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showing 10 items of 3876 documents

A tissue microarray study of osteosarcoma: histopathologic and immunohistochemical validation of xenotransplanted tumors as preclinical models.

2010

Osteosarcomas (OS) are aggressive neoplasms with a wide range of morphologic patterns.OS cases (primary and xenotransplanted) with paraffin blocks available were collected and included in tissue microarrays (TMAs). A morphologic evaluation including the different passages in mice was carried out according to the new WHO criteria. In addition, TMAs were analyzed with a wide panel of immunohistochemical (IHC) markers (osteonectin, osteocalcin,cytokeratin, S100, Sox-9, Ki-67, Bcl-2, p53, p16, survivin, CD99, and caveolin-1).A total of 61 cases were collected. The distribution of the cases according to the histopathologic pattern was: 38 osteogenic OS, 8 primary chondrogenic OS, 2 primary telan…

MalePathologymedicine.medical_specialtyHistologyCD99OsteocalcinTransplantation HeterologousMice NudePathology and Forensic MedicineCytokeratinMiceSurvivinmedicineBiomarkers TumorAnimalsHumansOsteonectinOsteosarcomaTissue microarraybiologybusiness.industrymedicine.diseaseMicroarray AnalysisImmunohistochemistryTransplantationMedical Laboratory TechnologyDisease Models Animalbiology.proteinCancer researchDisease ProgressionImmunohistochemistryOsteosarcomaFeasibility StudiesOsteonectinbusinessApplied immunohistochemistrymolecular morphology : AIMM
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Supravital Methylene Blue Staining of Piloneural Complexes of Common Fur Hair Follicles in the Rat

2000

Light microscopic observations employing supravital methylene blue staining are presented for piloneural complexes of common fur hairs in the mystacial pad of the rat snout. The investigation revealed anatomical details of piloneural complexes belonging to follicles of both vellus and guard hairs. In the methylene blue stained preparations, different types of palisade-like lanceolate nerve fiber endings could be discriminated. The thicker vellus and thinner guard hairs (hair diameter: 15-25 microm) exhibited a different innervation pattern compared to the thicker guard hairs, and two subtypes of piloneural complexes could be distinguished. Both subtypes were characterized by slightly staine…

MalePathologymedicine.medical_specialtyHistologyNerve fiberBiologyRats Sprague-Dawleychemistry.chemical_compoundmedicineAnimalsColoring AgentsPlexusStaining and Labelingintegumentary systemGeneral MedicineAnatomyRatsStainingMethylene BlueMedical Laboratory Technologymedicine.anatomical_structurechemistryVellus hairFemalesense organsSnoutHair FollicleMethylene blueBiotechnic & Histochemistry
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Multicentre Italian study of SARS-CoV-2 infection in children and adolescents, preliminary data as at 10 April 2020

2020

Data on features of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in children and adolescents are scarce. We report preliminary results of an Italian multicentre study comprising 168 laboratory-confirmed paediatric cases (median: 2.3 years, range: 1 day–17.7 years, 55.9% males), of which 67.9% were hospitalised and 19.6% had comorbidities. Fever was the most common symptom, gastrointestinal manifestations were frequent; two children required intensive care, five had seizures, 49 received experimental treatments and all recovered.

MalePediatricsEpidemiologyProtease InhibitorComorbiditymedicine.disease_causeClinical Laboratory TechniqueSevere Acute Respiratory SyndromeDisease OutbreaksFeces0302 clinical medicineSettore MED/38 - Pediatria Generale E SpecialisticaCOVID-19 TestingRetrospective StudiePandemic030212 general & internal medicineViralChildCoronavirusPediatricDisease OutbreakCoinfectionHospitals PediatricSettore MED/38HospitalsDiarrheaTreatment OutcomeSARS-CoV-2 infection; children; covid-19; hydroxychloroquine; pneumonia; Adolescent; Antiviral Agents; Betacoronavirus; COVID-19; COVID-19 Testing; Child; Child Preschool; Chronic Disease; Clinical Laboratory Techniques; Coinfection; Comorbidity; Coronavirus; Coronavirus Infections; Diarrhea; Disease Outbreaks; Feces; Female; Fever; Hospitals Pediatric; Humans; Immunocompromised Host; Infant; Infant Newborn; Italy; Male; Noninvasive Ventilation; Pandemics; Pneumonia Viral; Protease Inhibitors; Retrospective Studies; SARS-CoV-2; Severe Acute Respiratory Syndrome; Treatment OutcomeItalyChild PreschoolCoinfectionFemalemedicine.symptomCoronavirus InfectionsRapid CommunicationHumanDiarrheamedicine.medical_specialtyCOVID-19; ItalyhydroxychloroquineAdolescentFeverCoronaviruPneumonia ViralAntiviral Agents03 medical and health sciencesBetacoronavirusImmunocompromised Hostchildren030225 pediatricsVirologyIntensive caremedicineHumanspneumoniaProtease InhibitorsPreschoolPandemicsRetrospective StudiesAntiviral Agentchildren; covid-19; hydroxychloroquine; pneumonia; SARS-CoV-2 infection; Adolescent; Antiviral Agents; Betacoronavirus; Child; Child Preschool; Chronic Disease; Clinical Laboratory Techniques; Coinfection; Comorbidity; Coronavirus; Coronavirus Infections; Diarrhea; Disease Outbreaks; Feces; Female; Fever; Hospitals Pediatric; Humans; Immunocompromised Host; Infant; Infant Newborn; Italy; Male; Noninvasive Ventilation; Pandemics; Pneumonia Viral; Protease Inhibitors; Retrospective Studies; Severe Acute Respiratory Syndrome; Treatment OutcomeNoninvasive VentilationBetacoronaviruPandemicbusiness.industryClinical Laboratory TechniquesCoronavirus InfectionSARS-CoV-2pneumonia.SARS-CoV-2 infectionPublic Health Environmental and Occupational HealthInfant NewbornCOVID-19InfantRetrospective cohort studymedicine.diseaseNewbornComorbidityCoronavirusPneumoniaChronic DiseaseFecebusiness
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Validation of STA-Liatest D-Di assay for exclusion of pulmonary embolism according to the latest Clinical and Laboratory Standard Institute/Food and …

