Search results for "Lici"
showing 10 items of 2919 documents
The chaperone-like protein Cdc48 regulates ubiquitin-proteasome system in plants.
2021
The degradation of misfolded proteins is mainly mediated by the ubiquitin-proteasome system (UPS). UPS can be assisted by the protein Cdc48 but the relationship between UPS and Cdc48 in plants has been poorly investigated. Here, we analyzed the regulation of UPS by Cdc48 in tobacco thanks to two independent cell lines overexpressing Cdc48 constitutively and plant leaves overexpressing Cdc48 transiently. In the cell lines, the accumulation of ubiquitinated proteins was affected both quantitatively and qualitatively and the amount of proteasomal subunits was modified, while proteolytic activities were unchanged. Similarly, the over-expression of Cdc48 in planta impacted the accumulation of ub…
NMD-Based Gene Regulation—A Strategy for Fitness Enhancement in Plants?
2019
Abstract Post-transcriptional RNA quality control is a vital issue for all eukaryotes to secure accurate gene expression, both on a qualitative and quantitative level. Among the different mechanisms, nonsense-mediated mRNA decay (NMD) is an essential surveillance system that triggers degradation of both aberrant and physiological transcripts. By targeting a substantial fraction of all transcripts for degradation, including many alternative splicing variants, NMD has a major impact on shaping transcriptomes. Recent progress on the transcriptome-wide profiling and physiological analyses of NMD-deficient plant mutants revealed crucial roles for NMD in gene regulation and environmental response…
Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco
2017
SPEIPMUBINRASUPDATDOCT; Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both th…
Alternative Splicing of the Basic Helix–Loop–Helix Transcription Factor Gene CmbHLH2 Affects Anthocyanin Biosynthesis in Ray Florets of Chrysanthemum…
2021
Chrysanthemum is an important ornamental crop worldwide. Some white-flowered chrysanthemum cultivars produce red ray florets under natural cultivation conditions, but little is known about how this occurs. We compared the expression of anthocyanin biosynthetic and transcription factor genes between white ray florets and those that turned red based on cultivation conditions to comprehend the underlying mechanism. Significant differences in the expression of CmbHLH2 were detected between the florets of different colors. CmbHLH2 generated two alternatively spliced transcripts, designated CmbHLH2Full and CmbHLH2Short. Compared with CmbHLH2Full, CmbHLH2Short encoded a truncated protein with only…
Hemocyanin genes as indicators of habitat shifts in Panpulmonata?
2018
Hemocyanin is the primary respiratory protein for the majority of the Mollusca and therefore directly interfaces with the physiological requirements of each species and the environments to which they are adapted. Hemocyanin is therefore likely to have been evolutionarily imprinted by significant habitat shifts. In the gastropod clade Panpulmonata (>30,000 species) major realm transitions have occurred multiple times independently and may have contributed to the diversification of this group. Yet, little is known about the adaptive changes linked to these habitat shifts. In order to gain deeper insight into the evolution of panpulmonate hemocyanins and to infer possible impacts associated wi…
Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell
2003
Five cDNAs encoding Rac protein homologues to the Rho-related proteins from plants (Rop) were isolated in tobacco, and the function of one of them, Ntrac5, was studied. The Ntrac5 mRNA is repressed when tobacco leaves and cells are treated with the fungal elicitor cryptogein. Tobacco cells were transformed with sense constructs of Ntrac5 or Ntrac5V15, encoding the native GTP/GDP-bound form of this Rac protein homologue or the constitutively active mutant in its GTP-bound form, respectively. Immunological studies indicate that the corresponding protein is continuously located on the plasma membrane (PM). Both types of transformed cells show the same extra-cellular alkalinization as the contr…
Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilization of internal calcium
2003
Abstract Ergosterol interacts with tobacco suspension ( Nicotiana tabacum ) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Compared with the responses induced by cryptogein, a proteinaceous elicitor from Phytophthora sp., oxidative burst and ΔpH changes were weaker whereas phytoalexin accumulation was higher with ergosterol. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of…
A phytosociological analysis of the Olea europaea L. var. sylvestris (Mill.) Lehr. forests in Sicily
2019
This paper presents a phytosociological study on the forest vegetation of Olea europaea var. sylvestris of Sicily and of the smaller minor islands. In Sicily, Oleaster formations show considerable climacic potentiality in the bioclimactic belts between the infra- and the thermomediterranean with single edapho-climacic penetrations that are also in the mesomediterranean; however, these were largely destroyed by man in order to make room for crops. Furthermore, the residual expressions of the Oleaster forests are limited, and often exist as regenerated woodland made possible by the abandonment of agricultural land; they are often small forest nuclei–high maquis, woods and micro-woods–with a m…
Intracellular compartmentation and plant cell signalling
1997
Compartmentation is an essential feature of eukaryotic cells, and is crucial for the regulation of cell metabolism. Recent progress has significantly improved the understanding of signal transduction pathways in plants, including the activation of light-signalling networks and the tightly controlled generation of the calcium message. Cell compartmentation is important for the regulation and proliferation of these signalling processes.
Evaluation of fruit quality and antioxidant activity of kiwifruit during ripening and after storage
2016
BACKGROUND: In the Northern hemisphere kiwifruit harvest time is conditioned by low temperatures occurring during the harvesting season while in Southern Italy minimum temperatures in autumn are high enough to support fruit growth and ripening. OBJECTIVE: To evaluate the influence of delayed harvest time on kiwifruit quality attributes. METHODS: The experiment was carried out in a commercial kiwifruit, 'Hayward', orchard located in Southern Italy. In this area, the conventional harvest time occurs 160 days after full bloom (DAFB), during the first decade of November. Fruits were collected from 130 DAFB to 192 DAFB. Fresh fruit and dry weight, flesh color, firmness, total soluble solids cont…