Search results for "LAB"

showing 10 items of 7932 documents

Effect of processing and food matrix on calcium and phosphorous bioavailability from milk-based fruit beverages in Caco-2 cells

2011

9 páginas, 4 figuras, 4 tablas.-- et al.

food.ingredientChemistryPhosphorusIn vitro digestionchemistry.chemical_elementfood and beveragesPhosphorusMilk-based fruit beveragesCalciumBioavailabilityPascalizationfluids and secretionsfoodCaco-2Skimmed milkHigh-pressure processingCalciumFood scienceSolubilityDigestionCaco-2 cellsFood Science
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F-type lectin from the sea bass (Dicentrarchus labrax): purification, cDNA cloning, tissue expression and localization, and opsonic activity.

2009

Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. The F-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. Here we describe the purification, biochemical and molecular properties, and the opsonic activity of an F-type lectin (DlFBL) isolated from sea bass (Dicentrarchus labrax) serum. DlFBL exhibits two tandemly arranged carbohydrate-r…

food.ingredientDNA ComplementaryImmunoblottingAquatic ScienceChromatography AffinityBass (fish)F-type lectin; Dicentrarchus labrax;teleost;emaggluthinins opsoninfoodPhagocytosisOpsonin ProteinsComplementary DNALectinsEnvironmental ChemistryAnimalsDicentrarchus labraxRNA MessengerSea bassCloning MolecularOpsoninemaggluthinins opsoninPhylogenyteleostbiologyBase SequenceLectinGeneral MedicineOpsonin Proteinsbiology.organism_classificationMolecular biologyGene Expression RegulationImmunologybiology.proteinMacrophages PeritonealF lectin sea bass inflammationDicentrarchusBassElectrophoresis Polyacrylamide GelSequence motifF-type lectinFishshellfish immunology
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Iron Bioavailability in Fortified Fruit Beverages Using Ferritin Synthesis by Caco-2 Cells

2008

The bioavailability of iron from fortified fruit beverages was estimated by an in vitro system including enzymatic digestion, iron uptake by Caco-2 cells, and ferritin formation determined via an enzyme immunoassay (ELISA). Thus, the aim of the present study was to assess iron bioavailability as influenced by the presence of known dietary promoter and inhibitory factors in fortified fruit beverages containing iron and/or zinc and/or skimmed milk. No negative effect (p > 0.05) derived from micronutrient interaction can be ascribed to zinc supplementation on iron availability. Besides, the presence of caseinophosphopeptides derived from casein hydrolysis during digestion may confer enhancing …

food.ingredientIronBiological Availabilitychemistry.chemical_elementEnzyme-Linked Immunosorbent AssayZincBeveragesHydrolysisfoodCaseinSkimmed milkAnimalsHumansFood sciencebiologyfood and beveragesGeneral ChemistryMicronutrientBioavailabilityFerritinZincMilkchemistryFruitFerritinsFood Fortifiedbiology.proteinCaco-2 CellsGeneral Agricultural and Biological SciencesDigestionJournal of Agricultural and Food Chemistry
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Bioaccesibility of Cylindrospermopsin from cooked fish muscle after the application of an in vitro digestion model and its bioavailability.

2017

Humans can be exposed to cyanotoxins through the ingestion of contaminated water, food or beverages. In the present work, the bioaccesibility of Cylindrospermopsin (CYN), one of the most relevant cyanotoxins, was evaluated in a pure CYN solution and cooked CYN-contaminated fish muscles (20 μg/mL). An in vitro digestion model including the salivar, gastric, duodenal and colonic phases was performed, being each fraction analyzed by HPLC-MS-MS to evaluate CYN degradation. Moreover, Caco-2/TC7 cells were exposed to the digested duodenal and colonic phases to elucidate the final bioavailability of CYN in an approximation to the real human exposure scenario. The results revealed that CYN bioacces…

food.ingredientMeatBacterial ToxinsSteamingBiological AvailabilityFood Contamination010501 environmental sciencesBiologyToxicology01 natural sciencesModels Biologicalchemistry.chemical_compound0404 agricultural biotechnologyfoodAlkaloidsIngestionAnimalsHumansFood scienceUracil0105 earth and related environmental sciencesCyanobacteria ToxinsMusclesTilapia04 agricultural and veterinary sciencesGeneral MedicineCyanotoxin040401 food scienceBioavailabilityLactic acidGastrointestinal TractOxidative StresschemistryConsumer Product SafetyEnvironmental chemistryDigestionCylindrospermopsinCaco-2 CellsDigestionFood ScienceTilapiaFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
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Effect of cytochrome P450 inhibitors (diethyl dithiocarbamate, ketoconazole and grapefruit juice) on the pharmacokinetics of all-trans-retinoic acid.