2017

: Combined clinical pretest probability (PTP) and D-dimer testing have great diagnostic value for pulmonary embolism exclusion. To harmonize performance levels of D-dimer assays available on the market, the Clinical and Laboratory Standard Institute (CLSI) has published a guideline, endorsed by the US Food and Drug Administration (FDA). Such guideline specifies the ideal D-dimer assay characteristic and target population. This study was conducted following the CLSI guideline to upgrade the assay-intended use and obtain FDA clearance of STA-Liatest D-Di assay for pulmonary embolism exclusion in patient with low/moderate PTP. This was an international, multicenter, prospective nonrandomized, …

MalePediatricsmedicine.medical_specialtypulmonary embolism030204 cardiovascular system & hematologyFood and drug administrationFibrin Fibrinogen Degradation Products03 medical and health sciences0302 clinical medicinemedicineHumans030212 general & internal medicineProspective StudiesSTA-Liatest D-Dibusiness.industryUnited States Food and Drug AdministrationClinical and Laboratory Standard InstituteFood and Drug AdministrationHematologyGeneral MedicineGuidelineOriginal ArticlesMiddle Agedmedicine.diseaseThrombosisConfidence intervalUnited StatesPulmonary embolismPre- and post-test probabilityD-dimerEmergency medicineAmbulatoryBiological AssayFemalebusinessVenous thromboembolismBlood coagulationfibrinolysis : an international journal in haemostasis and thrombosis
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Global, regional, and country-specific lifetime risks of stroke, 1990 and 2016

2018

Background: The lifetime risk of stroke has been calculated in a limited number of selected populations. We sought to estimate the lifetime risk of stroke at the regional, country, and global level using data from a comprehensive study of the prevalence of major diseases.Methods: We used the Global Burden of Disease (GBD) Study 2016 estimates of stroke incidence and the competing risks of death from any cause other than stroke to calculate the cumulative lifetime risks of first stroke, ischemic stroke, or hemorrhagic stroke among adults 25 years of age or older. Estimates of the lifetime risks in the years 1990 and 2016 were compared. Countries were categorized into quintiles of the sociode…

MalePercentileNutrition and DiseaseDisease030204 cardiovascular system & hematologyGlobal HealthSocioeconomic FactorGlobal Burden of Disease0302 clinical medicinepreventionVoeding en ZiekteCause of DeathGlobal healthStrokePOPULATIONCause of deathAged 80 and overeducation.field_of_studyIncidence (epidemiology)IncidenceMedicine (all)11 Medical And Health SciencesGeneral MedicineMiddle Agedlifetime riskstroke3142 Public health care science environmental and occupational health3. Good healthStrokeGBD 2016 Lifetime Risk of Stroke CollaboratorsFemaleBURDENLife Sciences & BiomedicineResearch ArticleHumanAdultRiskPopulationGlobal Burden of Disease (GBD)03 medical and health sciencesMedicine General & InternalAge DistributionGeneral & Internal MedicinemedicineHumansLife SciencePoint estimationcardiovascular diseasesSex DistributioneducationVLAGAgedScience & TechnologyHYPERTENSIONbusiness.industrymedicine.diseaseSocioeconomic Factorsbusiness030217 neurology & neurosurgeryRCDemography
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Exposure to elevated temperatures and risk of preterm birth in Valencia, Spain

