Search results for "MHC Class II"

showing 10 items of 82 documents

The HLA locus and multiple sclerosis in Sicily

2005

The authors report the analysis of HLA-class II allelic heterogeneity in a well characterized multiple sclerosis (MS) Sicilian dataset. Family-based association analysis revealed evidence for excess transmission to affected individuals for alleles HLA-DRB1*1501, DRB1*04, and DQB1*0302. When analyzed as haplotypes, the authors observed excess transmission for the DRB1*0400-DQB1*0302 haplotype. Sicilian patients share the HLA-DRB1*1501 susceptibility allele with affecteds living in continental Italy, but also display the allelic heterogeneity that characterizes Mediterranean populations.

AdultMaleRiskmusculoskeletal diseasesMultiple SclerosisAdolescentGenes MHC Class IILocus (genetics)Human leukocyte antigenBiologySeverity of Illness IndexLinkage DisequilibriumCohort StudiesDisability EvaluationGene Frequencyimmune system diseasesMultiple Sclerosis/epidemiologyPrevalencemedicineHumansGenetic Predisposition to DiseaseAge of OnsetAlleleskin and connective tissue diseasesSicilyAllelesGenetic associationGeneticsHLA-D AntigensIncidenceMultiple sclerosisHaplotypeGene Poolmedicine.diseaselanguage.human_languageSettore BIO/18 - GeneticaHaplotypeslanguageFemaleAllelic heterogeneitySettore MED/26 - NeurologiaNeurology (clinical)Sicilian
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Relationship Between Human Leucocyte Antigen Class I and Class II and Chronic Idiopathic Urticaria Associated With Aspirin and/or NSAIDs Hypersensiti…

2006

Background. HLA genes play a role in the predisposition of several diseases. The aim was to analyze the prevalence of HLA class I phenotypes and HLA-DRB1*genotype in patients with CIU associated with ASA and NSAIDs hypersensitivity (AICU).Methods. 69 patients with AICU, and 200 healthy subjects.Results. Subjects with HLA-B44 and HLA-Cw5 antigens were more represented in patients with AICU than in control group. Subjects with HLA-A11, HLA-B13, HLACw4, and HLA-Cw7 antigen were more represented in control group than in patients with AICU. Multiple logistic regression demonstrated an association of HLA-Cw4 and HLA-Cw7 with a lower risk of AICU, whereas carriers of HLA-B44 phenotype had a higher…

AdultMaleSettore MED/09 - Medicina InternaChronic Idiopathic UrticariaGenotypeUrticariahuman leucocyte antigen class IImmunologyGenes MHC Class IIAnti-Inflammatory AgentsHuman leukocyte antigenLower riskDrug HypersensitivityResearch CommunicationAntigenGene FrequencyRisk FactorsGenotypelcsh:PathologyMedicineHumansAlleleAllele frequencyAllelesAspirinAspirinbusiness.industryAnti-Inflammatory Agents Non-SteroidalHistocompatibility Antigens Class ICase-control studyCell BiologyHLA-DR AntigensMiddle AgedNSAIDhuman leucocyte antigen class I; human leucocyte antigen class II; chronic idiopathic urticaria; aspirin; NSAIDs; hypersensitivityhuman leucocyte antigen class IIMHC Class IIPhenotypeGenesCase-Control StudiesImmunologyFemalehypersensitivityNon-Steroidalbusinesslcsh:RB1-214medicine.drugHLA-DRB1 ChainsMediators of Inflammation
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Major histocompatibility complex and sporadic Alzheimer's disease: a critical reappraisal

2003

Epidemiological data suggest that some genetic determinants of Alzheimer's disease (AD) might reside in those polymorphisms for the immune system genes that regulate immune inflammatory responses, such as the major histocompatibility complex (MHC). Therefore, MHC polymorphisms have been the focus of a large number of AD association studies. Class Ia, Ib (hemochromatosis gene (HFE)), class II and class III (complement, tumour necrosis factor and heat shock proteins) alleles have been studied. Nearly every positive result has been followed by several studies that have failed to replicate it or that have contradicted it. Several factors, including methodological biases, might explain these dis…

AgingGenes MHC Class IIGenes MHC Class ILocus (genetics)Human leukocyte antigenMajor histocompatibility complexBiochemistryMajor Histocompatibility ComplexEndocrinologyAlzheimer DiseaseMHC class IGeneticsHumansGenetic Predisposition to DiseaseAlleleMolecular BiologyAllelesAgedGenetic associationGeneticsbiologyTumor Necrosis Factor-alphaHaplotypeCell BiologyHistocompatibilitybiology.proteinExperimental Gerontology
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MHC class II-expressing hepatocytes function as antigen-presenting cells and activate specific CD4 T lymphocyutes.

