Search results for "LAMP"
showing 10 items of 457 documents
Sequence, evolution and tissue expression patterns of an epidermal type I keratin from the shark Scyliorhinus stellaris.
2004
From the shark Scyliorhinus stellaris we cloned and sequenced a cDNA encoding a novel type I keratin, termed SstK10. By MALDI-MS peptide mass fingerprinting of cytoskeletal proteins separated on polyacrylamide gels, we assigned SstK10 to a 46-kDa protein which is the major epidermal type I (“IE”) keratin in this fish and is specifically expressed in stratified epithelia. In a phylogenetic tree based on type I keratin sequences and with lamprey keratins applied as outgroup, SstK10 branches off in a rather basal position. This tree strongly supports the concept that teleost keratins and tetrapod keratins resulted from two independent gene radiation processes. The only exception is human K18 b…
Curating Objects from the European Border Zone: The “Lampedusa Refugee Boat”
2019
The cultural politics of the present encourage museums and artists to seek an ethical vision within Europe navigating the knowledge of ongoing mass death at the border. This is one explanation for the interest in objects symbolising present-day irregular border crossing among museum curators, artists, designers and activists. Wooden fishing boats, inflatable dinghies and life jackets appear regularly in exhibitions and installations. This chapter focuses on the meaning of “the Lampedusa boat” and argues that the narrative context within which the boats are exhibited guides the work of imagination that animates the object. While exhibiting the boats carries the critical potential to relocate…
Il Palazzo Cutò di Bagheria
1995
Disegnata dal frate Giuseppe Mariani, la villa-palazzo di Luigi Naselli, Principe di Aragona, fu edificata nella seconda decade del Settecento. Attraverso una originale indagine archivistica la monografia ricostruisce le fasi della sua edificazione, le trasformazioni, l'importante passaggio di proprietà ai Filangeri di Cutò nel 1803, i rifacimenti intervenuti nelle sale interne tra la metà dell'Ottocento e i primi del Novecento, i rapporti con il fenomeno più ampio dell'architettura delle ville. Lo studio si interrompe con il primo dopoguerra, che registra il passaggio dalla proprietà aristocratica - la villa era stata ereditata da Giuseppe Tomasi di Lampedusa - ad alcuni gruppi familiari d…
Investigation of the hyperfine structure of weak atomic Vanadium lines by means of Fourier transform spectroscopy
2015
In continuation of our work on the investigation of the hyperfine structure (HFS) of atomic vanadium, we analyzed weak spectral lines in a Fourier transform spectrum that have not been investigated up to now. The main objective of this work was the determination of the magnetic dipole HFS constant A of the energy level at 15 103.784 cm−1, which was the only energy level with unknown A value up to the energy of 28 000 cm−1. Additionally, other gaps in the data of magnetic dipole HFS could be filled in. The spectrum of vanadium–argon plasma in a hollow cathode lamp is recorded in the spectral range from 12 500 to 26 000 cm−1 or 800 to 380 nm, respectively. The HFS of 42 weak atomic vanadium l…
Coexpression of heat-evoked and capsaicin-evoked inward currents in acutely dissociated rat dorsal root ganglion neurons.
1997
Noxious heat is able to activate heat-sensitive nociceptors in the skin very rapidly, but little is known about the mechanisms by which heat is transduced. We used the whole-cell patch-clamp technique to study the effects of noxious heat and capsaicin on freshly dissociated rat dorsal root ganglion neurons in vitro. Using temperatures between 41 degrees C and 53 degrees C, 8 of 19 small neurons (phior = 30 microm) exhibited a heat-evoked inward current. All heat-sensitive neurons tested were also capsaicin-sensitive. Moreover, the heat response tended to be enhanced after capsaicin (360 +/- 150 pA versus 125 +/- 45 pA, P0.1, n = 7). Two of five heat-insensitive neurons were excited by capsa…
‘In situ’ preparation of nitrous acid solutions by photoreduction in a flow assembly for analysis of pharmaceuticals
2003
The solutions of nitrite ion, or nitrous acid, are frequently used as reagent in different analytical procedures and in pharmaceutical analysis. The low stability of the aqueous solutions of this reagent implies the tedious work of freshly prepared solutions every day and being kept in the refrigerator. In this paper, a photoreactor formed by a low pressure mercury lamp and nesting in a continuous-flow manifold is studied, tested, and proposed for the in situ reduction of nitrate solution into nitrite. The conversion into nitrite is reproducible and it has been successfully applied to determination of different drugs, mostly from the sulphonamide family, in an FIA assembly.
Ergastoplasmic paracrystalline inclusion bodies in the adipose gonadal envelope and fat body of the glow worm, Lampyris noctiluca (Insecta, Coleopter…
2001
Abstract The gonads of glow worm larvae are enveloped by adipose tissue which represents a specialized fat body. The adipose gonadal envelope, and also to a lesser extent the fat body cells, contain tubular paracrystalline inclusion bodies (PIBs). Cells of other tissues are devoid of such inclusions. The PIBs form in the cisternae of rough ER. In young larvae PIB formation is sparse, but at advanced larval stages PIBs often occur as bundles in stacks of ergastoplasm. Typically, a PIB within a cisterna consists of four to seven parallel tubules. The outer diameter of a tubule is ca 28.8 nm and the width of the tubule lumen ca 12.2 nm. The “wall” of a tubule contains globular protein subunits…
Does cyclic GMP mediate the negative inotropic effect of acetylcholine in the heart?
1977
DURING vagal stimulation the pacemaker activity of the heart is diminished. The reduction in heart rate is due to a release of acetylcholine (ACh) from the parasympathetic nerve terminals that increases the permeability of the myocardial cell membrane for potassium ions (for review see ref. 1). This is accompanied by a shortening of the action potential duration in atrial muscle and a diminished calcium uptake2, which in turn results in a negative inotropic effect. Voltage clamp experiments in mammalian atrial muscle have shown that with higher concentrations of ACh not only is the potassium current augmented but also the slow inward current of calcium is reduced3. It is not clear how the c…
Experimental Study of Vibrations in Face Milling Cutting
2014
The paper consists of a research in designing and experimental analysis using a face milling cutter with round inserts. A general model for the new cutter is proposed taking into consideration multiple aspects regarding monitoring and controling the milling process. A comparison of measured vibrations when cutting with different parameters for two constructions of the milling head is presented. The objective of this vibration comparison is to verify wether the new design behaves in similar way with the classic and proven concept of insert clamping using a central screw.
Nei luoghi dell'accoglienza. Progetti per Lampedusa e Palermo
2014
Il libro raccoglie gli esiti della ricerca e della didattica dei Laboratori di Progettazione architettonica del terzo anno, svolti presso il corso di Laurea Magistrale in Architettura LM4 della Scuola Politecnica dell’Università degli Studi di Palermo, sul tema dell’accoglienza a Lampedusa e a Palermo. L’obiettivo è quello di presentare la composita esperienza di progetto e di ricerca prodotta intorno all’argomento e nello stesso tempo attivare un confronto sul paesaggio e sugli spazi urbani della città. Il libro cerca di sviluppare un percorso di ricognizione e di conoscenza attraverso l’elaborazione di progetti didattici in grado di produrre idee per la città e il territorio. The book con…