Search results for "Lament"
showing 10 items of 895 documents
Immunodetection of the microvillous cytoskeleton molecules villin and ezrin in the parasitophorous vacuole wall of Cryptosporidium parvum (Protozoa: …
1999
Microvilli - actin - villin - ezrin - Cryptosporidium parvum The sporozoites and merozoites of the Apicomplexan protozoan Cryptosporidium parvum (C. parvum) invade the apical side of enterocytes and induce the formation of a parasitophorous vacuole which stays in the brush border area and disturbs the distribution of microvilli. The vacuole is separated from the apical cytoplasm of the cell by an electron-dense layer of undetermined composition. In order to characterize the enterocyte cytoskeleton changes that occur during C. parvum invasion and development, we used both confocal immunofluorescence and immunoelectron microscopy to examine at the C.parvum-enterocyte interface the distributio…
Flexible ferromagnetic filaments and the interface with biology
2009
Flexible ferromagnetic filaments are studied both theoretically and experimentally. Two main deformation modes of the filament at magnetic field inversion are theoretically described and observed experimentally by using DNA-linked chains of ferromagnetic particles. Anomalous orientation of ferromagnetic filaments perpendicular to AC field with a frequency which is high enough is predicted and confirmed experimentally. By experimental studies of magnetotactic bacteria it is demonstrated how these properties of ferromagnetic filaments may be used to measure the flexibility of the chain of magnetosomes.
The FBN2 gene: new mutations, locus-specific database (Universal Mutation Database FBN2), and genotype-phenotype correlations.
2009
International audience; Congenital contractural arachnodactyly (CCA) is an extremely rare disease, due to mutations in the FBN2 gene encoding fibrillin-2. Another member of the fibrillin family, the FBN1 gene, is involved in a broad phenotypic continuum of connective-tissue disorders including Marfan syndrome. Identifying not only what is in common but also what differentiates these two proteins should enable us to better comprehend their respective functions and better understand the multitude of diseases in which these two genes are involved. In 1995 we created a locus-specific database (LSDB) for FBN1 mutations with the Universal Mutation Database (UMD) tool. To facilitate comparison of …
Mechanical testing and numerical modelling of pull-wound carbon-epoxy spinnaker poles
2002
The paper deals with experimental testing and numerical simulation of the mechanical behaviour of multi-layer cylindrical coupons, of two different diameters, made in carbon-epoxy composite. The aim of the study is to provide a simple and effective numerical model that can be used as a design tool for structural elements having analogous geometrical and manufacturing characteristics. The numerical analysis, performed in the elastic regime with a standard finite element (FE) code, was strongly correlated with the laboratory determination of fibre-volume fractions and of some elastic parameters of the material system. Other parameters, like the shear modulus values G, were in fact appropriate…
Thermal and rheological characterisation of a thermoset matrix for filament winding modelling
2004
Rheo-kinetic behaviour of an epoxy resin, coupled with an aliphatic polyamines hardener, used in fibre reinforced plastics, was analysed comparing experimental data to theoretical models. Then the modelling of technological filament winding process for thermoset matrix composites, developed through a numerical code realized with MATLAB, is reported. The model includes winding and curing phase decoupling the manufacturing process into sub-models. Four sub-models are used: fibre motion, thermal, kinetic and rheological model. Considerable differences are obtained in process condition, using several thermal treatments. The numerical modelling helps to detect the process conditions to optimise…
Cultivable microorganisms associated with honeys of different geographical and botanical origin
2014
In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-IT…
Dynamic force sensing of filamin revealed in single-molecule experiments
2012
Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The cytoskeletal protein filamin is a key connecting element between the cytoskeleton and transmembrane complexes such as integrins or the von Willebrand receptor glycoprotein Ib. Here, we show using single-molecule mechanical measurements that the recently reported Ig domain pair 20–21 of human filamin A acts as an autoinhibited force-activatable mechanosensor. We developed a mechanical single-molecule competition assay that allows online observation of binding events of target peptides in solution to the strained domain pair. We find that fi…
Deformation of flexible ferromagnetic filaments under a rotating magnetic field
2020
This repository contains experimental data and images related to the publication: A. Zaben, G. Kitenbergs, A. Cēbers (2020) Deformation of flexible ferromagnetic filaments under a rotating magnetic field. Journal of Magnetism and Magnetic Materials, 499, 166233 https://doi.org/10.1016/j.jmmm.2019.166233 / https://arxiv.org/abs/1908.02604. Excel files are results named corresponding to figure number in the publication. Root file '1' is for experimental images used for Fig.3, 4 and 5; where either the length is constant having file names as the value of the field strength or named with length values with fixed field strength for different frequencies. The images are named as …
3D motion of flexible ferromagnetic filaments under rotating magnetic field
2020
This repository contains experimental data and numerical results related to the publication: A. Zaben, G. Kitenbergs, A. Cēbers (2020), 3D motion of flexible ferromagnetic filaments under rotating magnetic field. Soft Matter, https://doi.org/10.1039/D0SM00403K / https://arxiv.org/abs/2003.03737. Figs_data.xlsx contains the data presented in the figures. Experimental_Data.rar contains experimental images used to obtain the results for Fig. 3 and 9. The files are named with the operating frequency, field strength and filament length. Numerical.rar contains numerical results used in Fig.6, 8 and 9. The files are named with Cm values. The results are in .dat files named with Cm values follo…
Study of aerobic granular sludge stability in a continuous-flow membrane bioreactor.
2015
A granular continuous-flow membrane bioreactor with a novel hydrodynamic configuration was developed to evaluate the stability of aerobic granular sludge (AGS). Under continuous-flow operation (Period I), AGS rapidly lost their structural integrity resulting in loose and fluffy microbial aggregates in which filamentous bacteria were dominant. The intermittent feeding (Period II) allowed obtaining the succession of feast and famine conditions that favored the increase in AGS stability. Although no further breakage occurred, the formation of new granules was very limited, owing to the absence of the hydraulic selection pressure. These results noted the necessity to ensure, on the one hand the…