Search results for "Lactate"

Molecular Evolution of Lactate Dehydrogenase in the Developing Nervous Tissue*

1964

Publisher Summary This chapter describes both electrophoretic and catalytic studies on lactate dehydrogenase (LDH) in the developing brain. It has been found that the enzyme exhibits at birth a composition that gradually changes to attain the final pattern observed in the adult. The neurochemical findings on LDH isozymes parallel the rapid growth in the first days after birth. Isozyme E, which is not present in detectable amounts 2 days after birth, represents 15% of the enzyme on the 22nd day. Similarly, the percentage decrease of isozyme B is quite steep at the beginning. Nevertheless, the final pattern is attained only at the end of the 2nd month that is, when the cerebral cortex is full…

REGIONAL, COMPARATIVE AND ONTOGENETIC STUDIES ON LACTATE DEHYDROGENASE OF NERVOUS TISSUE

1962

Energy Metabolism Characterization of a Novel Cancer Stem Cell-Like Line 3AB-OS

2013

Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutic target, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells, exhibit a reduced mitochondrial activity and a stronger dependence on aerobic glycolysis. Here, we aimed to comparatively analyze bioenergetics features of the human osteosarcoma 3AB-OS CSC-like line, and the parental osteosarcoma MG63 cells, from which 3AB-OS cells have been previously selected. Our results suggest that 3AB-OS cells depend on glycolytic metabolism more strongly than MG63 cells. Indeed, growth in…

Combined strength and endurance exercise induced fatigue and recovery

2012

Muscular fatigue is usually defined as the inability to maintain a given exercise intensity or adequate muscle force and therefore maximal isometric force, neural activation and fast force production capacity have often used determinants of acute fatigue. The purpose of the present study was to measure the acute effects of combined strength and endurance exercise on force production, muscle activation and work economy. In addition this study investigated the rate of recovery comparing the active and passive recovery models and the association of the initial fitness levels of subjects. 16 (27 ± 4 years old) male reservists performed combined strength (5x10x70%RM leg-press) and endurance (5x1…

Investigations of methyl lactate in the presence of reverse micelles by vibrational spectroscopy and circular dichroism

2012

Abstract The FT-IR and vibrational circular dichroism (VCD) spectra of ( S )- and -( R )-methyl lactate have been recorded for neat samples and at various concentrations in CCl 4 and DMSO solutions. These spectra are used to analyse the FT-IR and VCD spectra of methyl lactates in presence of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in CCl 4 , where the surfactant molecules are known to form reverse micelles. Some tendency of methyl lactate to interact with AOT micellar aggregates is observed, but not as well defined as previously observed for dimethyl tartrate in analogous circumstances. Besides, near infrared (NIR) absorption and VCD data have been obtained for most of the above system…

Prognostic factors for non-asphyxia-related cardiac arrest patients undergoing extracorporeal rewarming - HELP Registry Study

2020

Objective: Extracorporeal rewarming is the treatment of choice for patients who had hypothermic cardiac arrest, allowing for best neurologic outcome. The authors’ goal was to identify factors associated with survival in nonasphyxia-related hypothermic cardiac arrest patients undergoing extracorporeal rewarming. Design: All 38 cardiac surgery departments in Poland were encouraged to report consecutive hypothermic cardiac arrest patients treated with extracorporeal life support. All variables collected were analyzed in order to compare survivor and nonsurvivor groups. The parameters available at the initiation of extracorporeal rewarming were considered as potential predictors of survival in …

The purpose of lactate level detection in migraine and fibromyalgia

not available

Effects of cyclic fatty acid monomers on the function of cultured rat cardiac myocytes in normoxia and hypoxia

1992

Abstract It was shown in a previous study that rat ventricular myocytes in culture do incorporate in their phospholipids the cyclic fatty acid monomers (CFAM), produced from linolenic acid by heating linseed oil. In the present study, the functional consequences of this incorporation were investigated. As compared to control cells, CFAM containing cells displayed altered electromechanical properties evidenced by decreased maximal diastolic potential, upstroke velocity and rate and a shortened action potential duration. Moreover, oxygen depletion exerted much more adverse effects on CFAM-treated cells than in control cells, such as spontaneous rhythm abnormalities, cell shortening failure an…

SOME COMPARATIVE REMARKS ON THE TRANSIENT CHANGE IN LACTIC ACID CONTENT IN SEA URCHIN EGGS FOLLOWING FERTILIZATION.

1964

Abstract During the first few minutes following fertilization a transient increase in the concentration of lactic acid occurs in the eggs of Arbacia lixula , whereas no change at all is observed in the eggs of Paracentrotus liuidus . In the eggs of Psammechinus miliaris there is a transient decrease, soon followed by a recovery so that the level of the unfertilized egg is again reached.

The role of lactate besides the lactic acidosis

2013

Lactic acidosis (LA) is the most common form of metabolic acidosis defined by values of lactate greater than 5 mmol / l and by a pH <7.34. The pathogenesis of LA involves hypoxic (type A) and non hypoxic (type B) causes which are often coexisting. Lactic acidosis is usual in hospitalized population especially in subjects in intensive care units, in which lactate levels on admission could be predictors of mortality even in the absence of organ dysfunction or shock. The outcome is mainly dependent on the cardiovascular effects of acidosis. In subjects with cardiogenic shock, the increased lactate/pyruvate ratio, detectable at onset, is correladed with mortality. An early assessment of blood a…