Search results for "MTO"

showing 10 items of 538 documents

Inducible NO synthase confers chemoresistance in head and neck cancer by modulating survivin

2009

The dual role of the inducible NO synthase (iNOS) and NO signaling in head and neck squamous cell carcinoma (HNSCC) is a complex and can both promote or inhibit tumor progression. However, the underlying molecular mechanisms are not yet resolved in detail. We show for the first time that conditions, favoring low NO levels conferred resistance against cisplatin/taxol-induced apoptosis in HNSCC cell lines. Cytoprotection was mediated by survivin, because we observed its upregulation subsequent to low doses of the NO donors S-nitroso-N-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) or ectopic expression of physiologic amounts of iNOS. Also, RNAi-mediated depletion of survivin block…

MaleUmbilical VeinsCancer ResearchSurvivinFluorescent Antibody TechniqueNitric Oxide Synthase Type IIApoptosisp38 Mitogen-Activated Protein KinasesInhibitor of Apoptosis ProteinsImmunoenzyme TechniquesPhosphatidylinositol 3-Kinaseschemistry.chemical_compoundLY294002Enzyme InhibitorsRNA Small InterferingAged 80 and overReverse Transcriptase Polymerase Chain ReactionCell CycleMiddle AgedCell cycleOncologyHead and Neck NeoplasmsCarcinoma Squamous CellFemaleMicrotubule-Associated ProteinsNitroprussidePaclitaxelImmunoblottingAntineoplastic AgentsS-Nitroso-N-AcetylpenicillamineBiologyCell LineDownregulation and upregulationSurvivinmedicineHumansNitric Oxide DonorsRNA MessengerneoplasmsProtein kinase BNitritesPI3K/AKT/mTOR pathwayAgedmedicine.diseaseAntineoplastic Agents PhytogenicHead and neck squamous-cell carcinomachemistryDrug Resistance NeoplasmTumor progressionImmunologyCancer researchEndothelium VascularCisplatinProto-Oncogene Proteins c-aktInternational Journal of Cancer
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Role of mTOR-regulated autophagy in spine pruning defects and memory impairments induced by binge-like ethanol treatment in adolescent mice.

2021

Abstract Adolescence is a brain maturation developmental period during which remodeling and changes in synaptic plasticity and neural connectivity take place in some brain regions. Different mechanism participates in adolescent brain maturation, including autophagy that plays a role in synaptic development and plasticity. Alcohol is a neurotoxic compound and its abuse in adolescence induces neuroinflammation, synaptic and myelin alterations, neural damage and behavioral impairments. Changes in synaptic plasticity and its regulation by mTOR have also been suggested to play a role in the behavioral dysfunction of binge ethanol drinking in adolescence. Therefore, by considering the critical ro…

MaleautophagyDendritic spineSynaptic pruningPeriod (gene)synaptic pruningBiologyPathology and Forensic MedicineBinge Drinkingbinge ethanol treatmentMyelinMicemedicineAnimalsPI3K/AKT/mTOR pathwayNeuroinflammationResearch Articlescognitive functionMemory DisordersNeuronal PlasticityGeneral NeuroscienceTOR Serine-Threonine KinasesAutophagyBraindendritic spinesMice Inbred C57BLmedicine.anatomical_structureSynaptic plasticitymTORFemaleadolescenceNeurology (clinical)NeuroscienceResearch ArticleBrain pathology (Zurich, Switzerland)
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Altered REDD1, myostatin, and Akt/mTOR/FoxO/MAPK signaling in streptozotocin-induced diabetic muscle atrophy

2011

Type 1 diabetes, if poorly controlled, leads to skeletal muscle atrophy, decreasing the quality of life. We aimed to search highly responsive genes in diabetic muscle atrophy in a common diabetes model and to further characterize associated signaling pathways. Mice were killed 1, 3, or 5 wk after streptozotocin or control. Gene expression of calf muscles was analyzed using microarray and protein signaling with Western blotting. We identified translational repressor protein REDD1 (regulated in development and DNA damage responses) that increased seven- to eightfold and was associated with muscle atrophy in diabetes. The diabetes-induced increase in REDD1 was confirmed at the protein level. …

