Search results for "Maize"
showing 10 items of 59 documents
Commercial thickeners used by patients with dysphagia: Rheological and structural behaviour in different food matrices
2015
In order to achieve a safe swallowing in patients with dysphagia, liquids must be thickened. In this work, two commercial starch based thickeners dissolved inwater,whole milk, apple juice and tomato juice were studied. The thickeners were Resource®, composed of modified maize starch and Nutilis®, composed of modified maize starch and gums. They were formulated at two different concentrations corresponding to nectar- and pudding-like consistencies. Influence of composition, concentration and food matrix on rheological properties and structure of the resulting pastes were analysed. Viscoelastic measurements and microscopic observations of the thickeners dissolved in water revealed structural …
Modelling of Starch Gelatinisation and Liquefaction with the Enzyme Liquozyme
2011
In this paper the influence of the enzyme Liquozyme Supra concentration (0.4-1.2 kg/t dry substance DS) and gelatinisation time (3-11 min.) on the liquefied starch quality is investigated. The composition in sugars with different Degrees of Polymerisation (DP) as DP1 (glucose), DP2 (maltose), DP3 (maltotriose), DP4 (maltotetrose) and oligosaccharides with DP≥5 is analysed by using HPLC with RI detector. The products obtained have different DP, the main components being oligosaccharides with DP≥5, followed by small amount of DP3+DP4 and much smaller concentrations of DP1; no DP2 is produced by this enzyme. Two quality criteria: the percent of oligosaccharides with DP≥5 and the percent of DP1…
Transcription of genes in the biosynthetic pathway for fumonisin mycotoxins is epigenetically and differentially regulated in the fungal maize pathog…
2012
ABSTRACT When the fungal pathogen Gibberella moniliformis (anamorph, Fusarium verticillioides ) colonizes maize and maize-based products, it produces class B fumonisin (FB) mycotoxins, which are a significant threat to human and animal health. FB biosynthetic enzymes and accessory proteins are encoded by a set of clustered and cotranscribed genes collectively named FUM, whose molecular regulation is beginning to be unraveled by researchers. FB accumulation correlates with the amount of transcripts from the key FUM genes, FUM1 , FUM21 , and FUM8 . In fungi in general, gene expression is often partially controlled at the chromatin level in secondary metabolism; when this is the case, the deac…
Determination of ochratoxin A in maize bread samples by LC with fluorescence detection.
2007
Ochratoxin A (OTA) is a secondary fungal metabolite produced by several moulds, mainly by Aspergillus ochraceus, A. carbonarius, A. niger and by Penicillium verrucosum. The present work shows the results of comparative studies using different procedures for the analysis of OTA in maize bread samples. The studied analytical methods involved extraction with different volumes of PBS/methanol, different extraction apparatus, and clean-up through immunoaffinity columns. The separation and identification were carried out by high-performance liquid chromatography with fluorescence detection. The optimized method for analysis of OTA in maize bread involved extraction with PBS:methanol (50:50), and …
Zagrożenie upraw kukurydzy szkodnikiem Diabrotica virgifera (w oparciu o monitoring prowadzony na obszarze wybranych gmin województwa opolskiego)
2018
Powiększający się z roku na rok areał kukurydzy w Polsce, stwarza idealne warunki do rozwoju wielu agrofagów. Do najgroźniejszych z nich należy zachodnia kukurydziana stonka korzeniowa Diabrotica virgifera Le Conte, która z powodu specyficznego sposobu żerowania powoduje bardzo duże straty plonu. Do 6 lutego 2014 roku stonka posiadała status organizmu kwarantannowego i inwazyjnego na terenie całej Unii Europejskiej, w tym także w Polsce. Ocenę zagrożenia upraw przez szkodnika, przeprowadzono w latach 2012-2013 na obszarze dwóch miejscowości Zopowy i Lubotyń (gminy Głubczyce i Kietrz), a następnie porównano je z badaniami monitoringowymi prowadzonymi przez Państwową Inspekcję Ochrony Roślin …
