Search results for "ML"
showing 10 items of 1465 documents
Tetrazīna fragmentu saturošu linkeru sintēze proteīnu modificēšanai
2022
Maģistra darbā ir veikta jaunu, alifātisku un PEG fragmentus saturošu, tetrazīna linkeru sintēze proteīnu biokonjugācijai. Darba ietvaros iegūti un izolēti 3 linkeri, kas paredzēti neselektīvai Lys aminoskābju atlikumu modificēšanai, un arī iegūti vēl 3 linkeri, kas paredzēti reģiospecifiskai proteīna N-gala modificēšanai. Darba ietvaros arī izolēts viens linkeris, kas paredzēts His modificēšanai. Visi sintezētie linkeri tika testēti modificējot Borrelia Burgdorferi MlpC lipoproteīnu, kā arī veikta paraugu MALDI-TOF MS analīze, kurā no septiņiem linkeriem, seši ir uzrādījuši konjugāta veidošanos ar MlpC.
Generazione di modelli 3d e ricostruzione semantica su piattaforma GIS Tecniche e Metodologie
2009
Nell'ambito dei rilievi dei beni culturali è opportuno ed utile trovare sistemi alternativi ed innovativi per il rilievo, la catalogazione e la fruizione virtuale, la riproduzione materica dei modelli generati. La fase di rilievo, nel caso in esame, è stata condotta tramite l'integrazione di rilievo topografico e laser scanner finalizzato alla modellazione tridimensionale di uno dei Telamoni (dimensioni approssimative 6 mt di altezza, posto a circa 10 mt dal suolo) posti sulla Porta Nuova di Palermo ed alla successiva prototipazione. Date le difficoltà tecniche legate ad un rilievo con scanner a triangolazione ottica, per dimensioni e condizione di illuminazione, sono state realizzate scans…
Domain-specific and general future orientation of high school students in Latvia under socioeconomic changes
2013
Šio tyrimo tikslas – palyginti Latvijos paauglių bendrąją ateities orientaciją ir ateities orientacijas specifinėse gyvenimo sferose gyvenant santykinai ekonomiškai stabiliu ir ekonominės krizės laikotarpiu. Remiantis suvokimo lygio teorija, tikimasi, kad ateities orientacijos konkrečiose srityse labiau skirsis esant skirtingoms ekonominėms sąlygoms nei asmens bendroji ateities orientacija. Metodika. Tyrimas atliktas 2004 ir 2010 metais vidurinėse mokyklose. Dvi tiriamųjų grupes sudarė 530 mokinių nuo 17 iki 19 metų. P. G. Zimbardo laiko perspektyvos bei Vilčių ir baimių klausimynai latvių kalba buvo pateikti etninės daugumos atstovams, rusų kalba – etnolingvistinės mažumos atstovams. Tyrim…
Identification of the most informative wavelengths for non-invasive melanoma diagnostics in spectral region from 450 to 950 nm
2020
In this study 300 skin lesion (including 32 skin melanomas) multispectral data cubes were analyzed. The multi-step and single step machine learning approaches were analyzed to find the wavebands that provide the most information that helps discriminate skin melanoma from other benign pigmented lesions. The multi-step machine learning approach assumed training several models but proved itself to be ineffective. The reason for that is a necessity to train a segmentation model on a very small dataset and utilization of standard machine learning classifier which have shown poor classification performance. The single-step approach is based on a deep learning neural network. We have conducted 260…
PARSI
2012
A usual target of automatic assessment in CS education has been a computer program or some other software engineering product. In this paper, we describe the 'PARSI' tool that is used for the automatic assessment of stylistic and technical correctness of office documents and some basic IT skills such as email netiquette and email lists usage. The PARSI tool enables us to run a highly populated course with only one teacher, and investigate how we could make such a course a flexible learning experience where learners take an active role.
PML down-regulation in soft tissue sarcomas
2010
To date, little is known concerning the promyelocytic leukemia gene (PML) status in tumors of different origin, and its expression has never been evaluated in soft tissue sarcoma. The aim of the present study is focused on the identification of differences in terms of PML protein expression between different types of soft tissue sarcoma and the corresponding normal surrounding tissue. PML protein expression has been assessed by immunohistochemistry in six different histologic types of soft tissue sarcoma (synovial sarcoma, myofibroblastic sarcoma, angiosarcoma, liposarcoma, pleomorphic sarcoma, and leiomyosarcoma) and in the corresponding normal surrounding tissue. PML resulted significantl…
Study of the bacillus thuringiensis Cry1Ia protein oligomerization promoted by midgut brush border membrane vesicles of lepidopteran and coleopteran …
2020
Bacillus thuringiensis (Bt) produces insecticidal proteins that are either secreted during the vegetative growth phase or accumulated in the crystal inclusions (Cry proteins) in the stationary phase. Cry1I proteins share the three domain (3D) structure typical of crystal proteins but are secreted to the media early in the stationary growth phase. In the generally accepted mode of action of 3D Cry proteins (sequential binding model), the formation of an oligomer (tetramer) has been described as a major step, necessary for pore formation and subsequent toxicity. To know if this could be extended to Cry1I proteins, the formation of Cry1Ia oligomers was studied by Western blot, after the incuba…
Repeated exposure of fluazinam fungicides affects gene expression profiles yet carries no costs on a nontarget pest
2021
Fungicides are used to control pathogenic fungi of crop species, but they have also been shown to alter behavioral, life history and fitness related traits of non-target insects. Here, we tested the fungicide effects on feeding behavior, survival and physiology of the non-target pest insect, the Colorado potato beetle (Leptinotarsa decemlineata). Feeding behavior was studied by a choice test of adult beetles, which were allowed to choose between a control and a fungicide (fluazinam) treated potato leaf. Larval survival was recorded after 24-hour and 72-hour exposure to control and fungicide-treated leaves with two different concentrations. The adults did not show fungicide avoidance behavio…
Juveniilihormonin vaikutus munantuotantoon ja seksuaaliseen antagonismiin koloradonkuoriaisilla
2012
Hormonitoiminta on yksi eliöiden säätelyjärjestelmistä. Hyönteisillä keskeisessä osassa hormonisäätelyä ovat juveniilihormonit (JH). Niiden määrä yksilöiden elimistössä säätelee monia asioita yksilöiden kehityksestä lisääntymistoimintoihin. Juuri tehokas lisääntyminen onkin eliöiden tärkeimpiä tehtäviä. Hormonitoiminta pitää yllä evoluution myötä eri lajeille ja lajiryhmille kehittyneitä erilaisia lisääntymisstrategioita: jotkin lisääntyvät harvakseltaan mutta huolehtivat poikasistaan, toiset tuottavat paljon jälkeläisiä, jotka ne jättävät selviämään yksin. Sukupuolten välillä on kuitenkin eroja optimaalisessa lisääntymisstrategiassa. Naaraat joutuvat kuluttamaan jälkeläisten tuottamiseen k…
Data from: Stress for invasion success? Temperature stress of preceding generations modifies the response to insecticide stress in an invasive pest i…
2012
Adaptation to stressful environments is one important factor influencing species invasion success. Tolerance to one stress may be complicated by exposure to other stressors experienced by the preceding generations. We studied whether parental temperature stress affects tolerance to insecticide in the invasive Colorado potato beetle Leptinotarsa decemlineata. Field-collected pyrethroid-resistant beetles were reared under either stressful (17°C) or favourable (23°C) insecticide-free environments for three generations. Then, larvae were exposed to pyrethroid insecticides in common garden conditions (23°C). Beetles were in general tolerant to stress. The parental temperature stress alone affect…