Search results for "LB"
showing 10 items of 5653 documents
Mouse models of cytomegalovirus latency: overview.
2002
Abstract Background: The molecular regulation of viral latency and reactivation is a central unsolved issue in the understanding of cytomegalovirus (CMV) biology. Like human CMV (hCMV), murine CMV (mCMV) can establish a latent infection in cells of the myeloid lineage. Since mCMV genome remains present in various organs after its clearance from hematopoietic cells first in bone marrow and much later in blood, there must exist one or more widely distributed cell type(s) representing the cellular site(s) of enduring mCMV latency in host tissues. Endothelial cells and histiocytes are candidates, but the question is not yet settled. Another long debated problem appears to be solved: mCMV establ…
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase.
2001
The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), has recently emerged as a promising target for antiviral intervention. Here, we describe the isolation, functional characterization, and molecular cloning of a monoclonal antibody (mAb) inhibiting the HCV RdRp. This mAb, designated 5B-12B7, binds with high affinity to a conformational epitope in the palm subdomain of the HCV RdRp and recognizes native NS5B expressed in the context of the entire HCV polyprotein or subgenomic replicons. Complete inhibition of RdRp activity in vitro was observed at equimolar concentrations of NS5B and mAb 5B-12B7, whereas RdRp activities of classica…
Replication of herpes simplex virus type 1 and 2 in the medulla of the adrenal gland after vaginal infection of mice.
1996
After vaginal infections of mice with neuroinvasive strains of herpes simplex virus type 1 and 2 (HSV-1, HSV-2) virus replicates in the epithelium of the vagina, in the paravaginal ganglia, in the spinal cord and finally in the brain and in the adrenal glands. However, viral antigens could be demonstrated only in the medulla of the adrenal glands but not in the cortex, as assessed by immunohistochemistry (IHC). HSV could not be isolated from liver, spleen, uterus, and ovaries. This contrasts to the intraperitoneal (i.p) route of infection with replication in different visceral organs including the adrenal gland's cortex.
Rapid and sensitive detection of metapneumovirus in clinical specimens by indirect fluorescence assay using a monoclonal antibody.
2008
Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of hMPV and to determine its epidemiology. Respiratory samples from 1,386 patients collected during 2 consecutive years were screened for hMPV using indirect immunofluorescence (IFA) assay with a monoclonal antibody. Forty-three patients tested positive for hMPV by the IFA method. In parallel, the samples were examined with RT-PCR on the F gene. Of these, 41 specimens were RT-PCR p…
Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes.
2002
<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B virus (HBV) surface (HBs) and core (HBc) antigens, and RNA phage fr coats (frCP). <i>Methods:</i> The profile of immunoglobulin isotypes induced in Balb/C mice after immunization with purified chimeric proteins was studied. <i>Results:</i> The HBs*-E7(35–54) protein expressing E7 residues 35–54 between residues 139 and 142 of the HBs carrier formed HBs-like particles…
Enhancement by TNF-alpha of reactivation and replication of latent herpes simplex virus from trigeminal ganglia of mice.
1995
The influence of tumor-necrosis-factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukine-1 (IL-1) and IL-3 on the in vitro reactivation frequency and replication rate of trigeminal ganglia of mice latently infected with herpes simplex virus (HSV) strain KOS was studied. It could be demonstrated that TNF-alpha and possibility GM-CSF, but not IL-1 and IL-3, enhanced the reactivation frequency and replication of HSV. Interferon alpha/beta (IFN alpha/beta) prevented reactivation and replication.
Procedures for study of teaching practices. Literature Review
2012
Interest in studying teaching practices has increased, because of the need to evaluate teachers and dissatisfaction with the usual ways of doing it. Recent approaches with Value-Added Models, based on students’ results on achievement tests do not seem satisfactory. The article is based on a review of literature and classifies the approaches to practices in three groups: instruments based on information given by teachers; observation protocols; and approaches based on the analysis of products of the practices. Specific tools are described and advantages and disadvantages of the three approaches are discussed. El interés por las prácticas docentes ha aumentado, en parte por la necesidad de ev…
Musiikillisen vuorovaikutuksen dynamiikkaa : tapaustutkimus musiikkiterapiassa soitetuista improvisaatioista
2012
Tässä kandidaatin tutkielmassa tutkitaan psykodynaamisen musiikkiterapiajakson aikana soitettuja improvisaatioita musiikkiterapeuttiseen käyttöön kehiteltyjen musiikkianalyysimenetelmin. Tutkimuksen tarkoituksena on tarkastella asiakkaan ja terapeutin välistä musiikillista vuorovaikutusta, musiikin dynamiikkaa ja siinä tapahtuvia muutoksia terapian kaaressa. Tutkimuksen aineisto on osa Jyväskylän yliopiston musiikin laitoksella tehtyä kansainvälistä Braintuning- tutkimusta, jossa selvitettiin improvisaatiolle pohjautuvan musiikkiterapian vaikutusta masennuksesta toipumiseen. Tämän tutkimuksen aineisto koostuu yhden asiakkaan musiikkiterapiassa soitettujen improvisaatioiden äänitteistä. Musi…
Effect of temperature on anaerobic treatment of black water in UASB-septic tank systems
2007
The effect of northern European seasonal temperature changes and low temperature on the performance of upflow anaerobic sludge blanket (UASB)-septic tanks treating black water was studied. Three UASB-septic tanks were monitored with different operational parameters and at different temperatures. The results indicated the feasibility of the UASB-septic tank for (pre)treatment of black water at low temperatures with respect to removal of suspended solids and dissolved organic material. Inoculum sludge had little effect on COD(ss) removal, though in the start-up phase some poorly adapted inoculum disintegrated and washed out, thus requiring consideration when designing the process. Removal of …
The sustainable lifestyle of Aalborg
2020
The strongest and most appealing image of Aalborg is its waterfront, facing the Limfjord that runs across Denmark, halfway between Kattegat and the North Sea. However, until a few decades ago, this water-“front” could have been described more likely as a sequence of “rears” of inaccessible, degraded and disused factories and abandoned port infrastructures. Fed by the strongest will power, the city council decided to adopt a new project in order to emerge from the economic crisis that, between the Seventies and Eighties, saw factories and industries shutting down one by one. The idea therefore was to reconvert the image of the city from an industrial and port center into a strategic, innovat…