Cross-resistance and mechanism of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia n…
2011
The cross-resistance spectrum and biochemical mechanism of resistance to the Bacillus thuringiensis Cry1Ab toxin was studied in a field-derived strain of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) that was further selected in the laboratory for high levels (>1000-fold) of resistance to Cry1Ab. The resistant strain exhibited high levels of cross-resistance to Cry1Ac and Cry1Aa but only low levels of cross-resistance (<4-fold) to Cry1F. In addition, there was no significant difference between the levels of resistance to full-length and trypsin-activated Cry1Ab protein. No differences in activity of luminal gut proteases or altered proteolytic processing of the toxin were observed in…
Fusaproliferin, beauvericin and enniatins: occurrence in food – a review
2012
The present review summarises data on the prevalence and concentration of fusaproliferin, beauvericin and enniatins in small grains, maize and processed grain-based foods. These fungal secondary metabolites possess interesting biological activities and are presently often referred to as 'emerging mycotoxins'. They have been considered as less important since they are likely not of acute toxicity. However, their high prevalence in foodstuffs, occasionally in higher mg/kg concentrations, warrants an assessment of their true importance for food (and feed) safety. Thorough surveys that determine the contamination of raw and processed foods with fusaproliferin, beauvericin and enniatins are sti…
Projekts: Maizes ceptuves SIA "Riekstiņi" dibināšana
2021
Maģistra darba mērķis ir izstrādāt projektu maizes ceptuves SIA “Riekstiņi” dibināšanai Latvijā, Mārupes novadā. Projekta ideja – nodibināt SIA un atvērt nelielu maizes ceptuvi, ar mērķi ražot maizi un maizes izstrādājumus. Maizes ražošana paredzētā no dabīga ierauga, jo šāda veida maize būtiski papildina veselīgu uzturu. Pēc darba autores domām ideja par dabīgi raudzēto maizi ir ļoti aktuāla, jo mūsdienās cilvēki aktīvi rūpējas par savu veselību, cenšas lietot ēšanā sabalansētu uzturu un EKO (dabīgus) produktus. Tādēļ ir svarīgi paredzēt veselīga produkta ražošanu. Par uzņēmuma komercdarbības formu darba autore izvēlējas SIA, pamatojoties uz šādiem kritērijiem: 1.Visatbilstošākais pamatkap…
Spatial Distribution of Fungal Communities in an Arable Soil.
2015
Fungi are prominent drivers of ecological processes in soils, so that fungal communities across different soil ecosystems have been well investigated. However, for arable soils taxonomically resolved fine-scale studies including vertical itemization of fungal communities are still missing. Here, we combined a cloning/Sanger sequencing approach of the ITS/LSU region as marker for general fungi and of the partial SSU region for arbuscular mycorrhizal fungi (AMF) to characterize the microbiome in different maize soil habitats. Four compartments were analyzed over two annual cycles 2009 and 2010: a) ploughed soil in 0-10 cm, b) rooted soil in 40-50 cm, c) root-free soil in 60-70 cm soil depth a…
Isolation and purification of enniatins A, A1, B, B1, produced by Fusarium tricinctum in solid culture, and cytotoxicity effects on Caco-2 cells
2010
Enniatins (ENs) are antibiotic compounds of hexadepsipeptidic structure produced by several strains of Fusarium spp. The ENs A, A(1), B, B-1 were purified from extracts of Fusarium tricinctum grown on a solid medium of corn, by a low pressure liquid chromatography (LPLC) on reverse phase of Amberlite XAD-7 followed by semipreparative LC. The purity and the structure of the isolated compounds were confirmed by LC-MS/MS. The technique of the purification of the fungal extract enabled complete separation of the ENs A, A(1), B, B-1 with a mean purity of 97% for all the compounds. The cytoxicity of the ENs was tested in the cell lines of human origin (epithelial colorectal adenocarcinoma cells, …