Search results for "Marr"

showing 10 items of 776 documents

Therapy-related acute myeloid leukemia developing 14 years after allogeneic hematopoietic stem cell transplantation, from a persistent R882H- DNMT3A …

2018

Abstract Background Therapy-related acute myeloid leukemia (t-AML) develops in patients with prior exposure to cytotoxic therapies. Selection of a pre-existing TP53 mutated clone prone to acquire additional mutational events has been suggested as the main pathogenic mechanism of t-AML. Here, we report a unique case of t-AML which developed from a pre-existing DNMT3A mutated clone that persisted in the patient for more than 10 years despite treatment with intensive chemotherapy and allogeneic hematopoietic stem cell transplantation (alloHSCT). Case presentation A 42-year-old male was diagnosed with AML harboring a normal karyotype and mutations in the NPM1 (c.863_864ins, p.W288 fs*12), DNMT3…

AdultMaleOncologymedicine.medical_specialtyNPM1Allogeneic transplantationmedicine.medical_treatmentClinical BiochemistryMutation MissenseClone (cell biology)Therapy-Related Acute Myeloid LeukemiaHematopoietic stem cell transplantationDNA Methyltransferase 3APathology and Forensic Medicine03 medical and health sciences0302 clinical medicineInternal medicinemedicineHumansTransplantation HomologousDNA (Cytosine-5-)-MethyltransferasesMolecular BiologyBone Marrow Transplantationbusiness.industryMyeloid leukemiaInduction chemotherapyTransplantationLeukemia Myeloid Acute030220 oncology & carcinogenesisbusinessNucleophosmin030215 immunologyExperimental and Molecular Pathology
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Bone quality in the midpalate for temporary anchorage devices.

2009

Objective The aim of the present study was to quantitatively assess the bone quality of the palatal bone from an implantologic standpoint. Material and methods The material consisted of palatal tissue blocks of autopsy material from 22 subjects (19 male, three female), between 18 and 63 years of age. The specimens comprised the anterior part of median palate (APMP) from about 7 mm behind the incisive foramen (first premolar regions), the middle part of median palate (MPMP, second premolar region), and the posterior part of the median palate (PPMP, first molar region). They were prepared in the transversal plane using ground-thin-section technology. The midpalatal areas of the different part…

AdultMalePalate HardYounger ageBone densityAdolescentDentistryHard tissueMandibular first molarYoung AdultBone DensityBone Marrowparasitic diseasesBone qualityPremolarmedicineCadaverOrthodontic Anchorage ProceduresHumansIncisive foramenDental Implantsbusiness.industryAge FactorsCranial SuturesMiddle Agedmedicine.anatomical_structureFemaleOral SurgerybusinessBone volumeClinical oral implants research
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Bone marrow biopsy in hemophagocytic syndrome.

2002

Abstract Aims. Hemophagocytic syndrome (HPS) is a severe and acute clinical event occurring with fever, hepatosplenomegaly, and pancytopenia due to uncontrolled phagocytosis of blood cells and precursors. Although HPS represents a secondary phenomenon, it can mask the underlying condition, generally a neoplastic or infective disease, thus making the patient management rather difficult. The aims of this study were to point out the main pathological features useful to highlight the primary disease and show the eventual discrepancies among the different cases. Methods and results. Bone-marrow biopsies (BMBs) of 26 patients with HPS were morphologically and immunophenotypically evaluated; the p…

AdultMalePathologymedicine.medical_specialtyAdolescentHistiocytosis Non-Langerhans-CellBiopsyT-LymphocytesHepatosplenomegalyPathology and Forensic MedicineBone MarrowBiopsymedicineBiomarkers TumorHumansMolecular BiologyHistiocyteAgedAged 80 and overmedicine.diagnostic_testbusiness.industryHistiocytesCell BiologyGeneral MedicineHyperplasiaMiddle Agedmedicine.diseasePancytopeniaImmunohistochemistryHistiocytosisLeukemiaVirus DiseasesHematologic NeoplasmsHemophagocytosismedicine.symptombusinessVirchows Archiv : an international journal of pathology
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Type 3 innate lymphoid cells producing IL-17 and IL-22 are expanded in the gut, in the peripheral blood, synovial fluid and bone marrow of patients w…

2015

Background The aim of the study was to better characterise the immunological origin and the behaviour of interleukin (IL)-23-responsive innate lymphoid cells (ILCs) in the gut, synovial fluid (SF) and bone marrow (BM) of patients with ankylosing spondylitis (AS).Methods ILC1, ILC2 and ILC3 cells were determined and characterised by confocal microscopy and flow cytometry in ileal and BM biopsies, in peripheral blood (PB) and SF mononuclear cells obtained from patients with AS and controls. Mucosal vascular addressin cell adhesion molecule 1 (MADCAM-1), IL-7, IL-15 and aggregates of lymphoid tissue inducer cells (LTi) were evaluated by immunohistochemistry. The in vitro ability of epithelial …

