Search results for "Lectin"

Porifera Lectins: diversity, physiological roles and biotechnological potential

2015

An overview on the diversity of 39 lectins from the phylum Porifera is presented, including 38 lectins, which were identified from the class of demosponges, and one lectin from the class of hexactinellida. Their purification from crude extracts was mainly performed by using affinity chromatography and gel filtration techniques. Other protocols were also developed in order to collect and study sponge lectins, including screening of sponge genomes and expression in heterologous bacterial systems. The characterization of the lectins was performed by Edman degradation or mass spectrometry. Regarding their physiological roles, sponge lectins showed to be involved in morphogenesis and cell intera…

Multiple Sialyl Lewisx N-Glycopeptides: Effective Ligands for E-Selectin

1996

Multiple Sialyl-Lewisx-N-Glycopeptide: Effektive Liganden für E-Selectin

1996

Mechanisms of endothelial cell activation by endocannabinoid 2-arachidonoylglycerol

2020

Abstract Background Endothelial dysfunction promotes atherogenesis, vascular inflammation, and thrombus formation. Reendothelialization after angioplasty is required in order to restore vascular function and to prevent stent thrombosis. The endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) is a known modulator of inflammation. Earlier studies have demonstrated the relevance of this endocannabinoid in human pathophysiology during coronary artery disease and in murine experimental atherogenesis. However, evidence on the impact of 2-AG on endothelial cell function remains scarce. Methods Endothelial repair was studied in two treatment groups of wildtype mice following electrical denudation o…

Purification and molecular characterization of the rhamnose binding lectin from sea bass (Dicentrarchus labrax) that agglutinate Gram positive and ne…

2013

Evolution and Immune Function of Fish Lectins

2016

Abstract Lectins are sugar-binding proteins widely distributed among animals, plants, and microbial taxon, involved in diverse biological processes. In both invertebrates and vertebrates, they play key roles in nonself recognition and immune responses, such as nonself recognition, inflammatory processes, and immunomodulation. In fish, many lectin families have been identified, and their tissue-specific expression and localization of the various lectin repertoires and their ligands are consistent with their distinct biological roles in innate and adaptive immunity. Here, we discuss the involvement of F-type lectins, rhamnose-binding lectins, galectins, and C-type lectins in pathogen recognit…

Co-expression and Functional Interaction of Silicatein with Galectin

2006

Sponges (phylum Porifera) of the class of Demospongiae are stabilized by a siliceous skeleton. It is composed of silica needles (spicules), which provide the morphogenetic scaffold of these metazoans. In the center of the spicules there is an axial filament that consists predominantly of silicatein, an enzyme that catalyzes the synthesis of biosilica. By differential display of transcripts we identified additional proteins involved in silica formation. Two genes were isolated from the marine demosponge Suberites domuncula; one codes for a galectin and the other for a fibrillar collagen. The galectin forms aggregates to which silicatein molecules bind. The extent of the silicatein-mediated s…

Synthetic Glycopeptides from the E-Selectin Ligand 1 with Varied Sialyl Lewisx Structure as Cell-Adhesion Inhibitors of E-Selectin.

2007

Ibrutinib Abrogates TREM-1 Mediated Neutrophil Activation

2016

Abstract Triggering receptor expressed on myeloid cells 1 (TREM-1) is an activating receptor on neutrophils (PMN) and important in the innate host defence against microbial pathogens. Here we examined the influence of the Bruton tyrosine kinase (BTK) inhibitor ibrutinib on TREM-1 dependent activation of human PMNs. Firstly, ibrutinib specifically inhibited TREM-1 mediated PMN activation of the oxidative burst and CD62L shedding, whereas TLR mediated activation remained unaffected. Correspondingly, ibrutinib suppressed ERK phosphorylation after TREM-1, but not after TLR ligation. To clarify whether this TREM-1 specific effect of ibrutinib was also relevant in vivo, we treated mice with ibrut…

TRANSPLANTATION AND IN VITRO ANALYSIS OF SOYBEAN-AGGLUTININ SEPARATED MOUSE SPLEEN CELLS

1984

In human bone marrow allotransplantation, an in vitro method using soybean agglutinin(SBA) for enrichment of stem cells and depletion of mature T cells in the graft has been described (Reisner et al.;Lancet 2:1320,1980).To define the quality of the method,mouse spleen cells (C3H, C57/B6,BALB/c)containing about 30% of mature T cells were separated by SBA.The composition and functional capacity of the cell fraction known to contain the stem cells were characterized in vitro after each of two SBA-separation steps.In addition, the ability of the cell fraction to reconstitute successfully allogeneic irradiated (900R) mice was investigated. Only the two step SBA-separation procedure yielded satis…