Malemedicine.medical_specialtyMAP Kinase Signaling SystemPhysiologyEndocrinology Diabetes and MetabolismFOXO1P70-S6 Kinase 1MyostatinBiologyMiceRandom Allocation03 medical and health sciences0302 clinical medicinePhysiology (medical)Internal medicinemedicineAnimalsRNA MessengerPhosphorylationMuscle SkeletalProtein kinase BPI3K/AKT/mTOR pathwayOligonucleotide Array Sequence Analysis030304 developmental biology0303 health sciencesForkhead Box Protein O1Gene Expression ProfilingTOR Serine-Threonine KinasesUbiquitinationForkhead Transcription FactorsOrgan SizeMyostatinProtein ubiquitinationMuscle atrophyMuscular AtrophyDNA Repair EnzymesDiabetes Mellitus Type 1EndocrinologyGene Expression Regulationbiology.proteinPhosphorylationmedicine.symptomProto-Oncogene Proteins c-akt030217 neurology & neurosurgeryTranscription FactorsAmerican Journal of Physiology-Endocrinology and Metabolism
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Cannabinoid receptor 1 and acute resistance exercise – In vivo and in vitro studies in human skeletal muscle

2015

Abstract Aim This study aimed to determine whether Cannabinoid receptor 1 (CB1) is involved in mammalian target of rapamycin (mTOR) signaling and skeletal muscle protein synthesis. Methods This study used human vastus lateralis skeletal muscle biopsies obtained before and after a resistance exercise (RE) bout in young men (n = 18). The signaling mechanisms were studied in vitro in human myotubes. Protein expression was determined by Western blot and confocal microscopy, and gene expression by quantitative PCR. Protein synthesis was measured in vitro using puromycin-based SuNSET technique. Results In human skeletal muscle, an anabolic stimulus in the form of RE down-regulated CB1 expression.…

Malemedicine.medical_specialtyPhysiologyMAP Kinase Signaling SystemMuscle Fibers SkeletalGene ExpressionSkeletal muscleP70-S6 Kinase 1Cell Cycle ProteinsBiochemistryCell LineCellular and Molecular NeuroscienceYoung AdultEndocrinologyPiperidinesReceptor Cannabinoid CB1Internal medicinemedicineCannabinoid receptor type 2HumansCannabinoid receptor 1PhosphorylationMuscle Skeletalta315PI3K/AKT/mTOR pathwayAdaptor Proteins Signal TransducingChemistryMyogenesista1184Eukaryotic initiation factor 4E bindingSkeletal muscleRibosomal Protein S6 Kinases 70-kDaResistance TrainingPhosphoproteinsResistance exerciseCell biologymedicine.anatomical_structureEndocrinologyRibosomal protein s6Protein BiosynthesismTOR signalingPhosphorylationPyrazolesProtein synthesisProtein Processing Post-TranslationalPeptides
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DNA Methylation in Inflammatory Pathways Modifies the Association between BMI and Adult-Onset Non-Atopic Asthma

2019

A high body mass (BMI) index has repeatedly been associated with non-atopic asthma, but the biological mechanism linking obesity to asthma is still poorly understood. We aimed to test the hypothesis that inflammation and/or innate immunity plays a role in the obesity-asthma link. DNA methylome was measured in blood samples of 61 non-atopic participants with asthma and 146 non-atopic participants without asthma (non-smokers for at least 10 years) taking part in the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in Adults (SAPALDIA) study. Modification by DNA methylation of the association of BMI or BMI change over 10 years with adult-onset asthma was examined at each CpG sit…

MaleobesityNon-atopic asthmaHealth Toxicology and Mutagenesislcsh:MedicineToxicologyBody Mass IndexCohort StudiesMice0302 clinical medicineMedicineinnate immunitynon-atopic asthmaInnate immunity0303 health sciencesDNA methylationNF-kappa Bepigenome-wide association study3. Good healthCpG siteDNA methylationFemaleEpigeneticsmedicine.symptomGlucocorticoidmedicine.drugAdultMAP Kinase Signaling SystemInflammationArticle03 medical and health sciencesEpigenome-wide association studyMD MultidisciplinaryAnimalsHumansObesityEpigeneticsadult-onset asthmaPI3K/AKT/mTOR pathway030304 developmental biologyAsthmaInflammationepigeneticsbusiness.industrylcsh:RPublic Health Environmental and Occupational Healthmedicine.diseaseObesityAsthmarespiratory tract diseasesPPAR gamma030228 respiratory systeminflammationImmunologybusinessAdult-onset asthmaInternational Journal of Environmental Research and Public Health
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Charge injection and trapping at perovskite interfaces with organic hole transporting materials of different ionization energies

2019

The extraction of photogenerated holes from CH3NH3PbI3 is crucial in perovskite solar cells. Understanding the main parameters that influence this process is essential to design materials and devices with improved efficiency. A series of vacuum deposited hole transporting materials (HTMs) of different ionization energies, used in efficient photovoltaic devices, are studied here by means of femtosecond transient absorption spectroscopy. We find that ultrafast charge injection from the perovskite into the different HTMs (<100 fs) competes with carrier thermalization and occurs independently of their ionization energy. Our results prove that injection takes place from hot states in the valence…

