Search results for "LAMP"

PACAP induces bradycardia in guinea-pig heart by stimulation of atrial cholinergic neurones

1996

Based on previous studies which indicated that pituitary adenylate cyclase activating peptide (PACAP) acts as a positive inotropic and chronotropic substance in different species via the cAMP signal transduction pathway, the objective of the present work was to investigate cAMP-regulated myocardial key proteins in response to PACAP in isolated ventricular cells of the guinea pig. Surprisingly, the two molecular forms of PACAP, PACAP(1-27) and PACAP(1-38), showed no effect on intracellular cAMP-levels, L-type Ca2+ channel current or phosphorylation of troponin inhibitor (TnI) and phospholamban (PLB). Additionally, inotropy of isolated guinea-pig ventricular strips was not affected by the neu…

Properties and roles of BKCa channels in cultured cerebellar granule neuron: Experimental and simulation studies

2006

We studied the properties and roles of large-conductance calcium-sensitive potassium channels (BK"C"a) in cultured rat cerebellar granule neurons. In intact cells these channels had chord conductances of 130-200pS. The conductance, threshold for channel activation and the mean open time and open probability depend on the age of cells. In computer simulations the elimination of BK"C"a channel decreases the maximum and minimum peak values of an action potential, causes reduced hyperpolarization and increases firing frequency. The observed alterations may be linked to the complexity of granule neuron excitability during maturation and provide new insight into modeling the developmental changes…

Electrical resistivity and induced polarization tomographies to test the efficiency and safety of the new landfill of Bellolampo (Palermo, Italy)

2016

A geophysical study by means of electrical resistivity and induced polarization tomographies was carried out in the landfill site of Bellolampo (Palermo, Italy) to check the integrity of the landfill liner and to detect possible leachate plumes. Preliminarily, synthetic tests were performed, simulating acquisition data along profiles placed above resistivity distribution, suitable to represent landfills protected with an high-density polyethylene (HDPE) liner at the bottom or with possible leachate plumes. Optimized array data sets for parallel measurements have been tested in order to assess the resolution and the effectiveness of tomographic images, comparing inversions with and without t…

Evaluation of B-cell secretion and peripheral insulin resistance during pregnancy and after delivery in gestational diabetes mellitus with obesity

1988

Nine pregnant women with gestational diabetes mellitus (GDM) were studied. Six normal pregnant women and six normal nonpregnant women were evaluated as control groups. All the women underwent oral glucose tolerance test and glucose clamp during the third trimester of pregnancy and two months after delivery. During OGTT, glucose, C-peptide and insulin plasma levels were determined. C-peptide and insulin values in the late phase of OGTT were higher during pregnancy than after delivery in both groups. In gestational diabetic women, the M-value in the second steady-state during glucose clamp was lower than in controls, both during pregnancy and after delivery. Nevertheless, in both groups the M…

Effects on α- and β-cell function of sequentially adding empagliflozin and linagliptin to therapy in people with type 2 diabetes previously receiving…

2017

Aims The aim of the study was to investigate the effect of sequential treatment escalation with empagliflozin and linagliptin on laboratory markers of alpha- and beta cell function in patients with type 2 diabetes mellitus (T2DM) insufficiently controlled on metformin monotherapy. Methods Forty-four patients with T2DM received 25 mg empagliflozin for a duration of one month in an open-label fashion (treatment period (TP 1). Thereafter, patients were randomised to a double-blind add-on therapy with linagliptin 5 mg or placebo (TP 2) for one additional month. Alpha- and beta cell function were assessed with a standardised liquid meal test (LMT) and an intravenous (iv.) glucose challenge. Effi…

"Lampedusa und das 'Schwarze Mittelmeer'. Die Migrationsrouten im Jahrtausend der Globalisierung"

2013

On frontiers in the global millennium. The role played by the minor island of Lampedusa as a bridge and border between Africa and Europe.

Transient BK outward current enhances motoneurone firing rates duringDrosophilalarval locomotion

2015

Key points We combine in situ electrophysiology with genetic manipulation in Drosophila larvae aiming to investigate the role of fast calcium-activated potassium currents for motoneurone firing patterns during locomotion. We first demonstrate that slowpoke channels underlie fast calcium-activated potassium currents in these motoneurones. By conducting recordings in semi-intact animals that produce crawling-like movements, we show that slowpoke channels are required specifically in motoneurones for maximum firing rates during locomotion. Such enhancement of maximum firing rates occurs because slowpoke channels prevent depolarization block by limiting the amplitude of motoneurone depolarizati…

ECOLOGIA DELL' IMPOLLINAZIONE DI PERIPLOCA LAEVIGATA SUBSP ANGUSTIFOLIA ( LABILL.) MARKGRAF ( APOCYNACEAE-PERIPLOCOIDEAE ) E CALLUMA EUROPEA N.E.BR. …

2011

Human T cells in silico: Modelling their electrophysiological behaviour in health and disease

2016

Although various types of ion channels are known to have an impact on human T cell effector functions, their exact mechanisms of influence are still poorly understood. The patch clamp technique is a well-established method for the investigation of ion channels in neurons and T cells. However, small cell sizes and limited selectivity of pharmacological blockers restrict the value of this experimental approach. Building a realistic T cell computer model therefore can help to overcome these kinds of limitations as well as reduce the overall experimental effort. The computer model introduced here was fed off ion channel parameters from literature and new experimental data. It is capable of simu…

Type I keratin cDNAs from the rainbow trout: independent radiation of keratins in fish

2002

Five different type I keratins from a teleost fish, the rainbow trout Oncorhynchus mykiss, have been sequenced by cDNA cloning and identified at the protein level by peptide mass mapping using MALDI-MS. This showed that the entire range of type I keratins detected biochemically in this fish has now been sequenced. Three of the keratins are expressed in the epidermis (subtype Ie), whereas the other two occur in simple epithelia and mesenchymal cells (subtype Is). Among the Is keratins is an ortholog of human K18; the second Is polypeptide is clearly distinct from K18. We raised a new monoclonal antibody (F1F2, subclass IgG1) that specifically recognizes trout Is keratins, with negative react…