2003

The ability to activate CD4 T cells is restricted to antigen-presenting cells that express major histocompatibility complex (MHC) class II molecules. Parenchymal cells normally do not express MHC class II molecules; however, in clinical hepatitis, viral or autoimmune, hepatocytes often exhibit aberrant MHC class II expression. It is not known whether MHC class II-expressing hepatocytes can function as antigen-presenting cells, but it has been suggested that aberrant MHC class II expression by parenchymal cells may cause autoimmune disease. Therefore, we generated transgenic mice that specifically overexpress class II transactivator molecules in hepatocytes. Hepatocytes from these mice exhib…

CD4-Positive T-LymphocytesCD74Antigen presentationCD1Antigen-Presenting CellsGene ExpressionMice Inbred StrainsMice TransgenicLymphocyte ActivationHepatitisMiceMHC class ICytotoxic T cellAnimalsMHC class IIHepatologybiologyAntigen processingHistocompatibility Antigens Class IINuclear ProteinsMHC restrictionCell biologyImmunologybiology.proteinHepatocytesTrans-ActivatorsHepatology (Baltimore, Md.)
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MHC class II tetramer guided detection of Mycobacterium tuberculosis-specific CD4+ T cells in peripheral blood from patients with pulmonary tuberculo…

2007

Novel diagnostic tools are needed to diagnose latent infection and to provide biologically meaningful surrogate markers to define cellular immune responses against Mycobacterium tuberculosis (MTB). Interferon gamma-based assays have recently been developed in addition to the more than 100-year-old tuberculin skin test (TST) for the immune diagnosis of MTB in blood. The advent of soluble MHC/peptide tetramer molecules allows to objectively enumerate antigen-specific T cells. We identified novel MHC class II-restricted MTB epitopes and used HLA-DR4 tetrameric complexes to visualize ex vivo CD4(+) T cells directed against the antigens Ag85B and the 19-kDa lipoprotein, shared between MTB and ot…

CD4-Positive T-LymphocytesImmunologyMolecular Sequence DataEpitopes T-Lymphocytechemical and pharmacologic phenomenaMajor histocompatibility complexEpitopeImmune systemAntigenMHC class IHumansAmino Acid SequenceTuberculosis PulmonaryMHC class IIAntigen PresentationAntigens BacterialbiologyHistocompatibility Antigens Class IICD28General MedicineMycobacterium tuberculosisrespiratory systembacterial infections and mycosesVirologyImmunologybiology.proteinCD8Scandinavian journal of immunology
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Defective T helper response of hepatocyte-stimulated CD4 T cells impairs antiviral CD8 response and viral clearance.

2007

Background & Aims: In hepatitis, hepatocytes gain the ability to express major histocompatibility complex (MHC) class II molecules and to present antigen to CD4 T cells. Here, we investigated whether MHC class II-expressing hepatocytes influence in vitro the differentiation of CD4 T cells and in vivo the T-cell response to and control of viral infection. Methods: Class II transactivator-transgenic hepatocytes that constitutively express MHC class II molecules were used to stimulate CD4 T cells in vitro, and the effector response type of the stimulated CD4 T cells was determined. The in vivo relevance of the obtained findings was confirmed by infecting nontransgenic or class II transactivato…

CD4-Positive T-LymphocytesMHC class IIHepatologybiologyCD8 AntigensGastroenterologyCD1Mice TransgenicT helper cellT-Lymphocytes Helper-InducerMHC restrictionCD8-Positive T-LymphocytesMicemedicine.anatomical_structureMHC class IImmunologyCD4 Antigensbiology.proteinmedicineHepatocytesCytotoxic T cellAnimalsAntigen-presenting cellCD8Gastroenterology
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Increased antigen presentation efficiency by coupling antigens to MHC class I trafficking signals.

