Search results for "MIRNAS"
showing 9 items of 69 documents
Analysis of miRNAs in endometrial fluid as a tool for non-invasive diagnosis of endometrial receptivity
2017
La implantación embrionaria es el proceso por el que el embrión humano en fase de blastocisto se adhiere al útero materno para comenzar la gestación. El endometrio, la mucosa uterina solo puede aceptar al embrión en un momento determinado del ciclo menstrual que puede variar entre 12 horas y 2 días, identificado como "ventana de implantación". Desde siempre se ha buscado la forma de identificar de forma fiable dicha ventana utilizando técnicas histológicas, como los criterios de Noyes, a través de la observación morfológica vía ecográfica, o estudiando los cambios en los perfiles transcriptómicos, proteómicos o metabolómicos. No obstante, en reproducción asistida, se está abriendo el camino…
Interplay Between MicroRNAs and Oxidative Stress in Ovarian Conditions with a Focus on Ovarian Cancer and Endometriosis
2019
Ovarian cancer and endometriosis are two distinct gynaecological conditions that share many biological aspects incuding proliferation, invasion of surrounding tissue, inflammation, inhibition of apoptosis, deregulation of angiogenesis and the ability to spread at a distance. miRNAs are small non-coding RNAs (19–22 nt) that act as post-transcriptional modulators of gene expression and are involved in several of the aforementioned processes. In addition, a growing body of evidence supports the contribution of oxidative stress (OS) to these gynaecological diseases: increased peritoneal OS due to the decomposition of retrograde menstruation blood facilitates both endometriotic lesion developmen…
Characterization of a profile of epigenetic alterations involved in the aetiopathogenesis of endometriosis. Validation of molecular biomarkers for di…
2018
Introduction: Endometriosis is an oestrogen-dependent inflammatory disorder defined by the presence of endometrial-like tissue in ectopic locations, which limits the quality of life of affected women. This pathology affects 10% of reproductive-age women from all ethnic and social groups, although the prevalence in those patients experiencing pain, infertility or both is as high as 35%-50%, being the estimated prevalence of this condition around 176 million worldwide. Endometriosis is associated with an average diagnostic delay of 7 years, what could be partially explained by the lack of non-invasive biomarkers for diagnose, since the gold standard method is laparoscopy followed by histologi…
Binding of RNA Aptamers to Membrane Lipid Rafts: Implications for Exosomal miRNAs Transfer from Cancer to Immune Cells
2020
Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RNA and the raft-like region of the MVB membrane, or through interaction between an RBP&ndash
Association of circulating tumor DNA (ctDNA) mutations and circulating miRNAs variations, through liquid biopsy, in Non-Small Cell Lung Cancer (NSCLC…
Alterations of panoramic radiomorphometric indices in children and adolescents with beta-thalassemia major: A fractal analysis study
2021
Background Beta-thalassemia major is an inherited disorder that can cause bone deformity and loss of bone mineral density. The objective of this study is to evaluate the cortical and trabecular mandibular bone morphology of children and adolescents who have beta-thalassemia major (ß-TM) using a fractal dimension (FD) analysis and different panoramic radiomorphometric indices with digital panoramic radiographic images (DPRIs). Material and Methods The study included 80 patients (with 40 patients each of ß-TM and control). The mandibular cortical width (MCW), panoramic mandibular index (PMI), mandibular cortical index (MCI), and simple visual estimation (SVE) were evaluated, and an FD analysi…
Proof of Concept of Therapeutic Gene Modulation of MBNL1/2 in Myotonic Dystrophy
2022
La distrofia miotónica tipo 1 es una enfermedad genética rara multisistémica que afecta a 1 de cada 3000-8000 personas. La causa molecular de la enfermedad proviene de repeticiones tóxicas “CTG” en el gen DMPK (DM Protein Kinase). Tras la transcripción, estas repeticiones forman una estructura de horquilla que se une con alta afinidad a la familia de proteínas MBNL (Muscleblind-like) que agota su función de regulación de la poliadenilación y el splicing alternativo postranscripcional en numerosos transcritos. La pérdida de función de MBNL provoca una cascada de efectos posteriores, que eventualmente conducen a síntomas clínicos que incluyen miotonía, debilidad y atrofia muscular, cataratas,…
Identificación de genes y polimorfismos asociados a fenotipos óseos
2017
El deterioro óseo sucede principalmente como consecuencia del envejecimiento en ambos sexos y particularmente con la depleción estrogénica que ocurre durante la menopausia en la mujer. Este deterioro en la calidad ósea puede desembocar finalmente en el desarrollo de patologías óseas como la osteoporosis, y en última instancia en el fenotipo clínico de peor pronóstico, la fractura ósea por fragilidad. Ambos fenotipos, la osteoporosis y la fractura por fragilidad, se consideran patologías complejas o multifactoriales; esto es, influenciadas por múltiples factores entre los que se encuentran factores genéticos, epigenéticos o ambientales. En la caracterización de los factores genéticos que int…
The role of circulating and exosomal miRNAs as biomarkers of drug-resistant epilepsy
Epilepsy is estimated to affect about 65 million individuals worldwide, with up to 30 percent of drug-resistant patients who do not have remission despite appropriate therapy with antiepileptic drugs (AEDs). Therefore, it is important to distinguish drug-resistant epilepsy early in the course of disease to start a specific therapeutical approach as soon as possible. Recently circulating miRNAs have been proposed as promising biomarkers for different neurodegenerative disorders, including epilepsy. MiRNAs are a class of small non-coding RNA that regulate gene expression at a post-transcriptional level. The regulatory mechanisms controlling translation of mRNA transcripts represents to date a…