Search results for "Law"
showing 10 items of 23812 documents
Comment on “A simple way to incorporate uncertainty and risk into forest harvest scheduling”
2017
In a recent research article, Robinson et al. (2016) described a method of estimating uncertainty of harvesting outcomes by analyzing the historical yield to the associated prediction for a large number of harvest operations. We agree with this analysis, and consider it a useful tool to integrate estimates of uncertainty into the optimization process. The authors attempt to manage the risk using two different methods, based on deterministic integer linear programming. The first method focused on maximizing the 10th quantile of the distribution of predicted volume subject to area constraint, while the second method focused on minimizing the variation of total quantity of volume harvested sub…
Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine
2017
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…
Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D–cyclin-dependent kinase complexes
2017
Highlight Maize Kip-related proteins can be differentially phosphorylated by different cyclin D–cyclin-dependent kinase complexes and this influences their performance as cyclin-dependent kinase inhibitors.
Marine protected areas rescue a sexually selected trait in European lobster
2020
Abstract Marine protected areas (MPAs) are increasingly implemented worldwide to maintain and restore depleted populations. However, despite our knowledge on the myriad of positive responses to protection, there are few empirical studies on the ability to conserve species’ mating patterns and secondary sexual traits. In male European lobsters (Homarus gammarus), the size of claws relative to body size correlates positively with male mating success and is presumably under sexual selection. At the same time, an intensive trap fishery exerts selection against large claws in males. MPAs could therefore be expected to resolve these conflicting selective pressures and preserve males with large cl…
A self-inducible heterologous protein expression system in Escherichia coli
2016
AbstractEscherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter’s transcriptional activity. However, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture monitoring. Here, we report the novel SILEX (Self-InducibLe Expression) system, which is a convenient, cost-effective alternative that does not require cell density monitoring or IPTG inducti…
On the thermodynamic origin of metabolic scaling
2018
The origin and shape of metabolic scaling has been controversial since Kleiber found that basal metabolic rate of animals seemed to vary as a power law of their body mass with exponent 3/4, instead of 2/3, as a surface-to-volume argument predicts. The universality of exponent 3/4 -claimed in terms of the fractal properties of the nutrient network- has recently been challenged according to empirical evidence that observed a wealth of robust exponents deviating from 3/4. Here we present a conceptually simple thermodynamic framework, where the dependence of metabolic rate with body mass emerges from a trade-off between the energy dissipated as heat and the energy efficiently used by the organi…
The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the …
2017
In July 2016, an aggressive syndrome of tomato yellow leaf curl disease was reported in Sicily in tomato plants carrying the Ty-1 resistance gene. A total of 34 samples were collected and analyzed. Twenty-seven out of the 34 samples analyzed appeared to contain only recombinant molecules. One full sequence was obtained after cloning. Alignments and plot similarity analysis showed that the genome of the recombinant, named TYLCV-IL[IT:Sic23:16], was mostly derived from tomato yellow leaf curl virus (TYLCV), with a small region of 132 nucleotides in the non-coding region between the stem-loop and the start of the V2 ORF replaced by 124 nucleotides derived from a virus of a different species, t…
Effect of TiO2 nanoparticles in thyme under reduced irrigation conditions
2018
The nanotechnology is a relatively new technology that has recently entered the field of agriculture. Nanotechnology covers the integration or manipulation of individual atoms, molecules or molecular masses to a diverse array of structures allowing the production of new characteristics and traits of interest. The aim of this study was to evaluate the effects of foliar application of TiO2 nanoparticles on quantitative traits (plant height, number of branches, dry weight of shoots and roots) and the essential oil content of thyme under different levels of field capacity. Our results showed that the application of TiO2 nanoparticles had significant effects on thyme growth, while the essential …
Dynamics of Monoterpene Formation in Spike Lavender Plants
2017
The metabolic cross-talk between the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways was analyzed in spike lavender (Lavandula latifolia Med) on the basis of 13CO2-labelling experiments using wildtype and transgenic plants overexpressing the 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), the first and key enzyme of the MVA pathway. The plants were labelled in the presence of 13CO2 in a gas chamber for controlled pulse and chase periods of time. GC/MS and NMR analysis of 1,8-cineole and camphor, the major monoterpenes present in their essential oil, indicated that the C5-precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of both monoterpenes …
Essential Oil Yield, Chemical Composition, and Total Citral Content of Nine Cultivars of Cymbopogon Species from Western India
2016
ABSTRACTThe essential oil composition of five elite Cymbopogon varieties (OD-19, Pragati, RRL-16, NLG-84) and a hybrid (CPK-25) and four new selections (LS-1 to LS-4) were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The oil yield ranged from 0.31% to 1.0% with CPK-25 (1.0%) and LS-2 (0.95%) containing the highest. A total of 66 compounds, constituting 84.6–94.3% of the oils, were identified. The main compounds were geranial (29.9–41.6%), neral (25.8–32.5%), citronellol (0.3–12.8%), geranyl acetate (1.2–8.4%), and geraniol (1.8–8.2%). Of the cultivars, OD-19 (74.1%) had the highest and LS-3 (55.7%) contained the lowest amount of total citral. CKP-2…