Search results for "MerA"

showing 10 items of 3426 documents

Numeral classifiers in East Asia

2017

AbstractThe understanding of the functional range of numeral classifiers requires a combined effort from different theoretical approaches and the integration of findings from syntax, semantics and pragmatics. This article demonstrates how such an integrative perspective creates new insights into classifiers in Sinitic languages in the fields of counting and word order, the expression of definiteness and indefiniteness and the syntax of verbal classification.

060201 languages & linguisticsNumeral systemLinguistics and LanguageDefinitenessComputer science0602 languages and literatureTheoretical linguisticsIdentifiabilityEast Asia06 humanities and the artsLanguage and LinguisticsLinguisticsWord orderLinguistics
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Primera persona del plural en los juicios orales. Valor representativo y estrategia atenuante

2018

El objetivo de este artículo es doble, pues nos proponemos estudiar los valores que puede desarrollar la primera persona del plural en los juicios orales y, en relación con ello, examinar si es posible identificar usos atenuantes asociados a este mecanismo referencial. Tras analizar nuestro corpus siguiendo una metodología cualitativa y cuantitativa, hemos podido observar que el uso de la primera persona del plural en este género no solo se debe al carácter representativo del sistema legal español o a las imposiciones de la tradición discursiva. Frecuentemente, el uso de la primera persona del plural (especialmente el plural de modestia y el plural sociativo) codifica un valor atenuante ori…

060201 languages & linguisticsValue (ethics)Linguistics and LanguageLiterature and Literary Theorymedia_common.quotation_subjectQuantitative methodology06 humanities and the artsPragmaticsLanguage and LinguisticsLinguisticsatenuaciónmitigationNegotiationjuicios oralesfirst person pluraloral trialsFirst person0602 languages and literatureprimera persona del pluralSociologypragmáticapragmaticsPluralmedia_commonRilce. Revista de Filología Hispánica
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Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme

2018

Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP…

10207 Department of AnthropologyArtificial Gene Amplification and ExtensionGene mutationPathology and Laboratory MedicineFrance/epidemiologyBiochemistryPolymerase Chain Reactionlaw.inventionSwitzerland/epidemiologylcsh:SciencePhylogenyMammalsBacterialEukaryotaGeneral MedicineMacrolides/pharmacologyMultilocus Sequence Typing/methods3. Good healthBacterial PathogensNucleic acidsMedical MicrobiologyLeporidsMacrolidesAlleles; Anti-Bacterial Agents/pharmacology; DNA Bacterial/genetics; France/epidemiology; Genome Bacterial; Genotype; Globus Pallidus; Macrolides/pharmacology; Multilocus Sequence Typing/methods; Phylogeny; Polymorphism Single Nucleotide; RNA Ribosomal 23S/genetics; Sequence Analysis DNA/methods; Switzerland/epidemiology; Syphilis/epidemiology; Treponema pallidum/geneticsGeneral Agricultural and Biological SciencesSwitzerlandGenotypeSequence analysis030106 microbiologySexually Transmitted Diseases1100 General Agricultural and Biological SciencesGlobus PallidusMicrobiologyAnti-Bacterial Agents/pharmacology10127 Institute of Evolutionary Biology and Environmental Studies03 medical and health sciences1300 General Biochemistry Genetics and Molecular Biology23S ribosomal RNAGeneticsTypingSyphilisPolymorphismNon-coding RNAMolecular Biology TechniquesMicrobial PathogensMolecular BiologyAllelesRibosomal1000 MultidisciplinaryGenitourinary Infectionslcsh:ROrganismsBiology and Life SciencesDNATropical DiseasesTreponema pallidum/genetics030104 developmental biologyGenetic LociGeneral Biochemistrylcsh:QMultilocus Sequence Typing0301 basic medicineBacterial DiseasesBacterial/geneticslcsh:MedicineTreponematosesGeographical LocationslawGenotypeMedicine and Health Sciences23S/geneticsTreponema PallidumPolymerase chain reactionGeneticsMultidisciplinaryTreponemaGenome10177 Dermatology ClinicSingle NucleotideAnimal Models10218 Institute of Legal MedicineAnti-Bacterial AgentsDNA/methodsEuropeRNA Ribosomal 23SInfectious DiseasesRibosomal RNAExperimental Organism SystemsVertebratesFranceRabbitsPathogensSequence AnalysisResearch ArticleNeglected Tropical DiseasesDNA BacterialCell biologyCellular structures and organellesUrology610 Medicine & healthGenetics and Molecular BiologyBiologyResearch and Analysis MethodsPolymorphism Single NucleotideAnimalsEuropean Unionddc:613Syphilis/epidemiologySequence Analysis DNAbiology.organism_classificationddc:616.8People and PlacesAmniotesMultilocus sequence typingRNARibosomesGenome BacterialPLoS ONE
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Stereochemical features of the hydrolysis of 9,10-epoxystearic acid catalysed by plant and mammalian epoxide hydrolases

2002

cis-9,10-Epoxystearic acid was used as a tool to probe the active sites of epoxide hydrolases (EHs) of mammalian and plant origin. We have compared the stereochemical features of the hydrolysis of this substrate catalysed by soluble and membrane-bound rat liver EHs, by soluble EH (purified to apparent homogeneity) obtained from maize seedlings or celeriac roots, and by recombinant soybean EH expressed in yeast. Plant EHs were found to differ in their enantioselectivity, i.e. their ability to discriminate between the two enantiomers of 9,10-epoxystearic acid. For example, while the maize enzyme hydrated both enantiomers at the same rate, the EH from soybean exhibited very high enantioselecti…

