory substances. This study has investigated the ENs bioaccessibility, spiked in commercial wheat crispy bread at 1.5 and 3.0 μmol/g concentrations, their transepithelial transport and bioavailability using Caco-2 cells as a model of the human intestinal epithelium. The content (%) of the four ENs contained in the gastric fluid has resulted variable from 69% to 91%, considering the two concentrations assayed. The mean bioaccessibility data fo…
Study of the potential toxicity of enniatins A, A(1), B, B(1) by evaluation of duodenal and colonic bioavailability applying an in vitro method by Ca…
2010
Abstract The bioavailability of the minor Fusarium mycotoxins enniatins (ENs) utilizing an in vitro method which allows the simulation of the small and large intestine tracts has been studied. This method, based on the application of the Caco-2 cells grown alone or in symbiosis with several strains characteristics of the gastrointestinal tract, has permitted to simulate the duodenal and colonic intestinal compartments, respectively. The duodenal bioavailability expressed as absorption value after 4 h of exposure, ranged from 57.7 to 76.8% for EN A, from 68.8 to 70.2% for EN A1, from 65.0 to 67.0% for EN B, and from 62.2 to 65.1% for EN B1. Colonic bioavailability after 48 h of incubation ra…
Bioaccessibility and bioavailability of fumonisin B2 and its reaction products with isothiocyanates through a simulated gastrointestinal digestion sy…
2014
Abstract Fumonisins (FBs) are toxins produced mainly by the molds Fusarium verticillioides (also known as Fusarium moniliforme) and Fusarium proliferatum. These mycotoxins are contaminants of wheat, maize, maize-based foods and other grains worldwide. Isothiocyanates (ITCs) are natural compounds produced by the enzymatic hydrolysis of glucosinolates, which are found in plants of the Brassicaceae family. The use of ITCs as food preservatives has been extensively researched. In this study, allyl (AITC), phenyl (PITC) and benzyl isothiocyanates (BITC) fumigation systems (500 μL/L) were employed to reduce FB2 levels naturally produced in bread by Gibberella moniliformis CECT 2987. Reaction prod…
Unsupervised tissue classification of brain MR images for voxel-based morphometry analysis
2016
In this article, a fully unsupervised method for brain tissue segmentation of T1-weighted MRI 3D volumes is proposed. The method uses the Fuzzy C-Means (FCM) clustering algorithm and a Fully Connected Cascade Neural Network (FCCNN) classifier. Traditional manual segmentation methods require neuro-radiological expertise and significant time while semiautomatic methods depend on parameter's setup and trial-and-error methodologies that may lead to high intraoperator/interoperator variability. The proposed method selects the most useful MRI data according to FCM fuzziness values and trains the FCCNN to learn to classify brain’ tissues into White Matter, Gray Matter, and Cerebro-Spinal Fluid in …
Laboratoriotöiden tavoitteiden saavuttaminen fysiikan perus- ja aineopintokursseilla : kvantitatiivinen tutkimus opiskelijoiden kokemuksista
2017
Tämän työn tarkoituksena oli selvittää opiskelijoiden kokemuksia fysiikan perus- ja aineopintokurssien laboratoriotöiden hyödyllisyydestä. Tutkimus toteutettiin kyselyaineistolla, joka kerättiin Jyväskylän yliopiston fysiikan laitoksen oppilaslaboratoriossa kevään ja kesän 2016 aikana. Opiskelijat vastasivat kyselyyn vapaaehtoisesti suoritettuaan jonkin laboratoriotyön. Kerätyn aineiston pohjalta (\(N=154\) ) analysoitiin kvantitatiivisesti opiskelijoiden kokemuksia kehittymisestä viidessä keskeisessä laboratoriotyön tavoitteessa. Tämän lisäksi aineistosta etsittiin koettuun hyötyyn yhteydessä olevia taustamuuttujia moniluokitteluanalyysillä (MCA). Saatujen tulosten perusteella opiskelijat …
GABAA agonista muscimola ietekme uz žurku atmiņu alcheimera slimības tipa modelī
2015
Darba mērķis bija noskaidrot GABAA agonista muscimola mazo devu ietekmi uz žurku atmiņu icv STZ AD tipa modelī. Lai noteiktu žurku iemācīšanos un atmiņu, tika veikti ūdens labirinta treniņtesti un fināla tests. Rezultāti rāda, ka icv STZ ievade žurkām izraisīja telpiskās atmiņas traucējumus. Muscimols abās devās (0,01 un 0,05 mg/kg) aizsargāja žurkas no STZ izraisītiem efektiem ūdens labirinta testā, uzlabojot to telpisko atmiņu, iemācīšanos un lokomotoro aktivitāti. Iegūtie dati ļauj secināt, ka muscimola mazo devu potenciāls ir intriģējošs jauno anti-AD terapiju izpētē un, ka būtu nepieciešama muscimola hronisko efektu izpēte minētajā modelī.
Secure and scalable dataprocessing and communications for an Animal Tracking System
2003
Masteroppgave i informasjons- og kommunikasjonsteknologi 2003 - Høgskolen i Agder, Grimstad Modern technology is constantly being employed in new fields, by new industries and people. The cost of advanced technological equipment decreases continously, and equipment that was reserved for people and corporations with a lot of money has now become household products. This enables many interesting ideas and projects, Kitron Development’s radiocollar project being one of them. Kitron Development is involved in a project to track the position of animals by substituting the traditional bell collar with a collar that contains a GPS receiver. The collar transmits its position to a base station using…
The Monte San Nicola section (Sicily) revisited: A potential unit-stratotype of the Gelasian Stage
2022
The Monte San Nicola area (Southern Sicily) offers a spectacular exposure of open-marine sediments that were employed in 1998 for defining the Global Stratotype Section and Point (GSSP) of the Gelasian Stage (Upper Pliocene). After the lowering of the Pliocene/Pleistocene boundary to ca. 2.6 Ma in 2010, the Gelasian GSSP has been redefined as the base of both the Pleistocene Series and the Quaternary Period, which increased its importance and visibility within the scientific community. However, documentation on the Monte San Nicola reference section is still sparse. In the light of its renewed status, we decided to undertake a complete revision of the Gelasian Stage in its type area, in ord…