Search results for "Laser Scanning Microscopy"
showing 5 items of 25 documents
Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …
2015
ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…
Neue Erkenntnisse zur Ätiologie der sogenannten Strahlenkaries
1997
Fragestellung Die Atiologie der Strahlenkaries wird trotz vieler Untersuchungen kontrovers diskutiert: Die These einer direkten radiogenen Lasion des Zahnhartgewebes steht der einer indirekten Pathogenese uber die Radioxerostomie gegenuber.
Presence of endophytic bacteria in Vitis vinifera leaves as detected by Fluorescence in situ hybridization
2010
Fluorescence in situ hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM) was applied to detect and localize bacterial colonies in leaf tissues of Vitis vinifera. Leaves were cleared to minimize the autofluorescence of plant fragments. The use of fluorescently labeled bacterial probe EUB338 on discolored grapevine leaf disks allowed the estimation of the spatial distribution of different bacterial colonies. In particular, bacterial colonies were found in veins, cells, hairs, intercellular spaces, and in cut edges of leaf disks of both non-Acremonium byssoides-colonized and A. byssoides-colonized leaves of five different cultivars. Furthermore, CLSM confirmed th…
Effect of oxidoreduction potential and of gas bubbling on rheological properties and microstructure of acid skim milk gels acidified with glucono-del…
2009
Milk oxidoreduction potential was modified using gases during the production of a model dairy product and its effect on gel setting was studied. Acidification by glucono-delta-lactone was used to examine the physicochemistry of gelation and to avoid variations due to microorganisms sensitive to oxidoreduction potential. Four conditions of oxidoreduction potential were applied to milk: milk was gassed with air, nongassed, gassed with N(2), or gassed with N(2)H(2). The rheological properties and microstructure of these gels were determined using viscoelasticimetry, measurement of whey separation, and confocal laser scanning microscopy. It appeared that a reducing environment led to less-aggre…
Confocal laser scanning microscopy for the study of the morphological changes of the postextraction sites
2012
A better understanding of the remodeling process of postextraction sockets is essential in dental treatment planning. The aim of this study was to evaluate whether confocal laser scanning microscopy (CLSM) can be applied to imaging contour changes of postextraction sites, as well as to its quantification with image analysis of obtained three-dimensional images. This work describes a new application of the CLSM technique. The system used was the OLS3100-USS, LEXT model (Olympus((R))). CLSM was used for the surface analysis of the extraction site. The measurements taken with CLSM were: (1) mesio-distal distance, (2) alveolar ridge thickness, and (3) vestibular and lingual alveolar ridge heigh…