2007

Abstract Genetic modification of vaccines by linking the Ag to lysosomal or endosomal targeting signals has been used to route Ags into MHC class II processing compartments for improvement of CD4+ T cell responses. We report in this study that combining an N-terminal leader peptide with an MHC class I trafficking signal (MITD) attached to the C terminus of the Ag strongly improves the presentation of MHC class I and class II epitopes in human and murine dendritic cells (DCs). Such chimeric fusion proteins display a maturation state-dependent subcellular distribution pattern in immature and mature DCs, mimicking the dynamic trafficking properties of MHC molecules. T cell response analysis in…

CD4-Positive T-LymphocytesT cellRecombinant Fusion ProteinsImmunologyAntigen presentationMolecular Sequence DataMice Inbred StrainsCD8-Positive T-LymphocytesProtein Sorting SignalsMajor histocompatibility complexTransfectionViral Matrix ProteinsEpitopesMiceAntigens NeoplasmMHC class ImedicineImmunology and AllergyAnimalsHumansAmino Acid SequenceAntigensMHC class IIAntigen PresentationbiologyAntigen processingHistocompatibility Antigens Class IVaccinationMembrane ProteinsDendritic CellsMHC restrictionPhosphoproteinsCell biologyProtein Transportmedicine.anatomical_structurebiology.proteinCD8Journal of immunology (Baltimore, Md. : 1950)
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Dendritic Cells Lose Ability to Present Protein Antigen after Stimulating Antigen-Specific T Cell Responses, despite Upregulation of MHC Class II Exp…

2000

Abstract Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specialized for stimulating primary T cell responses with increased expression of MHC class II and co-stimulatory molecules, but are incapable of processing and presenting soluble protein. The current study examined whether maturation of DC is triggered by T cell recognition of antigens presented by immature DC. Human DC derived from CD34+ progenitor cells by culture with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) in serum-free medium could prime naive CD4+ T cells to keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). The cultured DC retained the abil…

CD4-Positive T-LymphocytesTime FactorsOvalbuminT cellImmunologyCD1Bone Marrow CellsCell CommunicationCulture Media Serum-FreeInterferon-gammaInterleukin 21medicineHumansImmunology and AllergyCytotoxic T cellIL-2 receptorCD40 AntigensAntigen-presenting cellCells CulturedAntigen PresentationMHC class IIbiologyInterleukin-6Tumor Necrosis Factor-alphaHistocompatibility Antigens Class IIGranulocyte-Macrophage Colony-Stimulating FactorCell DifferentiationDendritic CellsHematologyIntercellular Adhesion Molecule-1Natural killer T cellMolecular biologyCoculture Techniquesmedicine.anatomical_structureHemocyaninsB7-1 Antigenbiology.proteinImmunobiology
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Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4+ T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining T…

2019

Analysis of multifunctional CD4+ T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell receptor, and their combination with functional assays is fundamental for characterizing the antigen-specific immune response. Here we optimized a protocol for the detection of multiple intracellular cytokines within epitope-specific CD4+ T cells identified by the MHC class II tetramer technology. The optimal procedure for assessing the functional activity of tetramer-binding CD4+ T cells was based o…

CD4-Positive T-Lymphocyteslcsh:Immunologic diseases. Allergymedicine.medical_treatmentImmunologyEpitopes T-LymphocyteMajor histocompatibility complexEpitopeimmune responseMice03 medical and health sciences0302 clinical medicineImmune systemMHC-II tetramersTetramerMethodsmedicineAnimalsImmunology and Allergy030304 developmental biology0303 health sciencesMHC class IIMHC-II tetramers ICS cytokines multifunctional T cells flow cytometry immune response vaccinationStaining and LabelingbiologyChemistryflow cytometryHistocompatibility Antigens Class IIvaccinationmultifunctional T cellscytokines3. Good healthCell biologyCytokineICSbiology.proteinFemaleAntibodyPeptideslcsh:RC581-607Intracellular030215 immunologyFrontiers in Immunology
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Differential expression of alternative H2-M isoforms in B cells, dendritic cells and macrophages by proinflammatory cytokines.

1999

Major histocompatibility (MHC) class II heterodimers bind peptides generated by degradation of endocytosed antigens and display them on the surface of antigen presenting cells (APCs) for recognition by CD4+ T cells. Efficient loading of MHC class II molecules with peptides is catalyzed by the MHC class II-like molecule H2-M. The coordinate regulation of MHC class II and H2-M expression is a prerequisite for efficient MHC class II/peptide assembly in APCs determining both the generation of the T cell repertoire in the thymus and cellular immune responses in the periphery. Here we show that expression of H2-M and MHC class II genes is coordinately and cell type-specific regulated in splenic B…

CD74ImmunologyAntigen presentationGenes MHC Class IICD1Antigen-Presenting CellsGene ExpressionIn Vitro TechniquesMHC Class II GeneMiceMHC class IAnimalsProtein IsoformsMolecular BiologyDNA PrimersMHC class IIB-LymphocytesHLA-D AntigensMice Inbred BALB CbiologyBase SequenceAntigen processingHistocompatibility Antigens Class IIDendritic CellsMHC restrictionMolecular biologybiology.proteinMacrophages PeritonealCytokinesInflammation MediatorsMolecular immunology
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