Search results for "LC"
showing 10 items of 31477 documents
Transcriptome Response of Metallicolous and a Non-Metallicolous Ecotypes of Noccaea goesingensis to Nickel Excess
2020
Root transcriptomic profile was comparatively studied in a serpentine (TM) and a non-metallicolous (NTM) population of Noccaea goesingensis in order to investigate possible features of Ni hyperaccumulation. Both populations were characterised by contrasting Ni tolerance and accumulation capacity. The growth of the TM population was unaffected by metal excess, while the shoot biomass production in the NTM population was significantly lower in the presence of Ni in the culture medium. Nickel concentration was nearly six- and two-fold higher in the shoots than in the roots of the TM and NTM population, respectively. The comparison of root transcriptomes using the RNA-seq method indicated disti…
Current Experience with Application of Metal-based Nanofertilizers
2019
Agriculture is one of the many fields in which nanotechnology is currently applied. At the nano-scale, materials exhibit different properties mainly due to the reduced molecular size which allows different interactions between molecules. Nowadays, the agricultural sector demands methods that not only increase crop productivity, but are also sustainable and produce less environmental impact. Large-scale application of chemical fertilizers is common in farming with the aim of increasing productivity. The use of large doses of fertilizers, however, causes more harm than good. Chemically intensive agriculture disturbs the soil-mineral balance, pollutes soil, water and air, and makes lands less …
In-Field and Early Detection of Xylella fastidiosa Infections in Olive Using a Portable Instrument
2019
Xylella fastidiosa subsp. pauca (Xfp) is a gram-negative pathogenic bacteria responsible for serious diseases (Purcell, 2013) that inflicts considerable economic loss (Li et al., 2007; Luvisi et al., 2017). The pathogen has been linked to olive quick decline syndrome (OQDS). This devastating olive disease was first observed in Salento (Apulia, southeastern Italy) in 2009. Infected trees respond to Xfp infection with scattered desiccation of twigs and small branches in the upper crown, which extend to the rest of the canopy, showing the characteristic blight effect. The disease causes tree death within a few years from the onset of symptoms (Martelli, 2016). The primary agronomic procedure f…
Synthesis of Isomaltooligosaccharides by Saccharomyces cerevisiae Cells Expressing Aspergillus niger α‑Glucosidase
2017
The α-glucosidase encoded by the aglA gene of Aspergillus niger is a secreted enzyme belonging to family 31 of glycoside hydrolases. This enzyme has a retaining mechanism of action and displays transglycosylating activity that makes it amenable to be used for the synthesis of isomaltooligosaccharides (IMOs). We have expressed the aglA gene in Saccharomyces cerevisiae under control of a galactose-inducible promoter. Recombinant yeast cells expressing the aglA gene produced extracellular α-glucosidase activity about half of which appeared cell bound whereas the other half was released into the culture medium. With maltose as the substrate, panose is the main transglycosylation product after 8…
Evolution of the Dentition in Holocephalans (Chondrichthyes) Through Tissue Disparity
2020
Abstract The Holocephali is a major group of chondrichthyan fishes, the sister taxon to the sharks and rays (Elasmobranchii). However, the dentition of extant holocephalans is very different from that of the elasmobranchs, lacking individual tooth renewal, but comprising dental plates made entirely of self-renewing dentine. This renewal of all tissues occurs at the postero-lingual plate surface, as a function of their statodont condition. The fossil record of the holocephalans illuminates multiple different trends in the dentition, including shark-like teeth through to those with dentitions completely lacking individual teeth. Different taxa illustrate developmental retention of teeth but w…
Identification of ABA-Mediated Genetic and Metabolic Responses to Soil Flooding in Tomato (
2021
Soil flooding is a compound abiotic stress that alters soil properties and limits atmospheric gas diffusion (O2 and CO2) to the roots. The involvement of abscisic acid (ABA) in the regulation of soil flooding-specific genetic and metabolic responses has been scarcely studied despite its key importance as regulator in other abiotic stress conditions. To attain this objective, wild type and ABA-deficient tomatoes were subjected to short-term (24 h) soil waterlogging. After this period, gas exchange parameters were reduced in the wild type but not in ABA-deficient plants that always had higher E and gs. Transcript and metabolite alterations were more intense in waterlogged tissues, with genoty…
What has been thought and taught on the lunar influence on plants in agriculture? Perspective from physics and biology
2020
This paper reviews the beliefs which drive some agricultural sectors to consider the lunar influence as either a stress or a beneficial factor when it comes to organizing their tasks. To address the link between lunar phases and agriculture from a scientific perspective, we conducted a review of textbooks and monographs used to teach agronomy, botany, horticulture and plant physiology; we also consider the physics that address the e ects of the Moon on our planet. Finally, we review the scientific literature on plant development, specifically searching for any direct or indirect reference to the influence of the Moon on plant physiology. We found that there is no reliable, science-based evi…
Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives
2019
Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advan…
In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System
2019
Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38°
The evolution of dwarf shrubs in alpine environments: a case study ofAlchemillain Africa
2015
BACKGROUND AND AIMS Alpine and arctic environments worldwide, including high mountains, are dominated by short-stature woody plants (dwarf shrubs). This conspicuous life form asserts considerable influence on local environmental conditions above the treeline, creating its own microhabitat. This study reconstructs the evolution of dwarf shrubs in Alchemilla in the African tropical alpine environment, where they represent one of the largest clades and are among the most common and abundant plants. METHODS Different phylogenetic inference methods were used with plastid and nuclear DNA sequence markers, molecular dating (BEAST and RelTime), analyses of diversification rate shifts (MEDUSA and BA…