Search results for "MCR-1"

showing 4 items of 4 documents

Detection of plasmid-mediated colistin resistance, mcr-1 gene, in Escherichia coli isolated from high-risk patients with acute leukemia in Spain

2019

Background: Bacterial infections in immunocompromised patients are associated with a high mortality and morbidity rate. In this high-risk group, the presence of multidrug-resistant (MDR) bacteria, particularly bacteria that harbor a transferable antibiotic resistance gene, complicates the management of bacterial infections. In this study, we investigated the presence of the transferable colistin resistance mcr genes in patients with leukemia in Spain. Methods: 217 fecal samples collected in 2013-2015 from 56 patients with acute leukemia and colonized with MDR Enterobacteriaceae strains, were screened on September 2017 for the presence of the colistin resistance mcr genes (mcr-1 to -5) by mu…

0301 basic medicineMicrobiology (medical)medicine.drug_class030106 microbiologyAntibioticsColistin resistanceMicrobial Sensitivity TestsBiologymedicine.disease_causebeta-LactamasesMicrobiologyLeukemic patients03 medical and health sciencesMinimum inhibitory concentration0302 clinical medicinePlasmid[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesDrug Resistance BacterialmedicineEscherichia coliHumansPharmacology (medical)[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology030212 general & internal medicineEscherichia coliEscherichia coli Infections[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseasesLeukemiaColistinEscherichia coli ProteinsMiddle Agedbiology.organism_classificationEnterobacteriaceae[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology3. Good healthAnti-Bacterial AgentsInfectious DiseasesSpainColistin resistance Escherichia coli Leukemic patients Spain mcr-1 gene[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyColistinMultilocus sequence typingmcr-1 geneMCR-1hormones hormone substitutes and hormone antagonistsmedicine.drugPlasmids
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mcr-1- like detection in commensal Escherichia coli and Salmonella spp. from food-producing animals at slaughter in Europe

2017

International audience; We evaluate here the presence of the mcr-1-like and mcr-2 genes in Escherichia coli and Salmonella spp. isolated from healthy food-producing animals at slaughter between 2002 and 2014 in Europe. Isolates were retrieved from cattle, pig and chicken from 11 European countries of production. The susceptibility to colistin and antibiotics used in human medicine was determined by agar dilution. Colistin-resistant isolates were PCR-screened for mcr genes. mcr-positive isolates were typed by Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing. Among the 10,206 E. coli and 1774 Salmonella spp. isolated from cattle, pigs and chickens, 148 E. coli and 92 Sa…

0301 basic medicineSalmonellaVeterinary medicineFood-producing animalsmedicine.drug_classSwine030106 microbiologyAntibioticsBiology[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriologymedicine.disease_causeMicrobiologyAgar dilution03 medical and health sciencesBacterial Proteins[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologySalmonellaDrug Resistance BacterialmedicinePulsed-field gel electrophoresisEscherichia coliAnimalsTypingEscherichia coliEscherichia coli Infections2. Zero hungerSalmonella Infections AnimalGeneral VeterinaryColistinEscherichia coli ProteinsGeneral Medicine[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology3. Good healthAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldEuropeSalmonella spp.ColistinMCR-1CattleChickensMCR-1Abattoirshormones hormone substitutes and hormone antagonistsmedicine.drugMultilocus Sequence Typing
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Kolistīna rezistento Escherichia coli izolātu ģenētiskais raksturojums

2018

Antibiotiku rezistence ir aktuāla problēma, kas izraisījusi satraukumu par iespējām ārstēt bakteriālās infekcijas. Kolistīns ir plaša spektra antibiotika, kas tiek lietota pret multirezistentiem baktēriju celmiem. Rezistenci pret kolistīnu nodrošina mcr gēni, kas var būt kodēti plazmīdās un hromosomās. Pētījumā noteikta kolistīna rezistence pārtikas un cūku aklo zarnu paraugos. Paraugi iegūti Valsts Pētījumu Programmas “Lauksaimniecības resursi ilgtspējīgai kvalitatīvas un veselīgas pārtikas ražošanai Latvijā (AgroBioRes)” projekta Nr. 5 “Mikroorganismu rezistences un citu bioloģisko un ķīmisko risku izpētes procedūru izstrāde un pielietošanas pārtikas ķēdē (RISKI)” ietvaros no 2015. līdz 2…

EscherichiacolikolistīnaBioloģijarezistencemcr-1
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Kolistīna rezistento Escherichia coli baktēriju rezistences raksturojums, izmantojot mikrobioloģiskās un molekulārās bioloģijas metodes

2016

Darba mērķis bija izpētīt Pārtikas drošības, dzīvnieku veselības un vides zinātniskajā institūtā "BIOR" izolētas multirezistentas Escherichia coli kultūras, nosakot gēna mcr-1 un plazmīdu klātbūtni pret kolistīnu rezistento kultūru paraugos. Bakalaura darbs izstrādāts no 2016. gada februāra līdz 2016. gada maijam Pārtikas drošības, dzīvnieku veselības un vides zinātniskajā institūtā “BIOR” Dzīvnieku slimību diagnostikas laboratorijā, mikrobioloģijas un virusoloģijas nodaļās un Latvijas Biomedicīnas pētījumu un studiju centrā (BMC), Rekombinantu biotehnoloģijas grupā. Pētāmie paraugi tika iegūti no lauksaimniecības dzīvniekiem un pārtikas produktiem 2014. un 2015. gada VPP (Valsts pētījumu p…

transformācijakolistīnaBioloģijarezistencemcr-1gēns
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