Search results for "Lee"

Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life

2016

This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immu…

The addition of interleukin-2 to cyclophosphamide therapy can facilitate tumor growth of B16 melanoma.

1995

The role of interleukin-2 (IL-2) on tumor growth of B16F10 melanoma cells was assessed in two sets of mice with different immune status: normal (immunocompetent) mice and immunodeficient mice. The two sets of animals were treated with cyclophosphamide (CY) or IL-2 alone or with a combined therapy of CY+IL-2. On days 6 and 10 after tumor cell injection, we evaluated the incidence of hepatic B16 melanoma metastases and the percentage of hepatic volume occupied by metastatic tissue. We observed that the CY alone (300 mg/kg, days 3 and 8 post-tumoral inoculation) significantly reduced tumor growth in all treated mice; however, CY proved more effective in normal recipients than in immunodeficien…

Distribution and kinetics of superantigen-induced cytokine gene expression in mouse spleen.

1993

The polyclonal stimulation of T cells by bacterial superantigens is involved in the pathogenesis of the toxic shock syndrome in certain staphylococcal and streptococcal infections. Here we describe the onset and kinetics of superantigen-induced cytokine production in situ in spleens of normal BALB/c mice monitored at the level of cytokine mRNA expression by in situ hybridization. Messenger RNAs for interleukin 2 (IL-2), interferon gamma, and tumor necrosis factors (TNF) alpha and beta were not expressed at detectable levels in spleens of unstimulated animals but became visible already 30 min after intraperitoneal application of 50 micrograms staphylococcal enterotoxin B. All mRNA levels sho…

Effect of isoprinosine on IL-2, IFN-γ and IL-4 production in vivo and in vitro

1991

The effects of an immunopotentiating drug, isoprinosine, on the splenocytes of BALB/c mice to produce cytokines were investigated. Isoprinosine enhanced IL-2 production, upregulating the expression of IL-2 receptor in vitro. It also significantly increased the IFN-gamma secretion and decreased the IL-4 production in vivo. The significance of these findings in terms of immune regulation is discussed.

Is Aspirin a Worthy Candidate in Preventing Intracranial Aneurysm Rupture?

2020

Subarachnoid hemorrhage (SAH) usually occurs with a thunderclap headache or the worst headache of a patient's life, leading to a neurologic emergency. Among cases of nontraumatic SAH, 80%–85% are caused by ruptured intracranial aneurysms (IAs) and comprise 3% of all strokes in high-income countries In the years 2000–2008, the incidence of SAH was 4–7 per 100,000 person-years in high-income and low-to middle-income countries. Outcome after aneurysmal SAH depends on several factors, including severity of the initial hemorrhage, rebleeding, perioperative medical management, and timing and technical success for vascular malformation exclusion from the cerebral circulation. It has been estimated…

Detector for monitoring potential bleeding during electron intraoperative radiotherapy

2018

Purpose: The aim of this work is to develop a bleeding detector integrated into the acrylic circular applicators for specific mobile linacs. Thus, a bleeding detector has been developed based on a capacitive sensor to be used with plastic applicators, as in the case of LIAC HWL from Sordina IORT Technologies SpA. According to the clinical impact, we have selected 0.5 cm as the minimum depth of fluid that should be detected. Methods: An experiment was developed using water-simulating blood. Two setups were considered: non-beveled applicators with 7 cm and 10 cm diameter. Measurements were done for applicators 0 degrees and 45 degrees tilted, both with respect to the horizontal surface, in or…

Una gramática escolar interlingüística. Hacia una enseñanza reflexiva de las lenguas en contextos multilingües

2022

El reto de la educación plurilingüe requiere una enseñanza de las lenguas curriculares que promueva la reflexión sobre el uso y la transferencia lingüística para desarrollar la competencia comunicativa del alumnado. En este sentido, el proyecto Egramint se plantea la elaboración de una gramática escolar interlingüística como instrumento de formación docente y de intervención en el aula. Metodológica-mente, es una investigación de carácter cualitativo-etnográfico en tres fases. En la primera, sobre la que versa este artículo, abordamos el análisis documental de los currículos oficiales del ámbito español para delimitar cómo se configura en ellos la gramática escolar. Los resultados muestran …

Džeza loma Džeimsa Lī Burka "Deiva Robišo" detektīvromānu sērijā

2020

Džeimsa Lī Burka “Deiva Robišo” detektīvromāni ir plaši pazīstami literatūrcienītāju vidū. Papildus izciliem detektīvstāstiem, romāni piedāvā ieskatu 1980.-to gadu Ņūorleānā, kur tiek parādītas tādas tēmas kā vīrišķība, agresija, un rase. Šajā darbā tika aplūkota džeza tēma, un kā džeza terminoloģija palīdz uzturēt detektīvstāsta struktūru. Izmantojot izvēlēto darbu analīzi, interpretāciju, naratoloģiju un rūpīgu lasīšanu, darbā tika apskatīta džeza terminoloģija un tās sasaista ar detektīvstāstu. Darba rezultātā tika izstrādāta padziļināta džeza terminoloģijas analīze, un izskatīta tās ietekme uz detektīvromānu.

Suuresti suunniteltu : Keiteleen kanavan vaiheita

2017

Antibody synthesis in roach (Rutilus rutilus); analysis of antibody secreting cells in lymphoid organs with ELISPOT-assay

1994

The roach (Rutilus rutilus L.) which is a cyprinid fish, was immunised with bovine γ-globulin (BGG) and the antibody synthesis was studied by counting the number of specific antibody secreting cells (SASC) in the spleen and anterior kidney, and by measuring the antibody concentration in the circulation. SASCs and the total number of immunoglobulin secreting cells (TISC) were counted with the ELISPOT (enzyme-linked immunospot) assay, and anti-BGG antibodies and the concentration of immunoglobulin in sera were assayed by ELISA (enzyme-linked immunosorbent assay). The present modification of the ELISPOT-assay takes advantage of biotin-avidin amplification and yields easily detectable and nonfa…