Search results for "LACTATE"
showing 10 items of 363 documents
Physiological effects of tricyclazole on zebrafish (Danio rerio) and post-exposure recovery
2009
Short-term effects of tricyclazole on male zebrafish (Danio rerio) physiology were examined joint to the degree of recovery after exposure. Fish were exposed to 142 microg/L (1/100 LC(50)-96 h) of tricyclazole for 7 (Exp.1) and 14 days (Exp.2) and then allowed to recover for 7 or 14 more days, respectively. Whole-body triglycerides, cholesterol, glucose, lactate and total proteins were measured as well as the aspartate aminotransferase (AAT), alanine aminotransferase (AlAT), alkaline phosphatase (AP) and lactate dehydrogenase (LDH) activities as biomarkers of intermediary metabolism; gamma-glutamyl transpeptidase (gammaGT) as biomarker of oxidative detoxification processes and vitellogenin …
Effects of 8 weeks' endurance training on skeletal muscle metabolism in 56?70-year-old sedentary men
1977
The effects of 8 weeks' endurance training on muscle metabolism at rest and after a submaximal bicycle ergometer exercise were studied in 31 previously sedentary men, aged 56-70. Training consisted of 3-5 one hour exercise bouts per week including walking-jogging, swimming, gymnastics and ball games. The effects of training were similar to those previously reported for younger men. Mean maximal oxygen uptake increased (11%), as did the resting values for muscle glycogen concentration, the enzymes representing aerobic energy metabolism (malate dehydrogenase, succinate dehydrogenase), and also some of the anaerobic enzymes (creatine phosphokinase, lactate dehydrogenase). Lactate production du…
Connective tissue of “fast” and “slow” skeletal muscle in rats…effects of endurance training
1980
The connective tissue of two skeletal muscles having different contractile properties was investigated in trained and untrained rats. The animals to be trained were put to run on a treadmill 5 days a week for 4 weeks. The "slow" m. soleus (MS) showed higher malate dehydrogenase activity but lower lactate dehydrogenase activity compared to the "fast" m. rectus femoris (MRF). When whole muscles were taken into account, the concentrations of both hydroxyproline and hexosamines were higher for MS compared to MRF. In the middle section of MS there were more hexosamines than in that of MRF, but no similar difference existed in hydroxyproline. The histochemical staining of collagen, however, sugge…
Mitochondrial myopathy with lactic acidosis and deficient activity of muscle succinate cytochrome-c-oxidoreductase
1984
A male infant had severe muscular hypotonia from birth. Recurrent vomiting with dehydration and severe metabolic acidosis complicated the course. Elevated lactate (up to 12.3 mmol/l; n less than 2), pyruvate (0.4 mmol/l; n less than 0.05) and alanine levels were found in serum with an abnormal lactate/pyruvate ratio (greater than 30; n less than 15). In urine the concentrations of lactate, pyruvate, alanine and of several intermediates of the citric acid cycle were increased. In muscle, numerous disseminated "ragged red fibres" were found by light microscopy; muscle fibres were found to contain subsarcolemmal aggregates of mitochondria, lipid droplets and glycogen by electromicroscopical me…
Morphological and biochemical changes in striated muscle after experimental tourniquet ischaemia.
1979
Histological and biochemical changes were studied in the striated muscle following total tourniquet ischaemia between one and four h, the reflow time being 30 min and 24 h. Electronmicroscopy was applied to study the fine structure of the muscle after 24 h reflow. In light microscopy ischaemic changes were not seen even when the tourniquet time was extended to four h. When a four-h ischaemia was followed by a 24-h recovery period, the electron microscopy showed a variety of minor mitochondrial changes such as condensed and slightly dilatated mitochondria. The SDH activities did not vary significantly between the experimental and control samples even after a four-h ischaemia followed by 30 m…
Recovery from a national collegiate athletic association division I football game: muscle damage and hormonal status.
2008
The purpose of this study was to examine markers of skeletal muscle tissue damage and circulating anabolic and catabolic hormones to gain insight into the recovery process from Friday until Monday, when a new practice week begins. Twenty-eight National Collegiate Athletic Association Division I football players gave consent to participate in the investigation in the ninth game of the season. Sixteen players started the game and played the entire game (PL), and 12 others did not play and were on the bench during the game (DNP). Each player had fasted blood samples obtained at the same time of day between 1000 and 1200 hours the day before the game (Friday; T1), 18-20 hours after the game (Su…
Short-term exposure to sublethal tebuconazole induces physiological impairment in male zebrafish (Danio rerio).
2010
Abstract The aim of the present study was to assess the physiological response of male zebrafish Danio rerio to the fungicide tebuconazole and recovery in fungicide-free water. Acute toxicity tests were carried out and the median lethal concentration (LC 50 ) from 24 to 96 h was calculated. The fish were exposed to a sublethal fungicide concentration of 230 μg/L for 7 or 14 days and allowed to recover for 7 or 14 more days, respectively. Whole-body levels of vitellogenins, triglycerides, cholesterol, glucose, lactate and proteins as well as the activities γ-glutamil transpeptidase (γ-GT), alanin aminotransferase (AlAT), alkaline phosphatase (AP) and lactate dehydrogenase (LDH) were assayed;…
Reductive Drug Metabolism in Isolated Perfused Rat Liver under Restricted Oxygen Supply
1978
1. Hepatic azo and nitro reductase activities were studied in the perfused rat liver under normal and restricted oxygen supply. 2. Formation of sulphanilamide or p-aminobenzoic acid from neoprontosil or p-nitrobenzoic acid under aerobic conditions of liver perfusion was negligible, even at a reduced oxygen saturation of a pO2 of 300 mm Hg in the haemoglobinfree perfusion system. At a pO2 of 200 mm Hg reductase activities were almost maximal. 3. Conjugation of sulphanilamide (0-08 mM) was similar under aerobic and anaerobic conditions. Hepatic elimination of p-aminobenzoic acid (0-08 mM) showed an oxygen-dependent increase for 15 min after addition of substrate. 4. p-Nitroanisole demethylati…
Blood lactate production and recovery from anaerobic exercise in trained and untrained boys.
1988
Blood lactate production and recovery from anaerobic exercise were investigated in 19 trained (AG) and 6 untrained (CG) prepubescent boys. The exercises comprised 3 maximal test performances; 2 bicycle ergometer tests of different durations (15 s and 60 s), and running on a treadmill for 23.20±2.61 min to measure maximal oxygen uptake. Blood samples were taken from the fingertip to determine lactate concentrations and from the antecubital vein to determine serum testosterone. Muscle biopsies were obtained from vastus lateralis. Recovery was passive (seated) following the 60 s test but that following the treadmill run was initially active (10 min), and then passive. Peak blood lactate was hi…
Effect of endurance training on the capacity of red and white skeletal muscle of mouse to oxidize carboxyl-14C-labelled palmitate.
1977
Three groups of mice were trained for 1, 4 and 5 months according to different running programs on a motor driven treadmill and the fatty acid oxidation capacity (FAO) and the activities of some enzymes of energy metabolism (cytochrome c oxidase, malate dehydrogenase, triosephosphate dehydrogenase, and lactate dehydrogenase) were determined from m. quadriceps femoris (MQF). Endurance training increased the FAO [5-month training 4 days/week, 30 min/day 22% (p less than 0.05); 1-month training, 7 days/week, 150 min/day 37% (p less than 0.001); 4-month training, 5 days/week, 60 min/day 24% (p less than 0.05)]. The activities of cytochrome c oxidase and malate dehydrogenase increased approx. 30…