Search results for "Membrane microdomains"

showing 3 items of 63 documents

Binding of RNA Aptamers to Membrane Lipid Rafts: Implications for Exosomal miRNAs Transfer from Cancer to Immune Cells

2020

Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RNA and the raft-like region of the MVB membrane, or through interaction between an RBP&ndash

liposomesendocrine systemmacromolecular substancesexosomesArticleCatalysisraftslcsh:ChemistryInorganic ChemistryMembrane LipidsMembrane Microdomainsimmune cellsCell Line TumorNeoplasmsmicroRNAHumansRNA aptamersPhysical and Theoretical Chemistrylcsh:QH301-705.5Molecular BiologyLipid raftSpectroscopyChemistrySELEXMacrophagesVesicleCell MembraneOrganic ChemistryMultivesicular BodiesRNA-Binding ProteinsRNADendritic CellsGeneral MedicineRaftAptamers NucleotideMicrovesiclesComputer Science ApplicationsCell biologyKiller Cells NaturalMicroRNAslcsh:Biology (General)lcsh:QD1-999Cancer cellmiRNAslipids (amino acids peptides and proteins)Systematic evolution of ligands by exponential enrichmentInternational Journal of Molecular Sciences
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Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry

2013

ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.

virusesImmunologyEndocytic cycleTetraspanin 24EndocytosisMicrobiologyClathrinDynamin IIPolymerizationDynamin IIMembrane MicrodomainsTetraspaninVirologyCaveolinHumansHuman papillomavirus 31Lipid raftDynaminHuman papillomavirus 16Microscopy ConfocalHuman papillomavirus 18biologyvirus diseasesLysosome-Associated Membrane GlycoproteinsVirus InternalizationVirologyActinsEndocytosisVirus-Cell InteractionsCell biologyMicroscopy ElectronMicroscopy FluorescenceInsect Sciencebiology.proteinElectrophoresis Polyacrylamide GelHeLa CellsJournal of Virology
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Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)

2008

BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…

viruseslcsh:MedicinePlatelet Membrane GlycoproteinsTetraspanin 24CaveolaeKidneyEndocytosisClathrinVirusCell LineMembrane MicrodomainsViral life cycleTetraspaninAntigens CDCaveolaeInfectious Diseases/Viral InfectionsCaveolinInfectious Diseases/Sexually Transmitted DiseasesHumanslcsh:ScienceHuman papillomavirus 16MultidisciplinarybiologyTetraspanin 30lcsh:RVirionMembrane Proteinsvirus diseasesCell BiologyVirus InternalizationVirology/Host Invasion and Cell EntryVirologyClathrinEndocytosisCell biologyCell culturebiology.proteinFemalelcsh:QMicrobiology/Cellular Microbiology and PathogenesisHeLa CellsResearch ArticlePLoS ONE
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