Search results for "Lectin"

Uterine rejection after allogeneic uterus transplantation in the rat is effectively suppressed by tacrolimus

2013

Objective To evaluate the effects of the immunosuppressant tacrolimus on rejection of a transplanted uterus and on uterine expression of markers of inflammation and implantation. Design Experimental study. Setting University laboratory. Animal(s) Female rats. Intervention(s) Uteri from brown Norway rats were transplanted to Lewis rats, receiving either tacrolimus or no treatment. Sham groups underwent either hemihysterectomy or tacrolimus treatment. Main Outcome Measure(s) Gross morphology, histology, density of T-lymphocytes by immunohistochemistry, and mRNA levels of interleukin (IL)-1α, leukemia inhibitory factor (LIF), galectin-1, CD200, IL-15, interferon-inducible protein-10 (IP-10), a…

A lytic mechanism based on soluble phospholypases A2 (sPLA2) and b-galactoside specific lectins is exerted by Ciona intestinalis (ascidian) unilocula…

2011

Abstract Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca 2+ -dependent cytotoxic activity toward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocyte populations were separated (B1–B6 bands) through a Percoll discontinuous density gradient, the hemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS) were assayed. In addition the separated hemocytes were cultured and the cell-free culture medium (CFM) assayed after 3 h culture. Results support that unilocular refractile hemocytes (URGs), enriched in B5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysins can be released in…

P0493 : Selectin-targeting nanoparticles for immunomodulatory therapy of liver diseases

2015

Rilievo e ricostruzione virtuale del Ponte Sud di Hierapolis di Frigia (Turchia)

2015

[ES] El artículo se refiere al levantamiento topográfico y la reconstrucción en 3D de un puente-acueducto romano situado inmediatamente al sur de Hierápolis, en Frigia (suroeste de Turquía), a lo largo del antiguo camino dirigido a Colosas, en el interior de Anatolia. Actualmente sólo se conservan su pilar sur y escasos restos del pilar norte. Se encuentra en una situación muy difícil, en el interior de un valle estrecho y profundo, y nunca se ha estudiado antes. Durante la campaña de trabajo de campo de 2011 de la Misión Arqueológica Italiana, fue examinado con un diferencial de alta precisión del sistema GPS (para la documentación del plan y su posicionamiento en el mapa digital arqueológ…

Molecular arrangement between multivalent glycocluster andPseudomonas aeruginosaLecA (PA-IL) by atomic force microscopy: influence of the glycocluste…

2013

New therapeutics strategy against cystic fibrosis seeks to prevent the adhesion of the bacterium Pseudomonas aeruginosa (PA) on the epithelial cells in the lungs. One of the factors that induces the adhesion is the interaction between natural glycocluster present on the cells and lectins such as the PA lectin LecA (PA-IL) present on the bacterium. By introducing synthetic glycoclusters with a great affinity with the lectin PA-IL, the adhesion can be prevented. In this study, we characterized, by atomic force microscopy, the interaction between a tetra-galactosylated glycocluster and the PA-IL lectin for high concentration of lectins (2.5 μM).We showed that the strong lectin/lectin interacti…

Distribution patterns of neoglycoprotein-binding sites (endogenous lectins) and lectin-reactive glycoconjugates during cartilage and bone formation i…

1995

The distribution of endogenous lectins, visualized by labelled neoglycoproteins, and of defined oligosaccharide structures, reactive with plant lectins, during fetal development of the fingers was analyzed in sections of human 3- to 8-month-old fetal specimens. Chondrogenesis as well as ossification were correlated with characteristic modulations in the expression of both glycoligand-binding molecules and characteristic carbohydrate structures. Occurrence of xylose-specific receptors was judged to be an early sign of cartilage development. Similarly, α-mannosyl residues that had been attached to labelled carrier proteins were strongly bound by the extracellular matrix already during early s…

Differential specificity of substrate-attached lectins stimulating spreading of GH3-cells under serum-free, hormone-supplemented culture conditions

1982

Most mammalian cells are capable of growth in culture only when they are supplied with an appropriate substrate to which they can adhere and spread. To prepare suitable substrates different lectins were attached onto polystyrene tissue-culture dishes after coating with polylysine. GH3-cells (a pituitary-tumor-cell line) were seeded into the culture dishes containing serum-free, hormone-supplemented medium. When succinylated Concanavalin A (s-Con A), which binds specifically to mannose residues, is attached to the surface an extraordinary spreading of GH3-cells is induced within 15 to 20 min after seeding. Other lectins with a different sugar-binding specificity are less effective in inducin…

Origin of metazoan adhesion molecules and adhesion receptors as deduced from cDNA analyses in the marine sponge Geodia cydonium: a review.

1997

The phylogenetic relationships of the kingdom Animalia (Metazoa) have long been questioned. Whether the lowest eukaryotic multicellular organisms, the metazoan phylum Porifera (sponges), independently evolved multicellularity from a separate protist lineage (polyphyly of animals) or whether they were derived from the same protist group as the other animal phyla (monophyly) remains unclear. Analyses of the genes that are typical for multicellularity, e.g. those coding for adhesion molecules (galectin) and adhesion receptors (receptor tyrosine kinase, integrin receptor, receptors featuring scavenger receptor cysteine-rich domains) or elements involved in signal transduction pathways (G-protei…

Trans-sarcolemmal proteins situated central to the subsarcolemmal region

2002

Trans-sarcolemmal proteins located inside, within, and outside of the muscle fibre plasma membrane fall into two categories, the dystrophin-glycoprotein complex (DGC) and non-DGC-related proteins, e.g. dysferlin, caveolin, dystrobrevins and syntrophins. Mutational defects are responsible for their immunohistochemical absence or reduction giving rise to certain muscular dystrophies. In other neuromuscular disorders, i.e. inflammatory, metabolic, and neurogenic processes, transarcolemmal proteins are well preserved. Unlike desmin and plectin, which form a honeycomb-type network across the muscle fibre and a subsarcolemmal layer, trans-sarcolemmal proteins are not expressed central to the subs…

Towards an understanding of the molecular basis of immune responses in sponges: The marine demospongeGeodia cydonium as a model

1999

The phylogenetic position of the phylum Porifera (sponges) is near the base of the kingdom Metazoa. During the last few years, not only rRNA sequences but, more importantly, cDNA/genes that code for proteins have been isolated and characterized from sponges, in particular from the marine demosponge Geodia cydonium. The analysis of the deduced amino acid sequences of these proteins allowed a molecular biological approach to the question of the monophyly of the Metazoa. Molecules of the extracellular matrix/basal lamina, with the integrin receptor, fibronectin, and galectin as prominent examples, and of cell-surface receptors (tyrosine kinase receptor), elements of sensory systems (crystallin…