Search results for "MPL"
showing 10 items of 21777 documents
Tulokkaasta tuottavaksi asiantuntijaksi : perehdyttäminen kehittämisen välineenä eräissä suomalaisissa tietoalan yrityksissä
2010
EFFETTI DELLA PROTEZIONE E DELLA COMPLESSITÀ TOPOGRAFICA SUI POPOLAMENTI BENTONICI DEI REEF A VERMETI
2009
Vermetid reef can be considered a key intertidal habitat of Mediterranean Sea and increasing knowledge about this natural system is important for its conservation. Aims of this study is to assess topography complexity, structure and composition of the benthic assemblages associated to reef formation in a marine protected area highlighting possible effects of the protection.
Montmorilonīta-antociānu organokompleksi: fizikāli ķīmiskās īpašības un izmantošanas risinājumi
2020
Bakalaura darba ietvaros tika pētīta antociānu interkalācija montmorilonīta struktūrā un veikta iegūtā organokompleksa fizikāli ķīmisko īpašību izpēte inovatīvu un videi draudzīgu materiālu izstrādē. Pētījuma ietvaros izvēlēts rūpnieciski ražots māla minerāls- montmorilonīts, kas tika modificēts ar antociāniem no melnaugļu aroniju ogu (Aronia melanocarpa L.) vai vīnogu (Vitis vinifera) izspiednēm. Antociānu sorbcija uz māla minerāla tika pētīta atkarībā no sorbenta iesvara, sorbāta koncentrācijas, vides pH un temperatūras. Iegūto kompozītmateriālu īpašības tika raksturotas ar Furjē transformācijas infrasarkanās gaismas spektroskopijas (FTIS) un termogravimetrijas analīzes (TGA) metodēm. Ieg…
Consecuencias psicológicas del maltrato en menores expuestos a violencia de género : regulación emocional, funciones ejecutivas y autoconcepto
2013
INTRODUCCIÓN Y MARCO TEÓRICO: Cada vez existe mayor evidencia de las repercusiones que comporta la exposición del maltrato en el desarrollo y salud mental de los menores, hijos e hijas de mujeres maltratadas. Es por ello que en los últimos tiempos está aumentando el interés social sobre las consecuencias psicológicas que supone estar expuesto al maltrato. Sin embargo, todavía hay pocos estudios que se dirijan a evaluar y corroborar dichos efectos en esta población. Según el modelo del Trauma Complejo, algunas situaciones traumáticas podrían producir un efecto mayor y más prolongado en la persona que lo sufre en función de algunas variables. Así, las experiencias traumáticas que se dan en la…
Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells.
2022
Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporo…
Microtubules and microfilaments in HSV-Infected rabbit-kidney cells.
1981
In rabbit kidney cells infected with strains of Herpes simplex virus producing either cell-rounding or polycaryocytosis. Vinblastine induced paracrystals. This could be shown by phase-contrast- and electron-microscopy. Infections were done under one-step-growth conditions or at low MOI. 90 per cent noninfected cells contained stress fibers as detected by Servablue R250-staining. Shortly after recruitment into polycaryocytes, stress fibres of normal length appearing in criss-cross arrangement can be seen in the periphery of these cells. Later they polymerize to very long fibers and finally they are partially destroyed. The time of destruction depends on the MOI employed. By using Actinomycin…
Vaginal infection of mice with HSV type 2 variant ER−: A new animal model for human primary genital HSV type 2 infections
1992
Abstract Studying the pathogenesis of vaginal infections in mice with two variants of Herpes simplex virus type 2 (HSV-2) strain ER we observed that both variants ER+ and ER− caused severe vaginitis but only ER+ invaded the CNS leading to lethal neurological disease. In contrast, mice infected with ER− cleared the virus from the vagina and recovered from infection. ER+ and ER− expressed equal levels of thymidine kinase (TK) indicating a TK-independent difference in neurovirulence. Using the non-neurovirulent variant ER−, we were able to investigate humoral immune responses late after infection. Vaginal infection with ER− suppressed serum antibody formation after a secondary systemic HSV-1 i…
The endogenous retroviral insertion in the human complement C4 gene modulates the expression of homologous genes by antisense inhibition
2001
Intron 9 contains the complete endogenous retrovirus HERV-K(C4) as a 6.4-kb insertion in 60% of human C4 genes. The retroviral insertion is in reverse orientation to the C4 coding sequence. Therefore, expression of C4 could lead to the transcription of an antisense RNA, which might protect against exogenous retroviral infections. To test this hypothesis, open reading frames from the HERV sequence were subcloned in sense orientiation into a vector allowing expression of a beta-galactosidase fusion protein. Mouse L cells which had been stably transfected with either the human C4A or C4B gene both carrying the HERV insertion (LC4 cells), and L(Tk-) cells without the C4 gene were transiently tr…
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
2021
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …
Replication of herpes simplex virus type 1 and 2 in the medulla of the adrenal gland after vaginal infection of mice.
1996
After vaginal infections of mice with neuroinvasive strains of herpes simplex virus type 1 and 2 (HSV-1, HSV-2) virus replicates in the epithelium of the vagina, in the paravaginal ganglia, in the spinal cord and finally in the brain and in the adrenal glands. However, viral antigens could be demonstrated only in the medulla of the adrenal glands but not in the cortex, as assessed by immunohistochemistry (IHC). HSV could not be isolated from liver, spleen, uterus, and ovaries. This contrasts to the intraperitoneal (i.p) route of infection with replication in different visceral organs including the adrenal gland's cortex.