AdultMalePathologymedicine.medical_specialtyAdolescentImmunologyHigh endothelial venulesImmunoglobulinsPeripheral blood mononuclear cellGeneral Biochemistry Genetics and Molecular BiologyInterleukin 22Young AdultMucoproteinsAnkylosing Spondylitis; Cytokines; InflammationRheumatologyBone MarrowIleumSynovial FluidAddressinImmunology and AllergyMedicineSynovial fluidHumansSpondylitis AnkylosingLymphocytesIntestinal MucosaCytokineAgedInterleukin-15InflammationMicroscopy ConfocalAnkylosing SpondylitibiologyNatural Cytotoxicity Triggering Receptor 2business.industryInterleukin-7InterleukinsInnate lymphoid cellInterleukin-17Middle AgedSettore MED/16 - Reumatologiamedicine.anatomical_structureLymphatic systemCase-Control Studiesbiology.proteinFemaleBone marrowbusinessCell Adhesion Molecules
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Significance of increased blastic-appearing cells in bone marrow following myeloablative unrelated cord blood transplantation in adult patients.

2012

An abnormal increase of nonleukemic blastic-appearing lymphocytes in bone marrow (BM) specimens has been reported after unrelated cord blood transplantation (UCBT). This study analyzed the incidence, chronology, biological features, and clinical significance of elevated numbers of these cells in a series of 165 consecutive adult patients demonstrating myeloid engraftment after myeloablative UCBT in a single institution. The patients' BM samples were routinely evaluated by cytomorphology at different time points after UCBT. When ≥5% of blastic-appearing cells were detected by cytomorphology in the BM, samples were also evaluated by multiparametric flow cytometry to characterize these cells. …

AdultMalePathologymedicine.medical_specialtyAllogeneic Hematopoietic stem Cell TransplantMyeloidBone Marrow CellsTransplantation ChimeraCord Blood Stem Cell TransplantationHematogonesFlow cytometryInmune ReconstitutionBone MarrowmedicineHumansClinical significanceCumulative incidenceLymphocyte CountTransplantationTransplantation Chimeramedicine.diagnostic_testbusiness.industryIncidence (epidemiology)HematologyFetal BloodPrognosismedicine.anatomical_structureFemaleBone marrowCord Blood Stem Cell TransplantationbusinessBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
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Morphometric Study of the Bone Marrow in Polycythemia Vera Following Interferon-Alpha Therapy

1993

Bone marrow cellularity and extent of fibrotic change were determined in nineteen patients with polycythemia vera, treated with interferon-alpha (IFN) for 1 year. The cellularity was evaluated with an interactive semiautomatic method using Leitz TAS plus microscope: in particular, number and size of megakaryocytes were evaluated after immunostaining with Y2/51 (CD 61); reticulin content was studied by light microscope with a semiquantitative method. Before IFN therapy mean cellularity was 80.5% (+/- 13.7). After 6 and 12 months mean cellularity was 75.4% and 68.4% respectively. Six months after cessation of IFN therapy the cellularity was 69.1%. A decrease of the number, density and morphom…

AdultMalePathologymedicine.medical_specialtyAlpha interferonCell CountPathology and Forensic MedicinePolycythemia veraBone MarrowFibrosishemic and lymphatic diseasesmedicineHumansMyelofibrosisPolycythemia VeraAgedAged 80 and overbusiness.industryInterferon-alphaCell BiologyMiddle Agedmedicine.diseaseBone marrow cellularitymedicine.anatomical_structurePrimary MyelofibrosisMarrow fibrosisFemaleBone marrowbusinessMegakaryocytesImmunostainingPathology - Research and Practice
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Different immunophenotypical apoptotic profiles characterise megakaryocytes of essential thrombocythaemia and primary myelofibrosis.

2009

Aims: Essential thrombocythaemia (ET) and primary myelofibrosis (PMF) share some clinical and pathological features, but show different biological behaviour and prognosis. The latest contributions to understanding the nature of these disorders have focused on bone marrow microenvironment remodelling and proliferative stress, recognising megakaryocytes (MKCs) as “key-cells”. The aim of this study was to investigate the apoptotic profile of ET and PMF MKCs in order to further characterise the biology of these disorders. Methods: Bone marrow biopsy samples from 30 patients with ET, and 30 patients with PMF, were immunophenotypically studied for the expression of pro-apoptotic (Fas, Fas-L, Bax,…

