6533b7d8fe1ef96bd1269a18

RESEARCH PRODUCT

Single tube optimisation of APOE genotyping based on melting curve analysis

Dolores CorellaDolores CorellaO. PortolesMarisa GuillénJosé V. SorlíJosé V. SorlíFrancesc FrancésJosé I. GonzálezJosé I. González

subject

Protocol (science)GenotypeComputer scienceClinical BiochemistryNanotechnologyGeneral MedicinePolymerase Chain ReactionMelting curve analysisSingle tubeApolipoproteins EValidation studiesCardiovascular DiseasesHumansThermodynamicsGenotypingAlgorithmAnalysisDNA Primers

description

Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Results: Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. Conclusions: This method is cheaper, faster and more reliable and versatile than other alternatives proposed. © 2008 Elsevier Inc. All rights reserved.

yearjournalcountryeditionlanguage
2008-07-01Clinical Biochemistry
https://doi.org/10.1016/j.clinbiochem.2008.03.010