Search results for "LET"
showing 10 items of 23489 documents
Sic astra mereri. Un’analisi della Controversia de statua viri fortis di Draconzio
2017
Nel contesto dell’Africa vandalica del quinto secolo si distingue una singolare reviviscenza degli studi retorici e, segnatamente, declamatori. Sotto questo profilo, emerge con particolare rilievo uno dei Romulea di Draconzio; si tratta del numero 5 della raccolta, dal titolo Controversia de statua viri fortis. Nel componimento, una esercitazione retorica elaborata in versi esametrici, l’autore riattualizza un motivo topico delle scuole di declamazione, il contrasto tra un dives ed un pauper, arricchendolo di molteplici stratificazioni letterarie, da Virgilio a Lucano soprattutto, che tuttavia non celano lo straordinario interesse draconziano ad intestarsi un certo aggiornamento delle prati…
Tra calunnia e inganno: strategia della parola nella Fedra di Seneca
2013
Il comportamento comunicativo di Fedra crea un corto circuito da cui si sprigiona il pathos del dramma senecano. Nel tessuto tragico confluiscono infatti aspetti della comunicazione quali la manipolazione, l’inganno e la calunnia che mediano il trapasso fra passione e menzogna degradando il portato metaforico della protagonista. Nucleo e vettore di propulsione della struttura drammatica della Phaedra di Seneca è infatti la potenza della parola che svela e nasconde fra trasparenza e indecifrabilità sottraendosi alla chiarezza della verità per farsi sottile strumento di menzogna. Phedra's communicative behaviour creates a short circuit from which the pathos of Seneca drama emanates through so…
Il colore e l'ombra. Aspetti della posterità ciceroniana
2010
Agrestis cum erudito: scenografie del discorso nell’Apologia di Apuleio
2017
In Apologia Apuleius’s speech shows a rich ‘geography’: private homes, shops, villas and inns are the ingredients of a rhetorical strategy aimed at emphasizing the cultural and ethical distance between accusation and defense. An important role is played by the topical contrast between the country and the city, used to make a strong impact on the public and on the judge and to dismantle and discredit 'a priori' the accusations. In Apologia, therefore, the places are not only the background to the trial but are also conceived as a scenery of the word and as a ‘visual’ instrument to outline the parties involved.
Etanercept maintains the clinical benefit achieved by infliximab in patients with rheumatoid arthritis who discontinued infliximab because of side ef…
2007
Objective: To evaluate the efficacy of switching to etanercept treatment in patients with rheumatoid arthritis who already responded to infliximab, but presented side effects. Methods: Charts of 553 patients with rheumatoid arthritis were retrospectively reviewed to select patients who responded to the treatment with infliximab and switched to etanercept because of occurrence of adverse effects. Clinical data were gathered during 24 weeks of etanercept treatment and for the same period of infliximab treatment before infliximab was stopped. Disease Activity Score computed on 44 joints (DAS-44), erythrocyte sedimentation rate (ESR) 1st hour, Visual Analogue Scale (VAS) of pain, Health Assessm…
Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin s…
1994
Cytotoxic necrotizing factor type 2 (CNF2) produced by Escherichia coli strains isolated from intestinal and extraintestinal infections is a dermonecrotic toxin of 110 kDa. We cloned the CNF2 gene from a large plasmid carried by an Escherichia coli strain isolated from a lamb with septicemia. Hydropathy analysis of the deduced amino acid sequence revealed a largely hydrophilic protein with two potential hydrophobic transmembrane domains. The N-terminal half of CNF2 showed striking homology (27% identity and 80% conserved residues) to the N-terminal portion of Pasteurella multocida toxin. Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol…
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
2001
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…
Mtlinteracts with members of Egfr signaling and cell adhesion genes in the Drosophila eye
2011
Mtl is a member of the Rho family of small GTPases in Drosophila. It was shown that Mtl is involved in planar cell polarity (PCP) establishment, together with other members of the same family like Cdc42, Rac1, Rac2 and RhoA. However, while Rac1, Rac2 and RhoA function downstream of Dsh in Fz/PCP signaling and upstream of a JNK cassette, Mtl and Cdc42 do not. To determine the functional context of Mtl during PCP establishment in the Drosophila eye, we performed a loss-of-function screen to search for dominant modifiers of a sev>Mtl rough eye phenotype. In addition, genetic interaction assays with candidate genes were also carried out. Our results show that Mtl interacts genetically with memb…
Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in …
2010
The organization and dynamics of microtubules (MTs) and the actin cytoskeleton are critical for the correct development and functions of neurons, including intracellular traffic and signaling. In vitro ethanol exposure impairs endocytosis, exocytosis, and nucleocytoplasmic traffic in astrocytes and alters endocytosis in cultured neurons. In astrocytes, these effects relate to changes in the organization and/or function of MTs and the actin cytoskeleton. To evaluate this possibility in hippocampal cultured neurons, we analyzed if chronic ethanol exposure affects the levels, assembly, and cellular organization of both cytoskeleton elements and the possible underlying mechanisms of these effec…
Muscle and serum metabolomes are dysregulated in colon-26 tumor-bearing mice despite amelioration of cachexia with activin receptor type 2B ligand bl…
2019
Cancer-associated cachexia reduces survival, which has been attenuated by blocking the activin receptor type 2B (ACVR2B) ligands in mice. The purpose of this study was to unravel the underlying physiology and novel cachexia biomarkers by use of the colon-26 (C26) carcinoma model of cancer cachexia. Male BALB/c mice were subcutaneously inoculated with C26 cancer cells or vehicle control. Tumor-bearing mice were treated with vehicle (C26+PBS) or soluble ACVR2B either before (C26+sACVR/b) or before and after (C26+sACVR/c) tumor formation. Skeletal muscle and serum metabolomics analysis was conducted by gas chromatography-mass spectrometry. Cancer altered various biologically functional groups …