2004

Diethyl dithiocarbamate (DEDTC) has been reported to be a more powerful inhibitor of all-trans-retinoic acid (ATRA) in vitro metabolism than the well-established cytochrome P450 (CYP) inhibitor ketoconazole (KC). In recent years grapefruit juice (GJ) has been shown to be able to increase the oral bioavailability of several drugs by inhibiting intestinal CYP. This study investigated the in vivo effect of these CYP inhibitors on the pharmacokinetics of ATRA. The latter was administered to rats as a constant-rate intravenous (i.v.) infusion (0.48 mg h(-1) kg(-1)) during 10 h and orally (1.6 mg kg(-1)). DEDTC (320 mg kg(-1) x 2 i.v., 6.4 and 32 mg kg(-1) per os (p.o.)) did not change the ATRA c…

food.ingredientRetinoic acidPharmaceutical ScienceTretinoinPharmacologyGrapefruit juiceBeverageschemistry.chemical_compoundfoodPharmacokineticsCytochrome P-450 Enzyme SystemIn vivoDrug DiscoverymedicineAnimalsCytochrome P-450 Enzyme InhibitorsEnzyme InhibitorsneoplasmsCytochrome P-450 Enzyme Inhibitorsbiologyorganic chemicalsCytochrome P450BioavailabilityRatsKetoconazolechemistrybiology.proteinKetoconazoleDitiocarbmedicine.drugCitrus paradisiFarmaco (Societa chimica italiana : 1989)
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Bioavailability of calcium from milk-based formulas and fruit juices containing milk and cereals estimated by in vitro methods (solubility, dialyzabi…

2005

An adequate calcium intake during the first years of life is needed for normal growth and development and to prevent rickets. The bioavailability of calcium from infant foods (milk-based formulas and fruit juices containing milk and cereals, FMC), the dietary sources of calcium in these stages of life, has been estimated on the basis of simulated gastrointestinal digestion and calcium solubility and dialyzability values and on the efficiency of transport and uptake by Caco-2 cells. The ranking of samples according to calcium bioavailability depends on the use of solubility or dialyzability as criterion. On the basis of the former, the highest value corresponded to adapted formulas and the l…

food.ingredientchemistry.chemical_elementBiological AvailabilityRicketsCalciumModels BiologicalGastrointestinal digestionBeveragesBass (fish)foodmedicineAnimalsHumansFood scienceSolubilityfood and beveragesBiological TransportGeneral Chemistrymedicine.diseaseIn vitroInfant FormulaBioavailabilityMilkchemistrySolubilityCaco-2FruitCalciumCaco-2 CellsGeneral Agricultural and Biological SciencesEdible GrainDialysisJournal of agricultural and food chemistry
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Task-integrated pair-reading : a study of functional literacy in a foreign language

1997

foreign-language readingfunctional literacypair-readingtask-integrated readinginteractioncollaboration
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El perfil laboral y educativo de los jóvenes psicólogos

2013

En este trabajo se analiza la inserción ocupacional de una muestra de jóvenes profesionales egresados de la Licenciatura en Psicología. Se trata de una temática de gran relevancia y poco abordada hasta la actualidad Su estudio permite conocer la situación de los jóvenes profesionales hoy y su grado de inserción laboral. Este estudio contribuye a la comprensión de las características propias de un campo profesional singular, con rasgos distintivos. A su vez los datos arrojados tienen un alto grado de trasferencia ya que aportan información para evaluar y repensar la formación actual del Psicólogo en la Argentina, considerando sus fortalezas y debilidades, analizando los logros y las deudas p…

formación de postgradolcsh:Psychologyjóvenes psicólogoslcsh:BF1-990inserción laboralSumma Psicológica
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Enseñanza de las ciencias : revista de investigación y experiencias didácticas

2021

Resumen basado en el de la revista El concepto de campo presenta una especial relevancia en Física. Sin embargo, su enseñanza ofrece una serie de dificultades y deficiencias que fueron analizadas por los autores en una investigación previa.De acuerdo con este análisis y la investigación de otros autores se han elaborado algunas propuestas para la introducción de este concepto en educación secundaria. En este estudio se presentan las directrices de la anterior propuesta puesta en práctica en una clase y evaluada después. Los resultados fueron analizados mediante diferentes instrumentos y comparados con los obtenidos por estudiantes que seguían programas oficiales. Cataluña ESP

formación de profesoresenseñanza secundariaGeographySecondary educationaprendizaje por descubrimientoFísicaProfessors FormacióHumanitieselaboración de medios de enseñanzaEducació InvestigacióEducationCiència Ensenyament
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Diseño e implementación de talleres para la cualificación y el empleo. Una experiencia en formación profesional

2018

En esta comunicación se presenta el diseño y desarrollo de diferentes seminarios y talleres para la búsqueda de empleo implementando estrategias de trabajo en equipo. Asumimos que las instituciones educativas tienen que acercar al alumnado en formación al mundo empresarial dotándoles de herramientas para la búsqueda de empleo 2.0 Pretendemos los siguientes objetivos: fomentar el desarrollo de habilidades y potencialidades de los alumnos para que puedan gestionar su propia carrera profesional; Mejorar el perfil profesional del alumnado y sus oportunidades de inserción laboral; Promover la reflexión del alumnado sobre sus propias capacidades y expectativas; y, conocer las diferentes opciones …

formación profesional:PEDAGOGÍA::Organización y planificación de la educación::Formación profesional [UNESCO]UNESCO::PEDAGOGÍA::Organización y planificación de la educación::Formación profesionalempleabilidadinserción sociolaboralinclusión
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