Search results for "MR"

showing 10 items of 3001 documents

Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons

2011

Transport of mRNAs to diverse neuronal locations via RNA granules serves an important function in regulating protein synthesis within restricted sub-cellular domains. We recently detected the Huntington's disease protein huntingtin (Htt) in dendritic RNA granules; however, the functional significance of this localization is not known. Here we report that Htt and the huntingtin-associated protein 1 (HAP1) are co-localized with the microtubule motor proteins, the KIF5A kinesin and dynein, during dendritic transport of β-actin mRNA. Live cell imaging demonstrated that β-actin mRNA is associated with Htt, HAP1, and dynein intermediate chain in cultured neurons. Reduction in the levels of Htt, H…

congenital hereditary and neonatal diseases and abnormalitiesHuntingtinDyneinModels NeurologicalBiological Transport ActiveKinesinsRNA-binding proteinNerve Tissue Proteinsmacromolecular substancesBiologyCytoplasmic GranulesMicrotubulesArticle03 medical and health sciences0302 clinical medicineMicrotubulemental disordersProtein biosynthesisMRNA transportAnimalsRNA MessengerRNA Small InterferingRats WistarCells Cultured030304 developmental biologyNeurons0303 health sciencesHuntingtin ProteinMultidisciplinaryMolecular Motor ProteinsBrainDyneinsNuclear ProteinsRNA-Binding ProteinsDendritesActinsCell biologynervous system diseasesRatsDendritic transportnervous systemGene Knockdown TechniquesKinesinFemale030217 neurology & neurosurgerySignal TransductionScientific Reports
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Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk

2019

Web spiders connect silk proteins, so-called spidroins, into fibers of extraordinary toughness. The spidroin N-terminal domain (NTD) plays a pivotal role in this process: it polymerizes spidroins through a complex mechanism of dimerization. Here we analyze sequences of spidroin NTDs and find an unusually high content of the amino acid methionine. We simultaneously mutate all methionines present in the hydrophobic core of a spidroin NTD from a nursery web spider’s dragline silk to leucine. The mutated NTD is strongly stabilized and folds at the theoretical speed limit. The structure of the mutant is preserved, yet its ability to dimerize is substantially impaired. We find that side chains of…

congenital hereditary and neonatal diseases and abnormalitiesProtein Foldinggenetic structuresProtein ConformationScienceSilkmacromolecular substancesCircular dichroismcomplex mixturesArticleMethionineddc:590ddc:570AnimalsAmino Acid Sequencelcsh:ScienceFluorescence spectroscopySequence Homology Amino AcidfungiQtechnology industry and agricultureSpidersSpectrometry FluorescenceMutationThermodynamicslcsh:QProtein MultimerizationFibroinsSolution-state NMRHydrophobic and Hydrophilic InteractionsAlgorithmsNature Communications
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Incorporation of Pt(II) complex with [amino-2(methylthio)(1,2,4)triazole-(1,5-a)pyrimidine-6-carboxylic-acid] ligand in MCM41 for controlled release

2015

Drug carriers play a critical role for the loading and the release of the drug. A promising frontier is represented by a new class of innovative medicines that represents directional transport vehicles "drug delivery" and consist of assembled structures carrier (nano)-drug. Silica-based materials, nontoxic, biocompatible, have been used as adjuvant and excipient in pharmaceutical technology. In this class of compounds, the mesoporous materials, such as MCM41, SBA-15 and hexagonal mesoporous silica, have been investigated for medication and drug delivery due to their properties. In fact, these materials show a large specific pore volume made up of regular pores having a diameter in the nanom…

controlled release MCM41 platinum(II) complexes 29Si {1H} CP-MAS NMR.
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Teacher smiles as an interactional and pedagogical resource in the classroom

2020

In classroom settings, laughter and smiles are resources for action that are available to both teachers and students. Recent interactional studies have documented how students use these resources to deal with trouble of various kind, but less is known about the sequential and activity contexts of teachers’ laughter-relevant practices, as well as their pedagogical functions. We use multimodal conversation analysis (CA) to investigate the interactional unfolding and pedagogical orientations of teacher smiles during instructional IRE (initiation-response-evaluation) sequences in a corpus of 37 bilingual lessons collected in schools in Finland and Spain. In analysing the focal smiles, we pay at…

conversation analysisConversation analysisAprendizaje integrado de contenido e idiomas (CLIL)hymyAnálisis de conversacionesluokkatyöskentelySmileAnàlisi de conversesnaurucontent and language integrated learning (CLIL)opettaja-oppilassuhdeAprenentatge integrat de contingut i idiomes (CLIL)keskustelunanalyysismiling37SonrisaRiureopetustilanneLaughvieraskielinen opetusSomriureInteraction in the classroomInteracción en el aula risaclassroom interactionlaughterInteracció a l'aulaIntegrated content and language learning (CLIL)
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On the mechanistic behavior of highly efficient palladium-tetraphosphine catalytic systems for cross-coupling reactions: first spectroscopic and elec…

2008

Electrochemical studies carried out in conjunction with 31P NMR spectroscopy on the palladium(II)/palladium(0) halogeno complexes of the tetraphosphine 1,1′,2,2′-tetrakis(diphenylphosphino)-4,4′-di...