Materials science530 Physicslcsh:Biotechnology02 engineering and technologyElectronTrapping7. Clean energy01 natural sciencesVacuum depositionlcsh:TP248.13-248.65540 Chemistry0103 physical sciencesUltrafast laser spectroscopyGeneral Materials ScienceMaterialsPerovskite (structure)010302 applied physicsGeneral EngineeringCarrier lifetime021001 nanoscience & nanotechnologylcsh:QC1-999SemiconductorsChemical physicsFemtosecondIonization energy0210 nano-technologylcsh:PhysicsAPL Materials
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Photo-induced electron transfer at nanostructured semiconductor–zinc porphyrin interface

2014

Abstract Electron transfer at metal oxide–organic dye interface on ZnO nanorod (ZnOr) templates was studied by femtosecond absorption spectroscopy method. Further confirmation of the electron transfer was obtained from photoelectrical studies. The fastest electron transfer from zinc porphyrin (ZnP) to semiconductor was observed for ZnOr modified by a 5 nm layer of TiO2 (

Materials scienceAbsorption spectroscopybusiness.industryGeneral Physics and AstronomyPhotochemistryMetalZinc porphyrinElectron transferSemiconductorvisual_artFemtosecondvisual_art.visual_art_mediumNanorodPhysical and Theoretical Chemistrybusinessta116Layer (electronics)Chemical Physics Letters
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Transient absorption with a femtosecond tunable excitation pump reveals the emission kinetics of color centers in amorphous silica.

2021

We report a set of femtosecond (fs) transient absorption (TA) measurements following the dynamics of the so-called nonbridging oxygen hole center in silica, a model color center in wide bandgap amorphous solids, characterized by a very large Stokes shift between the UV excitation and its associated red emission at 1.9 eV. The changes in the TA spectrum were probed in the UV-visible range at various delays after photoexcitation and analyzed as a function of the UV excitation energy, in single-photon absorption conditions. The combination of the experiments helps to clarify the defect photocycle, highlighting how TA measurements with tunable UV excitation could represent a powerful tool to in…

Materials scienceAbsorption spectroscopytransient absorption02 engineering and technology01 natural sciencesMolecular physics010309 opticssymbols.namesakeOpticsStokes shift0103 physical sciencesUltrafast laser spectroscopyluminescenceAbsorption (electromagnetic radiation)ultra-fast laser spectroscopyComputingMilieux_MISCELLANEOUS[PHYS.PHYS]Physics [physics]/Physics [physics]business.industrypoint defect021001 nanoscience & nanotechnologyAtomic and Molecular Physics and OpticsAmorphous solidPhotoexcitationsilicaFemtosecondsymbols0210 nano-technologybusinessExcitationOptics letters
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Laser irradiation of carbon–tungsten materials

2014

Carbon–tungsten layers deposited on graphite by thermionic vacuum arc (TVA) were directly irradiated with a femtosecond terawatt laser. The morphological and structural changes produced in the irradiated area by different numbers of pulses were systematically explored, both along the spots and in their depths. Although micro-Raman and Synchrotron-x-ray diffraction investigations have shown no carbide formation, they have shown the unexpected presence of embedded nano-diamonds in the areas irradiated with high fluencies. Scanning electron microscopy images show a cumulative effect of the laser pulses on the morphology through the ablation process. The micro-Raman spatial mapping signalled an…

Materials scienceAcoustics and UltrasonicsScanning electron microscopeAnalytical chemistrychemistry.chemical_elementVacuum arcTungstenCondensed Matter PhysicsLaserSurfaces Coatings and FilmsElectronic Optical and Magnetic Materialslaw.inventionCarbideX-ray photoelectron spectroscopychemistrylawFemtosecondIrradiationJournal of Physics D: Applied Physics
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Picosecond energy relaxation in

2005

Abstract Investigating the reflectance response dynamics of La 0.67 Ca 0.33 MnO 3 thin films after excitation by femtosecond laser pulses, we identify for the first time a picosecond relaxation step which only exists below the Curie temperature T C . The relaxation time increases from zero at T C to several picoseconds at low temperatures. The data can be explained with the existence of a magnetization-related effective energy gap, and assuming relaxation between these states to be mediated by a Frohlich-type electron–lattice interaction.

Materials scienceCondensed matter physicsBand gapRelaxation (NMR)Condensed Matter PhysicsLaserElectronic Optical and Magnetic Materialslaw.inventionCondensed Matter::Materials ScienceMagnetizationlawPicosecondFemtosecondCurie temperatureCondensed Matter::Strongly Correlated ElectronsElectrical and Electronic EngineeringExcitationPhysica B: Condensed Matter
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