2014

Abstract Background Prematurity is the second-leading cause of death in children under the age of 5 worldwide. It is predicted that the future climate will have more intense, longer lasting and frequent extreme heat episodes, and so the temperature effect on the risk of preterm birth is generating considerable interest in the public health field. Our aim was to explore the potential short-term effects of elevated temperatures on the risk of preterm birth in Valencia (Spain). Methods All singleton natural births born in the metropolitan area of Valencia during the warm season (May–September, 2006–2010) were included ( N =20,148). We applied time-series quasi-Poisson generalized additive mode…

MalePercentilePregnancyHot TemperatureSingletonbusiness.industryClimateGestational agemedicine.diseaseBiochemistryExtreme heatApparent temperatureObstetric Labor PrematureIncreased riskHistory 16th CenturyPregnancyRisk FactorsSpainmedicineHumansFemalebusinessGeneral Environmental ScienceCause of deathDemographyEnvironmental Research
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Global, regional, and national progress towards Sustainable Development Goal 3.2 for neonatal and child health: all-cause and cause-specific mortalit…

2021

Background: Sustainable Development Goal 3.2 has targeted elimination of preventable child mortality, reduction of neonatal death to less than 12 per 1000 livebirths, and reduction of death of children younger than 5 years to less than 25 per 1000 livebirths, for each country by 2030. To understand current rates, recent trends, and potential trajectories of child mortality for the next decade, we present the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 findings for all-cause mortality and cause-specific mortality in children younger than 5 years of age, with multiple scenarios for child mortality in 2030 that include the consideration of potential effects of COVID-…

MalePerinatal careNewborn careSTILLBIRTHSRJ101UNDER-5 MORTALITYPsychological intervention010501 environmental sciencesInfant mortalityGlobal Health01 natural sciences0302 clinical medicineRA0421Cause of DeathInfant MortalityGlobal healthLife TablesHealthcare improvements030212 general & internal medicine610 Medicine & healthChildCause of Death; Child; COVID-19; Global Health; Humans; Infant; Life Tables; SARS-CoV-2; Sustainable Development11 Medical and Health SciencesCause of deathLife TableMortality rate1. No povertyPublic Health Global Health Social Medicine and EpidemiologyGeneral MedicineArticlesHälsovetenskaperSustainable DevelopmentMortality analyses3. Good healthPeer reviewChild PreschoolSDG 1 - No PovertyChild MortalityFemaleLife Sciences & BiomedicineHumanChild mortalityCOUNTRIESDEATHSInfants -- MortalitatGBD610 Medicine & health03 medical and health sciencesMedicine General & InternalSDG 3 - Good Health and Well-beingLife tablesunder-5 mortality rateGeneral & Internal MedicineHealth SciencesNeonatal deathsmedicineSYSTEMATIC ANALYSISHumans:Medicine [Science]Infants -- SalutPreschool0105 earth and related environmental sciencesScience & TechnologyInfants nadons -- Salutbusiness.industrySARS-CoV-2Infants nadons -- MortalitatINFORMInfant NewbornCOVID-19InfantNeonatal and child health ; Sustainable Development Goal 3.2 ; Global healthGBD 2019 Under-5 Mortality Collaboratorsmedicine.diseaseNewbornTRENDSInfant mortalitySustainable Development GoalChild mortalityFolkhälsovetenskap global hälsa socialmedicin och epidemiologiDeaths3121 General medicine internal medicine and other clinical medicineRGbusinessSystematic AnalysisMalariaDemography
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Method for functional study of mitochondria in rat hypothalamus

2008

1872-678X (Electronic) Journal Article Research Support, Non-U.S. Gov't; Different roles of mitochondria in brain function according to brain area are now clearly emerging. Unfortunately, no technique is yet described to investigate mitochondria function in specific brain area. In this article, we provide a complete description of a procedure to analyze the mitochondrial function in rat brain biopsies. Our two-step method consists in a saponin permeabilization of fresh brain tissues in combination with high-resolution respirometry to acquire the integrated respiratory rate of the biopsy. In the first part, we carefully checked the mitochondria integrity after permeabilization, defined exper…