crystal structureDenticityredox reactionoxidative additiontetraphosphinechemistry.chemical_elementmechanism010402 general chemistryElectrochemistryPhotochemistry01 natural sciencesCoupling reactionCatalysisInorganic Chemistry[ CHIM.CATA ] Chemical Sciences/Catalysiscross-coupling reactionsferrocenylpolyphosphine complexesPhysical and Theoretical ChemistryComputingMilieux_MISCELLANEOUSelectrochemical studies010405 organic chemistryChemistryOrganic Chemistry[CHIM.CATA]Chemical Sciences/CatalysispalladiumCombinatorial chemistryOxidative addition0104 chemical sciences3. Good healthcatalytic systemsreaction kinetics31p nmr spectroscopyPalladium
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Crystal structure and Hirshfeld surface analysis of 4-allyl-2-meth­oxy-6-nitro­phenol

2020

The crystal structure of 4-allyl-2-meth­oxy-6-nitro­phenol, which crystallizes in the centrosymmetric space group P with three independent mol­ecules in the asymmetric unit, is reported along with the Hirshfeld surface analysis.

crystal structureStackingCrystal structure010402 general chemistry01 natural sciencesResearch Communicationschemistry.chemical_compoundNitric acidNitrationHirshfeld surface analysisGeneral Materials SciencenitroeugenolCrystallography010405 organic chemistryHydrogen bondAromaticityGeneral ChemistryCondensed Matter PhysicsNMR0104 chemical sciencesCrystallographynitro­eugenolchemistryQD901-999hydrogen bondsNitroIRHydroxideActa Crystallographica Section E: Crystallographic Communications
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Mononuclear rearrangements of heterocycles in water/β-CD: information on the real site of reaction from structural modifications of substrates and fr…

2007

Abstract The effect of β-cyclodextrin (β-CD) on the reactivity in the base-catalyzed pathway for the rearrangement in water of some ( Z )-hydrazones of 3-benzoyl-1,2,4-oxadiazoles ( 1b – f ) into the relevant triazoles ( 2b – f ) was investigated, finding different behavior as a function of the proton concentration. ESIMS and 1 H NMR data evidence the formation of host – guest complexes. The whole of the experimental and calculated (MM2) data enabled us to draw some intriguing conclusions concerning the influence of the structures of the substrates and the nature of the formed host – guest complexes on the real site of the reaction.

cyclodextrinProtonConcentration dependenceStereochemistryChemistryElectrospray ionizationOrganic ChemistryDrug DiscoveryProton NMR124-oxadiazoleReactivity (chemistry)mononuclear rearrangementBiochemistryTetrahedron
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Effects of a progressive aquatic resistance exercise program on the biomechanical composition and morphology of cartilage in women with mild knee ost…

2013

Background. Symptoms associated with osteoarthritis of the knee result in decreased function, loss of working capacity and extensive social and medical costs. There is a need to investigate and develop effective interventions to minimise the impact of and even prevent the progression of osteoarthritis. Aquatic exercise has been shown to be effective at reducing the impact of osteoarthritis. The purpose of this article is to describe the rationale, design and intervention of a study investigating the effect of an aquatic resistance exercise intervention on cartilage in postmenopausal women with mild knee osteoarthritis. Methods. A minimum of 80 volunteers who meet the inclusion criteria will…

dGEMRICAquatic exerciseOsteoarthritisluuQuantitative MRIT2 relaxation time
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Tärähdyksiä sisältävän harjoittelun vaikutus polvinivelrustoon ja toimintakykyyn lievää polvinivelrikkoa sairastavilla 50-65 -vuotiailla naisilla : s…

2016

Nivelrikko on etenkin ikääntyneeseen väestöön kohdistuva kipuja ja toiminnallisia rajoituksia aiheuttava nivelsairaus. Tutkimuksen tarkoituksena oli selvittää liikunnan vaikutuksia polvinivelruston laatuun ja seurata mahdollisia rustomuutoksia 12 kuukauden kuluttua harjoittelun lopettamisesta lievää polvinivelrikkoa sairastavilla 50-65 –vuotiailla naisilla. Lisäksi tarkoituksena oli tutkia harjoittelun vaikutuksia fyysiseen toimintakykyyn ja polven oireisiin sekä arvioida vaikutusten pysyvyyttä intervention jälkeen. Tutkimukseen osallistui 61 vapaaehtoista naista, joilla oli lievä radiologinen polven nivelrikko. He kaikki osallistuivat 12 kuukauden interventiojaksoon ja sitä seuranneeseen 1…

dGEMRICnivelrikkoT2tärähdyksiä sisältävä harjoittelunivelrustofyysinen toimintakykykollageenitharjoitteluglykosaminoglykaanibiokemiallinen koostumuspolvilumpio
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Problēmrisināšanas uzdevumi dabaszinību apguvē 4.-6. klasē

2022

Pieredzes darbs “Problēmrisināšanas uzdevumi dabaszinību apguvē 4.-6. klasē” ir pieredzē balstīts darbs, kura mērķis bija apkopot informāciju par problēmrisināšanas uzdevumu lietošanu un izstrādāt uzdevumu piemērus dabaszinību apguvei pamatskolā. Darba izstrāde notika vispārizglītojošas skolas dabaszinību stundās trīs 4. klasēs, vienā 5. klasē un vienā 6. klasē. Rezultātā tika izstrādāti un aprobēti trīs tematu plāni dabaszinību apguvei 4., 5. un 6. klasē, stundu plāni, metodiskie līdzekļi un vērtēšanas metodika problēmrisināšnas uzdevumam tematos “Gaisma” (4. klase), “Dabas resursi” (5. klase) un “Šūna, audi, organisms”(6. klase).

dabaszinību apguveIzglītības zinātnespamatskolaaktīva mācīšanāsmetodikas aprobācijaproblēmrisināšanas uzdevumi
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