Search results for "MELTING CURVE"

showing 4 items of 14 documents

Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chai…

2009

9 pages.

Ochratoxin AAspergillus niger aggregateGrapesHealth Toxicology and MutagenesisFood ContaminationWineAspergillus carbonariusBiologyToxicologyPolymerase Chain ReactionSensitivity and SpecificityMelting curve analysisReal-time polymerase chain reactionlaw.inventionMicrobiology03 medical and health scienceschemistry.chemical_compoundSpecies SpecificitylawTaqManVitisDNA FungalOchratoxinPolymerase chain reaction030304 developmental biology2. Zero hunger0303 health sciencesMycotoxinChromatography030306 microbiologyPublic Health Environmental and Occupational HealthFungal geneticsOchratoxin AGeneral ChemistryGeneral MedicineMycotoxinsSpores FungalOchratoxins3. Good healthAspergillusReal-time polymerase chain reactionchemistrySpainCarcinogensSYBR Green IAspergillus tubingensisPolyketide synthaseFood Science
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Single tube optimisation of APOE genotyping based on melting curve analysis

2008

Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Results: Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. Conclusions: This method is cheaper, faster and more reliable and versatile than other alternatives proposed. © 2008 Elsevier Inc. All rights reserved.

Protocol (science)GenotypeComputer scienceClinical BiochemistryNanotechnologyGeneral MedicinePolymerase Chain ReactionMelting curve analysisSingle tubeApolipoproteins EValidation studiesCardiovascular DiseasesHumansThermodynamicsGenotypingAlgorithmAnalysisDNA PrimersClinical Biochemistry
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Exploring evolutionary responses to increasing temperature in an environmental opportunistic pathogen

2017

bakteeritauditluonnonvalintafylogeniahigh resolution melting curvelämmönsietoympäristötekijätvirulenssievoluutioselectionkalatauditgenotyyppibakteeritvirulencethermal performance curveFlavobacterium columnaretaudinaiheuttajatsekvenssianalyysilämpötilamulti-locus sequence analysis
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Kristallisation und schmelzverhalten von fraktionen verzweigten polyethylens, 1. Darstellung und charakterisierung der fraktionen

1983

A branched polyethylene with broad molecular weight distribution was separated into a series of fractions by fractional precipitation from dilute solution. The fractions were characterized by viscometric and density measurements, IR-spectroscopy and calorimetry. The methyl group content and the density turned out as largely constant. Molecular weight effects show up in the melting behaviour. The main range of melting and the end of the melting curve shift to lower temperatures with decreasing molecular weight. Compared to the overall extension of the melting range these shifts are small. One has to conclude that polydispersity gives only a minor contribution to the broadening of the melting…

chemistry.chemical_compoundChemistryDispersityPolymer chemistryPartial meltingMolar mass distributionFractionationCalorimetryPolyethyleneMelting curve analysisMethyl groupDie Makromolekulare Chemie
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