Search results for "LIBRARY"
showing 10 items of 3069 documents
cDNA Synthesis and Cloning
1998
The isolation of intact messenger RNA and its conversion into cDNA copies by avian or Moloney murine reverse transcriptase, as well as subsequent amplification of gene transcripts by the PCR technique, are becoming increasingly important tools in molecular biology. At present, these techniques have been often necessary and widely used for the analysis of individual mRNA levels in cells and tissues by Northern blot analysis, nuclease protection analysis and in situ hybridization. Another important application of RNA templates is the construction of representative cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukary…
Identification of Tumor-Associated Autoantigens With SEREX
2004
Serological analysis of tumor antigens by recombinant cDNA expression cloning (SEREX) allows the systematic cloning of tumor antigens recognized by the spontaneous autoantibody repertoire of cancer patients. For SEREX, cDNA expression libraries are constructed from fresh tumor specimens, packaged into lambda-phage vectors, and expressed recombinantly in Escherichia coli. Recombinant proteins expressed during the lytic infection of bacteria are transferred onto nitrocellulose membranes to be probed with diluted autologous patient serum for identification of clones reactive with high-titered IgG antibodies. This chapter describes the SEREX technology in detail.
Cloning and Characterization of Overlapping DNA Fragments of the Toxin A Gene of Clostridium difficile
1989
Clostridium difficile, a human pathogen, produces two very large protein toxins, A and B (250-600 kDa), which resist dissociation into subunits. To clone the toxin A gene, a genomic library of 3-8 kb chromosomal DNA fragments of C. difficile strain VPI 10463 established in pUC12 was screened with a rabbit polyclonal toxin A antiserum. Thirty-five clones were isolated which carried 2.5-7.0 kb inserts representing a 10 kb region of the C. difficile genome. All the inserts were oriented in the same direction, suggesting that toxin A gene expression was under control of the lac promoter of the pUC12 vector. Western blot experiments revealed the presence of low amounts of fusion proteins of vari…
Sequencing and analysis of the gene encoding the α-toxin of Clostridium novyi proves its homology to toxins A and B of Clostridium difficile
1995
A library of total Clostridium novyi DNA was established and screened for the alpha-toxin gene (tcn alpha) by hybridization with oligonucleotides derived from a partial N-terminal sequence and by using specific antisera. Overlapping subgenic tcn alpha fragments were isolated and subsequently the total sequence of tcn alpha was determined. The 6534 nucleotide open reading frame encodes a polypeptide of M(r) 250,166 and pI 5.9. The N-terminal alpha-toxin (Tcn alpha) sequence MLITREQLMKIASIP determined by Edman degradation confirmed the identity of the reading frame and the assignment of the translation start point. The toxin is not modified posttranslationally at its N-terminus nor does it co…
A Literature Review on Cloud Computing Adoption Issues in Enterprises
2014
Part 3: Creating Value through Applications; International audience; Cloud computing has received increasing interest from enterprises since its inception. With its innovative information technology (IT) services delivery model, cloud computing could add technical and strategic business value to enterprises. However, cloud computing poses highly concerning internal (e.g., Top management and experience) and external issues (e.g., regulations and standards). This paper presents a systematic literature review to explore the current key issues related to cloud computing adoption. This is achieved by reviewing 51 articles published about cloud computing adoption. Using the grounded theory approa…
Les manuscrits de l’époque romane dans les listes de livres des établissements bénédictins bourguignons (XIe-XIVe siècle)
2017
International audience
Co-construct territorial cultures
2015
International audience; Carrying out investigations in the framework of the scientific network INTI (International Network of Territorial Intelligence, http://inti.hypotheses.org/), the main idea is here to value the contribution of multiple approaches in territorial information and communication science that can be experienced there. Inspired by the triad of Pestalozzi, these experiences have applied and adapted the territorial engineering approach "Catalyse" in their territories.From the cooperative structuring of information to concrete initiatives of local change, communication processes make possible iterations between understanding and action, in which the analysis of the effects of s…
Querytogether : Enabling entity-centric exploration in multi-device collaborative search
2018
Collaborative and co-located information access is becoming increasingly common. However, fairly little attention has been devoted to the design of ubiquitous computing approaches for spontaneous exploration of large information spaces enabling co-located collaboration. We investigate whether an entity-based user interface provides a solution to support co-located search on heterogeneous devices. We present the design and implementation of QueryTogether, a multi-device collaborative search tool through which entities such as people, documents, and keywords can be used to compose queries that can be shared to a public screen or specific users with easy touch enabled interaction. We conducted…
Comparison of Metrics for the Classification of Soils Under Variable Geometrical Conditions Using Hyperspectral Data
2008
International audience; The objective of this letter is to find a distance metric between reflectance spectra that is not sensitive to the variations on the soil reflectance induced by the geometry of solar-view angles. This is motivated by the fact that differences between spectra measured for the same soil under different observation and illumination configurations can lead to misclassifications. Using 26 soils of different compositions simulated with Hapke’s model and 92 soils of different compositions measured under 28 solarview angle geometries in laboratory conditions, we tested three metrics, namely, root-mean-square error, spectral angle mapper, and R2 (the coefficient of determinat…
Collaborative writing with web 2.0 technologies: education students' perceptions
2011
Published version of an article from the journal: Journal of Information Technology Education. Also available from the publisher: http://jite.org/documents/Vol10/JITEv10IIPp073-103Brodahl948.pdf Executive Summary Web 2.0 technologies are becoming popular in teaching and learning environments. Among them several online collaborative writing tools, like wikis and blogs, have been integrated into educational settings. Research has been carried out on a wide range of subjects related to wikis, while other, comparable tools like Google Docs and EtherPad remain largely unexplored in the literature. This work presents a case study investigating education students' perceptions of collaborative writ…