Search results for "Labeling"
showing 10 items of 372 documents
Metalloprotease meprin beta generates nontoxic N-terminal amyloid precursor protein fragments in vivo.
2011
Identification of physiologically relevant substrates is still the most challenging part in protease research for understanding the biological activity of these enzymes. The zinc-dependent metalloprotease meprin β is known to be expressed in many tissues with functions in health and disease. Here, we demonstrate unique interactions between meprin β and the amyloid precursor protein (APP). Although APP is intensively studied as a ubiquitously expressed cell surface protein, which is involved in Alzheimer disease, its precise physiological role and relevance remain elusive. Based on a novel proteomics technique termed terminal amine isotopic labeling of substrates (TAILS), APP was identified …
Novel Combination of Sorafenib and Celecoxib Provides Synergistic Anti-Proliferative and Pro-Apoptotic Effects in Human Liver Cancer Cells
2013
Molecular targeted therapy has shown promise as a treatment for advanced hepatocellular carcinoma (HCC). Sorafenib, a multikinase inhibitor, recently received FDA approval for the treatment of advanced HCC. However, although sorafenib is well tolerated, concern for its safety has been expressed. Celecoxib (Celebrex®) is a selective cyclooxygenase-2 (COX-2) inhibitor which exhibits antitumor effects in human HCC cells. The present study examined the interaction between celecoxib and sorafenib in two human liver tumor cell lines HepG2 and Huh7. Our data showed that each inhibitor alone reduced cell growth and the combination of celecoxib with sorafenib synergistically inhibited cell growth an…
Osteogenic differentiation of periodontal fibroblasts is dependent on the strength of mechanical strain
2012
Abstract Objective During orthodontic therapy the correct strength of mechanical strain plays a key role for bone remodelling during tooth movement. Aim of this study was to investigate the osteogenic differentiation of human periodontal ligament fibroblasts (HPdLF) depending on the applied strength of mechanical strain compared to osteoblasts (HOB). Design HPdLF and HOB were loaded with different strengths (1%, 5% and 10%) of static mechanical strain (SMS) for 12 h in vitro. Viability was verified by MTT and apoptosis by TUNEL assay. Gene expression of cyclin D1, collagen type-1 (COL-I), alkaline phosphatase (ALP), osteocalcin, osteoprotegerin (OPG) and receptor activator of the NF-κB liga…
Cells of extramammary Paget's disease express cytokeratins different from those of epidermal cells.
1985
The patterns of expression of cytokeratin polypeptides which are closely correlated to routes of differentiation of epithelial cells were studied in extramammary Paget's disease. Cytokeratins of uninvolved and involved epidermis were analyzed by two-dimensional gel electrophoresis of microdissected tissue preparations as well as by immunofluorescence microscopy using cytokeratin antibodies with different specificities. In uninvolved epidermis, cytokeratins Nos. 1, 5, 6, 10, 11, 14, and 16, characteristic of keratinocytes, were found. Epidermis infiltrated by Paget's cells contained the same components and, in addition, cytokeratins Nos. 7, 8, 18, and 19, the latter being characteristic of s…
Reliability of adapted version of Italian Label tobacco Impact Index for the adolescent: ALII
2017
OBJECTIVE: The aim of this study is to assess the reliability of the Adolescent Label Impact Index (ALII) , it is an adolescent adapted version of Italian LII of the tobacco products warnings. MATERIAL AND METHODS: A sample including students aged 13-15 years was considered. The ALII is constructed by 4 items: salience, harm, quitting and forgo. The questionnaire was self-administered to study participants twice with 3 days between each administration (T1 and T2) to measure reliability. The internal consistency using Cronbach's alpha and Corrected Item-Total Correlations (CITC) and the test-retest reliability applying Pearson's correlation were computed. RESULTS: Cronbach's alpha ranges fro…
Radiolabeling of Nanoparticles and Polymers for PET Imaging
2014
Nanomedicine has become an emerging field in imaging and therapy of malignancies. Nanodimensional drug delivery systems have already been used in the clinic, as carriers for sensitive chemotherapeutics or highly toxic substances. In addition, those nanodimensional structures are further able to carry and deliver radionuclides. In the development process, non-invasive imaging by means of positron emission tomography (PET) represents an ideal tool for investigations of pharmacological profiles and to find the optimal nanodimensional architecture of the aimed-at drug delivery system. Furthermore, in a personalized therapy approach, molecular imaging modalities are essential for patient screeni…
Devianza, problemi sociali e forme di controllo
2019
Dalle etnografie del bere in Polinesia allo studio transculturale della balbuzie, dalla paranoia come effetto delle dinamiche di esclusione alle etichette devianti elaborate dalle agenzie del controllo sociale e alle conseguenze dello stigma sulla riorganizzazione simbolica del sé “deviante”, questo classico di Edwin M. Lemert è tra i primi studi ad allontanarsi dall’atteggiamento teorico secondo il quale è la devianza a dar luogo al controllo sociale. Per il sociologo americano è invece il controllo a produrre la devianza e a far sorgere ulteriori forme di controllo sempre più invasive. Devianza, problemi sociali e forme di controllo costituisce ancora oggi un testo di riferimento per gli …
Cognitive Linguistics as the Underlying Framework for Semantic Annotation
2012
In recent years many attempts have been made to design suitable sets of rules aimed at extracting the semantic meaning from plain text, and to achieve annotation, but very few approaches make extensive use of grammars. Current systems are mainly focused on extracting the semantic role of the entities described in the text. This approach has limitations: in such applications the semantic role is conceived merely as the meaning of the involved entities without considering their context. As an example, current semantic annotators often specify a date entity without any annotation regarding the kind of the date itself i.e. a birth date, a book publication date, and so on. Moreover, these system…
Introduzione. Anticonformismo, eclettismo e innovazione nell’opera di Edwin M. Lemert
2021
Il saggio introduce lo sviluppo intellettuale e le opere tradotte per la prima volta in lingua italiana del sociologo Edwin M. Lemert, facendo riferimento soprattutto all'ultima fase della sua produzione e ai primi lavori inesistenti in lingua italiana The essay introduces the intellectual development and the works translated for the first time into Italian by the sociologist Edwin M. Lemert, referring above all to the last phase of his production and to the early non-existent works in Italian.
Una teoria generale del comportamento sociopatico
2021
General theory of behavior sociopath.