Search results for "Linum"

Inhibition of FcεRI-mediated Activation of Rat Basophilic Leukemia Cells by Clostridium difficile Toxin B (Monoglucosyltransferase)

1996

Abstract Treatment of rat basophilic leukemia (RBL) 2H3-hm1 cells with Clostridium difficile toxin B (2 ng/ml), which reportedly depolymerizes the actin cytoskeleton, blocked [3H]serotonin release induced by 2,4-dinitrophenyl-bovine serum albumin, carbachol, mastoparan, and reduced ionophore A23187-stimulated degranulation by about 55-60%. In lysates of RBL cells, toxin B 14C-glucosylated two major and one minor protein. By using two-dimensional gel electrophoresis and immunoblotting, RhoA and Cdc42 were identified as protein substrates of toxin B. In contrast to toxin B, Clostridium botulinum transferase C3 that selectively inactivates RhoA by ADP-ribosylation did not inhibit degranulation…

Principi di spontaneizzazione in Sicilia di Talinum paniculatum (Talinaceae)

2017

Record of Talinum paniculatum (Talinaceae) naturalized in Sicily. – The record in the urban context of Palermo (Sicily) of Talinum paniculatum, species native to Tropical America and mainly cultivated for ornamental purposes, is reported. It is the first case of naturalization in the Island of a species of Talinaceae family introduced to Palermo in 1984 from Argentina and cultivated in the Botanical Garden collections.

Botulinum Toxin Is Effective in the Management of Neurogenic Dysphagia. Clinical-Electrophysiological Findings and Tips on Safety in Different Neurol…

2017

Background and Aims: Neurogenic dysphagia linked to failed relaxation of the upper esophageal sphincter (UES) can be treated by injecting botulinum toxin (BTX) into the cricopharyngeal (CP) muscle. We compared the effects of this treatment in different neurological disorders with dysphagia, to evaluate its efficacy over time including the response to a second injection. Materials and Methods: Sixty-seven patients with neurogenic dysphagia associated with incomplete or absent opening of the UES (24 with brainstem or hemispheric stroke, 21 with parkinsonian syndromes, 12 with multiple sclerosis, and 10 with spastic-dystonic syndromes secondary to post-traumatic encephalopathy) were treated wi…

Cytotoxic activity of diterpenoids isolated from the aerial parts of Elaeoselinum asclepium subsp. meoides.

2008

The phytochemical investigation of the acetone extract of the aerial parts of Elaeoselinum asclepium (L.) Bertol. subsp. meoides (Desf.) Fiori afforded several known diterpenoids as well as meoidic acid ( 5), new in the literature. The cytotoxic activities of elasclepic acid ( 1), ENT-atis-16-en-19-oic acid ( 2), ent-beyer-15-en-19-oic acid ( 3), ent-kaur-16-en-19-oic acid ( 4) and meoidic acid ( 5) were investigated on rat glioma C6 cells by evaluation of cell growth inhibition.

Botulinum toxin injections into the detrusor

2011

Modeling the effect of ethanol vapor on the germination time of Penicillium chrysogenum.

2005

The influence of ethanol vapor on germination of Penicillium chrysogenum was determined on yeast nitrogen base plus glucose agar medium at 25 degrees C. Ethanol vapors were generated by 0 to 6% (wt/wt) ethanol solutions at the bottom of hermetically closed petri dishes. The logistic equation was used to describe the data as the percentage of germination versus time and to estimate the germination time. The effect of ethanol concentration on germination time was described by a new reparameterized equation, resulting in an estimated limiting ethanol concentration of 4.3%. Up to 3% ethanol, all spores germinated, and the germination time increased with increasing ethanol concentration. At 3.5 …

Recent progress in optical and electrochemical biosensors for sensing of Clostridium botulinum neurotoxin

2018

Abstract Botulinum toxin is a neurotoxic protein which produced from Clostridium botulinum and related species and it block acetylcholine release from presynaptic nerve terminals at the neuromuscular junctions. This toxin is life threatening for millions of people and growing menace to society since causing human botulism. Enzymatic activity of Botulinum neurotoxin within the cell made it hazardous and lead to flaccid paralysis. However, there isn't any reliable and precise remedy for this toxin. Therefore, there is an urgent need for early detection of this toxin in a fast and meticulous way with a robust and cost-effective relationship for real-time monitoring of Botulinum neurotoxin. Sev…

Oromandibular dystonia: A dental approach

2010

Oromandibular dystonia consists of prolonged spasms of contraction of the muscles of the mouth and jaw. Primary idiopathic forms and secondary forms exist. Secondary dystonia develops due to environmental factors; some cases of cranial dystonia after dental procedure have been reported, but the causal relationship between these procedures and dystonia remains unclear. Traumatic situations in the mouth, such as poor aligned dentures or multiple teeth extractions may cause an impairment of proprioception of the oral cavity, leading to subsequent development of dystonia. The clinical characteristics of oromandibular dystonia are classified according to the affected muscles. The muscles involve…

Sensitivity to acetic acid, ability to colonize abiotic surfaces and virulence potential of Listeria monocytogenes EGD-e after incubation on parsley …

2010

International audience; Abstract Aim: To investigate how the survival of Listeria monocytogenes on parsley leaves may affect its ability to sustain process-related harsh conditions and its virulence. Methods and Results: Parsley seedlings were spot inoculated with stationary phase cells of L. monocytogenes EGD-e and incubated for 15 days. Each day, bacterial cells were harvested and enumerated, and their ability to survive acetic acid challenge (90 min, pH 4.0), to colonize abiotic surfaces and to grow as biofilms was assessed. After a 3-log decrease over the first 48 h, the population stabilized to about 10(6) CFU g(-1) until the sixth day. After the sixth day, L. monocytogenes was no long…

Amber Particles as Living Plant Cell Markers in Flow Cytometry / Dzintara Daļiņas Kā Dzīvu Augu Šūnu Marķieri Plūsmas Citometrijā

2015

Abstract The unique biological properties of amber are well known. Amber particles that penetrate into the cellular matrix can potentially be used as markers of plant cell biological activity by identification of living cells in flow cytometry. However, there have been no studies on effect of amber particles on plant cells. The aim of this study was to determine effect of amber nano- and micro- (5 nm-3 μm) particles on somatic and gametic cells and to assess the possibility to use amber particles as alive plant cells markers. To reach the aim, fluorescence of cells was determined in the presence of amber particles, and amber components - spirit of amber and sodium succinate dibasic hexahydr…