Search results for "MAI"
showing 10 items of 6279 documents
Protein quality control during aging involves recruitment of the macroautophagy pathway by BAG3.
2009
The Hsc/Hsp70 co-chaperones of the BAG (Bcl-2-associated athanogene) protein family are modulators of protein quality control. We examined the specific roles of BAG1 and BAG3 in protein degradation during the aging process. We show that BAG1 and BAG3 regulate proteasomal and macroautophagic pathways, respectively, for the degradation of polyubiquitinated proteins. Moreover, using models of cellular aging, we find that a switch from BAG1 to BAG3 determines that aged cells use more intensively the macroautophagic system for turnover of polyubiquitinated proteins. This increased macroautophagic flux is regulated by BAG3 in concert with the ubiquitin-binding protein p62/SQSTM1. The BAG3/BAG1 ra…
R-Baklofēna stress testējumi un to piemaisījumu noteikšanas metodes validācija
2018
R-Baklofēna stress testējumi un to piemaisījumu noteikšanas metodes validācija. Goliškina S.M., zinātniskie vadītāji: asoc. prof., Dr. ķīm. P. Mekšs, Dr.ķīm. A. Bolotin. Maģistra darbs, 61 lappuses, 25 attēli, 26 tabulas, 31 literatūras avoti. Latviešu valodā. Darbā ir apkopota literatūra par dažādām AEŠH metodēm, lai noteiktu baklofēnu un tā enantiomērus bioloģiskajos šķidrumos un gatavās zāļu formās, kā arī piemaisījumu noteikšanas metodi. Literatūras apskatā apkopotā informācija ietver laika periodu no 1985. līdz 2017. gadam. Eksperimentālajā daļā tika veikta baklofēna stresa testēšana, un rezultātus novērtēja, izmantojot izstrādātu AEŠH metodi baklofēna piemaisījumu noteikšanai. AEŠH-MS…
The Effect of CMC in Business Emails in Lingua Franca: Discourse Features and Misunderstandings
2018
The paper argues that everyday exchange of business emails produces a development in the work-group relationship, which, in turn, makes new communication styles possible and acceptable by the users' habit to computer-mediated forms, even in unbalanced professional exchanges. The focus is on the (spoken) discourse features of email messages in a self-compiled corpus of selected computer-mediated business emails, produced by five participants over three months (October 2015 – February 2016). The exchange, involving the use of English by non-native speaker interactants (in particular, Business English as a Lingua Franca (BELF)), as well as language adjustments in a computer-mediated exchange, …
Interactions surface continentale/atmosphère : l'expérience HAPEX-Sahel
1994
L'évolution saisonnière des termes du bilan radiatif, du bilan d'énergie et la production primaire d'une savane humide d'Afrique de l'Ouest (Lamto, Côte d'Ivoire) a été suivie dans le cadre du programme SAvanes à Long Terme (SALT). Ces données ont permis de développer un modèle de simulation des échanges surface/atmosphère en savane humide, basé sur les relations fonctionnelles existant entre la production et la phénologie de la savane d'une part, et les bilans d'énergie et hydrique de ce type de surface d'autre part. Ce modèle est issu du couplage de deux sous-modèles fonctionnant au pas de temps journalier et utilisant des variables climatiques standard en entrée : le modèle de bilan d'én…
Preparation and Characterization of P2 BCh Ring Systems (Ch=S, Se) and Their Reactivity with N-Heterocyclic Carbenes
2018
Four-membered rings with a P2BCh core (Ch = S, Se) have been synthesized via reaction of phosphinidene chalcogenide (Ar*P=Ch) and phosphaborene (Mes*P=BNR2). The mechanistic pathways towards these rings are explained by detailed computational work that confirmed the preference for the formation of P–P, not P–B, bonded systems, which seems counterintuitive given that both phosphorus atoms contain bulky ligands. The reactivity of the newly synthesized heterocycles, as well as that of the known (RPCh)n rings (n = 2, 3), was probed by the addition of Nheterocyclic carbenes, which revealed that all investigated compounds can act as sources of low-coordinate phosphorus species. peerReviewed