MalePermeability/drug effectsWistarMitochondrionRespirometry0302 clinical medicineHyperglycemia/physiopathologyMitochondria/*physiology/ultrastructurePhosphorylationComputingMilieux_MISCELLANEOUS0303 health sciencesMicroscopymedicine.diagnostic_testGeneral NeuroscienceBrainFastingBrain/drug effects/physiology3. Good healthCell biologyMitochondriaLaboratory Techniques and ProceduresZuckerCell typeCellular respirationPhysiologicalCell RespirationHypothalamusOxidative phosphorylation[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyStressElectronPermeability03 medical and health sciencesFasting/physiologyOxygen ConsumptionStress PhysiologicalBiopsyRespirationmedicineAnimalsRats Wistar[SDV.BC] Life Sciences [q-bio]/Cellular Biology030304 developmental biologyClinical Laboratory TechniquesSaponinsRats ZuckerRatsMicroscopy ElectronHypothalamus/drug effects/*physiology/ultrastructureHyperglycemiaSaponins/pharmacologyNeuroscience030217 neurology & neurosurgeryFunction (biology)
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Toxicological Profile of Ultrapure 2,2´,3,4,4´,5,5´-Heptachlorbiphenyl (PCB 180) in Adult Rats

2014

PCB 180 is a persistent non-dioxin-like polychlorinated biphenyl (NDL-PCB) abundantly present in food and the environment. Risk characterization of NDL-PCBs is confounded by the presence of highly potent dioxin-like impurities. We used ultrapure PCB 180 to characterize its toxicity profile in a 28-day repeat dose toxicity study in young adult rats extended to cover endocrine and behavioral effects. Using a loading dose/maintenance dose regimen, groups of 5 males and 5 females were given total doses of 0, 3, 10, 30, 100, 300, 1000 or 1700 mg PCB 180/kg body weight by gavage. Dose-responses were analyzed using benchmark dose modeling based on dose and adipose tissue PCB concentrations. Body w…

MalePhysiologyAdipose tissueTHYROID-HORMONEPOSTNATAL EXPOSURE010501 environmental sciences413 Veterinary scienceToxicologyPathology and Laboratory Medicine01 natural sciencesBiochemistryRats Sprague-DawleyFollicle-stimulating hormoneHemoglobinsMedicine and Health SciencesEFFECT-DIRECTED ANALYSIS0303 health scienceseducation.field_of_studyMultidisciplinaryBehavior AnimalMaintenance doseQRNeurochemistryAnemiaNeurotransmittersHematologyPolychlorinated BiphenylsToxicokineticsAdipose TissueHematocritLiverToxicityBlood ChemistryMedicineEnvironmental PollutantsFemaleLuteinizing hormoneResearch ArticleARYL-HYDROCARBON RECEPTORNeurotoxicologymedicine.medical_specialtyThyroid HormonesPOLYCHLORINATED-BIPHENYLS PCBSScienceeducationPopulationToxic Agentsta3111Loading dose03 medical and health sciencesRetinoidsSex FactorsInternal medicinemedicineSex HormonesDEVELOPMENTAL EXPOSUREAnimalseducationToxic equivalency factorMolecular Biology030304 developmental biology0105 earth and related environmental sciencesToxicityDose-Response Relationship DrugDIBENZO-P-DIOXINSBody WeightBiology and Life SciencesIN-VITROKemiLuteinizing HormoneHormonesRatsDIOXIN-LIKE-PCBSEndocrinologyChemical SciencesAdrenal CortexExploratory BehaviorSUBCHRONIC TOXICITYFollicle Stimulating HormoneDNA Damage
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An investigation of plasma interleukin-6 in sport-related concussion

2020

Background Increasing evidence suggests inflammation is an important component of concussion pathophysiology. However, its etiology, restitution, and potential clinical repercussions remain unknown. The purpose of the current study was to compare the blood concentrations of interleukin (IL) -6, a prominent inflammatory cytokine, between healthy athletes and athletes with a sport-related concussion (SRC), while addressing the potential confounds of sex, recent physical activity, and the interacting effect of concussion history. Method A prospective, observational cohort study was conducted on athletes at a single academic institute participating across 13 interuniversity sports. Follow-up of…

MalePhysiologySocial SciencesPathology and Laboratory Medicine0302 clinical medicineImmune PhysiologyConcussionMedicine and Health SciencesPsychologyPublic and Occupational HealthProspective StudiesYoung adultProspective cohort studyImmune ResponseInnate Immune System0303 health sciencesMultidisciplinarybiologyQRSports ScienceBody FluidsBloodAthletic InjuriesRegression AnalysisCytokinesMedicineFemaleAnatomyResearch ArticleSportsCohort studymedicine.medical_specialtyScienceImmunologyBlood PlasmaYoung Adult03 medical and health sciencesSigns and SymptomsDiagnostic MedicineInternal medicinemedicineHumansSports and Exercise MedicineInterleukin 6Brain Concussion030304 developmental biologyInflammationBehaviorInterleukin-6Athletesbusiness.industryCase-control studyBiology and Life SciencesPhysical ActivityMolecular Developmentmedicine.diseasebiology.organism_classificationCase-Control StudiesImmune SystemEtiologybiology.proteinRecreationPhysiological ProcessesSleepbusiness030217 neurology & neurosurgeryDevelopmental BiologyPLOS ONE
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