Search results for "LAMP"
showing 10 items of 457 documents
Sequential acquisition of cacophony calcium currents, sodium channels and voltage-dependent potassium currents affects spike shape and dendrite growt…
2014
During metamorphosis the CNS undergoes profound changes to accommodate the switch from larval to adult behaviors. In Drosophila and other holometabolous insects, adult neurons differentiate either from respecified larval neurons, newly born neurons, or are born embryonically but remain developmentally arrested until differentiation during pupal life. This study addresses the latter in the identified Drosophila flight motoneuron 5. In situ patch-clamp recordings, intracellular dye fills and immunocytochemistry address the interplay between dendritic shape, excitability and ionic current development. During pupal life, changes in excitability and spike shape correspond to a stereotyped, progr…
Maternal complications in pregnancy and wheezing in early childhood: a pooled analysis of 14 birth cohorts
2015
Background: Evidence on the effect of maternal complications in pregnancy on wheezing in offspring is still insufficient. Methods: A pooled analysis was performed on individual participant data from fourteen European birth cohorts to assess the relationship between several maternal pregnancy complications and wheezing symptoms in the offspring. Exposures of interest included hypertension and preeclampsia, diabetes, as well as pre-pregnancy overweight (body mass index between 25 and 29.9) and obesity (body mass index >= 30) compared with normal weight (body mass index between 18.5 and 24.9). Outcomes included both ever and recurrent wheezing from birth up to 12-24 months of age. Cohort-speci…
Two-Stage Origin of K-Enrichment in Ultrapotassic Magmatism Simulated by Melting of Experimentally Metasomatized Mantle
2019
The generation of strongly potassic melts in the mantle requires the presence of phlogopite in the melting assemblage, while isotopic and trace element analyses of ultrapotassic rocks frequently indicate the involvement of subducted crustal lithologies in the source. However, phlogopite-free experiments that focus on melting of sedimentary rocks and subsequent hybridization with mantle rocks at pressures of 1&ndash
Influence of Uniaxial Pressure on Dielectric Properties and Aging Effect of BiFeO3Ceramic
2015
The external stress (0–1500 bar) dependence of dielectric properties and an aging effect of BiFeO3 ceramic has been investigated. The electric permittivity and dielectric losses first increased (up to about 700 bar) and next decreased with uniaxial pressure applied parallel to the ac field direction, while increased with the stress perpendicularly applied. It was suggested that combination of the de-aging, clamping of the domain walls, and the polarization reorientation mechanisms can be responsible for these changes. The aging effect is followed logarithmic law and is related to the relaxation of the domain structure towards an equilibrium configuration.
The main determinant of furosemide inhibition on GABA(A) receptors is located close to the first transmembrane domain.
1998
Inhibitory GABA(A) receptors are regulated by numerous allosteric modulators, the most receptor-subtype specific of which is furosemide. It recognises receptors of the subunit composition alpha6beta2/3gamma2, restricted to cerebellar granule cells. To locate furosemide's site of action we constructed chimeras of the furosemide-sensitive alpha6 and the furosemide-insensitive alpha1 subunit, and expressed and studied them together with the beta3 and gamma2 subunits in Xenopus oocytes by the two-electrode voltage clamp technique. The inhibition of GABA-induced currents by furosemide mainly depended on a short domain proximal to the first transmembrane region of the alpha6 subunit.
Statins and angiogenesis in non-cardiovascular diseases.
2022
Statins inhibit HMG-CoA reductase by competitively inhibiting the active site of the enzyme, thus preventing cholesterol synthesis and reducing the risk of developing cardiovascular disease. Many pleiotropic effects of statins have been demonstrated that can be either related or unrelated to their cholesterol-lowering ability. Among these effects are their proangiogenic and antiangiogenic properties that could offer new therapeutic applications. In this regard, pro- and anti-angiogenic properties of statins have been shown to be dose dependent. Statins also appear to have a variety of non-cardiovascular angiogenic effects in many diseases, some examples being ocular disease, brain disease, …
Action of tertiary phenylalkylamines on cardiac transient outward current from outside the cell membrane.
1996
The effects of the phenylalkylamines verapamil (V), gallopamil (G), and devapamil (D) and their corresponding quaternary derivatives on the transient outward current (Ito) were examined in rat ventricular cardiomyocytes using the whole-cell patch-clamp technique. The question was addressed, whether phenylalkylamines act on Ito from the inside or the outside or from both sides of the cell membrane. To this end, the myocytes were either superfused extracellularly or perfused intracellularly with drug-containing solutions. In addition, the effects of verapamil were investigated at different pH-values. V, G, and D (30 microM each), applied extracellularly, reduced the steady state current of It…
Barnidipine block of L-type Ca2+ channel currents in rat ventricular cardiomyocytes
2000
The effects of barnidipine and nifedipine on L-type Ca2+ current (ICa(L)) were investigated in ventricular cardiomyocytes from rats. Both barnidipine and nifedipine reduced ICa(L) in a concentration and voltage dependent manner; the EC50 were 80 and 130 nM at a holding potential of −80 mV, respectively, and 18 and 6 nM at −40 mV, respectively. Both drugs induced a leftward shift of the steady-state inactivation curve of ICa(L). Using a twin pulse protocol, the relationships between the amount of block of ICa(L) by either drug, seen during the second pulse, and the length of the first pulse were described by monoexponential functions reflecting onset of block, dependent on drug concentration…
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium
2019
Background Voltage-gated sodium (Nav) channels have traditionally been considered a trademark of excitable cells. However, recent studies have shown the presence of Nav channels in several non-excitable cells, such as astrocytes and macrophages, demonstrating that the roles of these channels are more diverse than was previously thought. Despite the earlier discoveries, the presence of Nav channel-mediated currents in the cells of retinal pigment epithelium (RPE) has been dismissed as a cell culture artifact. We challenge this notion by investigating the presence and possible role of Nav channels in RPE both ex vivo and in vitro. Results Our work demonstrates that several subtypes of Nav cha…
Tunable structured illumination system based on a Wollaston prism
2018
Experimental verification of a simple illumination system to generate a 1D structured pattern with tunable modulation frequency is shown based on a Wollaston prism illuminated by the diffracted field of an incoherent linear source.