Communication strategies used by 'British Petroleum (BP)' to deal with legitimacy before and after the Deepwater Horizon Accident
2016
Oil spills pose serious threats to the legitimacy of the companies involved and the oil industry in general. The objective of this study is to conduct research on the communication strategies used by ‘British Petroleum’ (BP) to maintain its legitimacy before the Deepwater Horizon (DH) oil spill caused in April 2010 and to repair its legitimacy after the DH accident. There is existing literature on the legitimacy theory and the communication strategies as well as DH accident and its causes. A qualitative study based on the sustainability reports of BP from 2006 to 2015 and its press releases in relation to the DH accident from April 2010 to April 2016 is conducted to discover its preferred c…
Quand le sport (dés)oriente les parcours des jeunes
2020
Référence interne : 20013; Axé sur la transition lycée-enseignement supérieur, ce rapport souligne la diversité des parcours vécus par des bacheliers et notamment des bacheliers professionnels dans le secondaire, en montrant leurs rapports pluriels aux études. Il étudie tout particulièrement comment des expériences et investissements sportifs peuvent impacter les orientations des jeunes, voire leur donner un tour inattendu, à l'image des bacheliers professionnels en STAPS ou des jeunes filles en filière masculine. L’expérience en lycée professionnel peut être perçue par certains comme une possibilité d'apprendre un métier ou d’obtenir le baccalauréat, d'autres la vivent comme une fermeture …
Production and characterization of Bacillus thuringiensis Cry1Ac-resistant cotton bollworm Helicoverpa zea (Boddie).
2007
ABSTRACT Laboratory-selected Bacillus thuringiensis -resistant colonies are important tools for elucidating B. thuringiensis resistance mechanisms. However, cotton bollworm, Helicoverpa zea , a target pest of transgenic corn and cotton expressing B. thuringiensis Cry1Ac (Bt corn and cotton), has proven difficult to select for stable resistance. Two populations of H. zea (AR and MR), resistant to the B. thuringiensis protein found in all commercial Bt cotton varieties (Cry1Ac), were established by selection with Cry1Ac activated toxin (AR) or MVP II (MR). Cry1Ac toxin reflects the form ingested by H. zea when feeding on Bt cotton, whereas MVP II is a Cry1Ac formulation used for resistance se…
Genetic variability of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) populations from Latin America is associated with variations in susceptib…
2006
ABSTRACT Bacillus thuringiensis strains isolated from Latin American soil samples that showed toxicity against three Spodoptera frugiperda populations from different geographical areas (Mexico, Colombia, and Brazil) were characterized on the basis of their insecticidal activity, crystal morphology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of parasporal crystals, plasmid profiles, and cry gene content. We found that the different S. frugiperda populations display different susceptibilities to the selected B. thuringiensis strains and also to pure preparations of Cry1B, Cry1C, and Cry1D toxins. Binding assays performed with pure toxin demonstrated that the differences in the …
Clostridium difficile Toxins Disrupt Epithelial Barrier Function by Altering Membrane Microdomain Localization of Tight Junction Proteins
2001
ABSTRACT The anaerobic bacterium Clostridium difficile is the etiologic agent of pseudomembranous colitis. C. difficile toxins TcdA and TcdB are UDP-glucosyltransferases that monoglucosylate and thereby inactivate the Rho family of GTPases (W. P. Ciesla, Jr., and D. A. Bobak, J. Biol. Chem. 273:16021–16026, 1998). We utilized purified reference toxins of C. difficile , TcdA-10463 (TcdA) and TcdB-10463 (TcdB), and a model intestinal epithelial cell line to characterize their influence on tight-junction (TJ) organization and hence to analyze the mechanisms by which they contribute to the enhanced paracellular permeability and disease pathophysiology of pseudomembranous colitis. The increase i…