AdultMalePathologymedicine.medical_specialtyBiopsyIDIOPATHIC MYELOFIBROSISApoptosisPOLYCYTHEMIA-VERASettore MED/08 - Anatomia PatologicaBiologyPathology and Forensic MedicineImmunophenotypingImmunophenotypingMegakaryocyteBone MarrowmedicineIn Situ Nick-End LabelingHumansTelomerase reverse transcriptaseMyelofibrosisMOLECULAR PERSPECTIVEAgedAged 80 and overTUNEL assayEssential thrombocythemiaC-MPLMUTATION STATUSGeneral MedicineMiddle Agedmedicine.diseasePrognosismedicine.anatomical_structureCHRONIC MYELOPROLIFERATIVE DISORDERSCELL-DEATHApoptosisPrimary MyelofibrosisRISK-FACTORSCancer researchBONE-MARROW ANGIOGENESISMYELOID METAPLASIAFemaleBone marrowMegakaryocytesThrombocythemia EssentialJournal of clinical pathology
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Detection of residual leukemic blasts in adult patients with acute T-lymphoblastic leukemia using bone marrow trephine biopsies: comparison of fluore…

2010

Evaluation of remission in adult acute lymphoblastic leukemia (ALL) normally relies on cytologic evaluation and flow-cytometric analysis. The diagnostic impact of bone marrow trephine biopsies has not been studied so far. We investigated 26 biopsies of 16 patients with T-ALL. Double fluorescent immunostaining with TdT and CD3 was performed. Corresponding cytologic evaluation and flow-cytometric data were available. In 17 of 26 investigations, the results were concordant (either positive or negative). In one examination, residual blasts were not recognized by trephine biopsy, but by other methods. By contrast, in eight investigations, the leukemic blasts were found only in the bone marrow tr…

AdultMalePathologymedicine.medical_specialtyCD3BiopsyCytological TechniquesFluorescent Antibody TechniqueCell SeparationPrecursor T-Cell Lymphoblastic Leukemia-LymphomaSensitivity and SpecificityPathology and Forensic MedicineFlow cytometryYoung AdultBone MarrowCytologyBiopsyMedicineHumansmedicine.diagnostic_testbiologybusiness.industryCell BiologyMiddle AgedFlow CytometryMinimal residual diseaseAdult Acute Lymphoblastic Leukemiabiology.proteinImmunohistochemistryFemalebusinessImmunostainingPathology, research and practice
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Intrasinusoidal bone marrow infiltration: a possible hallmark of splenic lymphoma.

1996

A particular type of lymphomatous bone marrow infiltration defined as intrasinusoidal and occuring in seven patients with splenomegaly and mild to moderate thrombocytopenia is reported. Immunocytochemistry highlighted the intrasinusoidal pattern and showed a mature B-cell phenotype. Four patients underwent splenectomy and a diagnosis of splenic marginal cell lymphoma was made. The disease course seems to be chronic and quite indolent. All patients are alive and well ; follow-up ranges from 2 to 42 months. The value of bone marrow biopsy as a primary diagnostic tool in splenic marginal zone lymphomas is discussed.

AdultMalePathologymedicine.medical_specialtyHistologyLymphoma B-Cellmedicine.medical_treatmentSplenectomySpleenPathology and Forensic MedicineImmunophenotypingSinusoidBone MarrowBiopsymedicineHumansAgedmedicine.diagnostic_testbusiness.industryLymphoma Non-HodgkinSplenic NeoplasmsBone Marrow ExaminationGeneral MedicineMiddle Agedmedicine.diseaseImmunohistochemistrymedicine.anatomical_structureImmunohistochemistryFemaleBone marrowSplenic diseaseSplenic LymphomabusinessHistopathology
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CD27 distinguishes two phases in bone marrow infiltration of splenic marginal zone lymphoma.

2004

Aims:  To investigate CD27 expression in splenic marginal zone lymphoma (SMZL), an indolent low-grade B-cell lymphoma with constant involvement of the bone marrow, especially with an intrasinusoidal pattern. It is not clear if the neoplastic clone is composed of virgin or somatically mutated B cells. CD27 is reported to be a hallmark of memory B cells. Methods and results:  We evaluated 64 bone marrow biopsy specimens (BMBs) from 36 patients with SMZL for the expression of CD27. For comparison, splenectomy specimens of patients with traumatic splenic rupture or with SMZL were used. All BMBs showed lymphomatous infiltration. When located in the marrow sinusoids, neoplastic cells were CD27– i…

AdultMalePathologymedicine.medical_specialtyHistologybone marrowLymphomaBiopsyNaive B cellsplenic marginal zone lymphomaSplenic NeoplasmSpleenPathology and Forensic MedicineBiopsyintrasinusoidal infiltrationmedicineHumansSplenic marginal zone lymphomaCD27naive and memory B cellsAgedAged 80 and overmedicine.diagnostic_testbusiness.industrySplenic NeoplasmsGeneral MedicineMiddle Agedmedicine.diseaseBone Marrow NeoplasmImmunohistochemistryTumor Necrosis Factor Receptor Superfamily Member 7bone marrow; CD27; intrasinusoidal infiltration; naive and memory B cells; splenic marginal zone lymphomamedicine.anatomical_structureBone marrow neoplasmFemaleBone marrowbusinessBone Marrow NeoplasmsInfiltration (medical)
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