1303 BiochemistryStereochemistryMolecular Sequence DataDiol10050 Institute of Pharmacology and Toxicology610 Medicine & healthPolymerase Chain ReactionBiochemistrySubstrate Specificity1307 Cell BiologyHydrolysischemistry.chemical_compound1312 Molecular BiologyAnimalsOrganic chemistryMolecular BiologyDNA PrimersEpoxide HydrolasesMammalschemistry.chemical_classificationBase SequencebiologyChemistryHydrolysisFatty acidActive siteStereoisomerismCell BiologyPlantsRecombinant ProteinsRatsKineticsLiverMicrosomal epoxide hydrolaseEpoxide Hydrolasesbiology.protein570 Life sciences; biologyStereoselectivitySoybeansEnantiomerStearic AcidsResearch Article
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Metabolomic Study of Dactylis glomerata Growing on Aeolian Archipelago (Italy)

2022

The Aeolian Islands (Italy) are a volcanic archipelago in the Tyrrhenian Sea comprising seven main islands, among which are two active volcanoes. The peculiar geological features and the wide variety of environments and soils have an important impact on native plants, and in particular, the Aeolian populations of Dactylis glomerata (a perennial cool-season bunchgrass) exhibit remarkable phenotypic variability. Considering that environmental drivers also strongly affect the production of plant metabolites, this work aimed at comparing the metabolomic profiles of D. glomerata (leaves) harvested at different altitudes on four islands of the Aeolian archipelago, namely: Lipari, Vulcano, Strombo…

1H NMR metabolomicEndocrinology Diabetes and MetabolismAeolian IslandDactylis glomerataMolecular BiologyBiochemistryplant metabolites<i>Dactylis glomerata</i>; Aeolian Islands; <sup>1</sup>H NMR metabolomics; plant metabolitesMetabolites
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Real-life evaluation of a rapid extraction-free SARS-CoV-2 RT-PCR assay (COVID-19 PCR Fast-L) for the diagnosis of COVID-19.

2021

2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)SARS-CoV-2Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Extraction (chemistry)COVID-19BiologyVirologyPolymerase Chain ReactionSensitivity and SpecificityInfectious DiseasesReal-time polymerase chain reactionLife evaluationVirologyCOVID-19 Nucleic Acid TestingHumansRNA ViralProspective StudiesLetter to the EditorJournal of medical virology
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Repeated Testing in SARS-CoV-2 Infection

2020

2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Pneumonia ViralTestingReal-Time Polymerase Chain ReactionSevere Acute Respiratory SyndromeArticleBetacoronavirusRepeated testingCOVID-19 TestingPandemicHumansMedicinePandemicsbiologyClinical Laboratory Techniquesbusiness.industrySARS-CoV-2COVID-19General Medicinebiology.organism_classificationTesting SARS-CoV-2 COVID-19VirologyCoronavirus InfectionsbusinessBetacoronavirusCoronavirus InfectionsMayo Clinic Proceedings
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Zinc(II)—The Overlooked Éminence Grise of Chloroquine’s Fight against COVID-19?

2020

The authors would like to thank Agnieszka Michalczuk for providing us with her artistic vision of SARS-CoV-2.

2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)lcsh:Medicinelcsh:RS1-441Pharmaceutical Sciencechemistry.chemical_elementZincReviewlcsh:Pharmacy and materia medicachloroquine03 medical and health scienceschemistry.chemical_compoundChloroquineRNA polymeraseDrug Discoverymedicine030304 developmental biology0303 health sciences030306 microbiologybusiness.industrySARS-CoV-2lcsh:RCOVID-19HydroxychloroquineChloroquinehydroxy- chloroquineVirologychemistry2019-nCoVMolecular MedicinebusinessZn(II) ionophoresmedicine.drugHydroxychloroquinePharmaceuticals
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Clinical utility of novel biosensing platform: Diagnosis of coronavirus SARS-CoV-2 at point of care

2021

Early detection is the first step in the fight against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, an efficient, rapid, selective, specific, and inexpensive SARS-CoV-2 diagnostic method is the need of the hour. The reverse transcription-polymerase chain reaction (RT-PCR) technology is massively utilized to detect infection with SARS-CoV-2. However, scientists continue to strive to create enhanced technology while continually developing nanomaterial-enabled biosensing methods that can provide new methodologies, potentially fulfilling the present demand for rapid and early identification of coronavirus disease 2019 (COVID-19) patients. Our review presents a summar…

2019-20 coronavirus outbreakmedicine.medical_specialtyDiagnostic methodsMaterials scienceCoronavirus disease 2019 (COVID-19)SARS-CoV-2virusesMechanical EngineeringSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)fungiEarly detectionCondensed Matter PhysicsArticlePolymerase chain reactionBiosensors02 Physical Sciences 03 Chemical Sciences 09 EngineeringMechanics of MaterialsmedicineGeneral Materials ScienceCOVID-19 clinical diagnosticsSevere acute respiratory syndrome coronavirusIntensive care medicineMaterialsNanomaterialsPoint of careMaterials Letters
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Vision metrology and Structure from Motion for archaeological heritage 3D reconstruction: A Case Study of various Roman mosaics

2017

&lt;p class="Abstract"&gt;Vision metrology and computer vision can be successfully used for archaeological heritage 3D reconstruction in very high precision 3D measurement projects. Of those archaeological objects requiring very accurate measurements (&amp;lt;1 mm), ancient mosaics comprise some of the most important. The aim of this paper is to assess the photogrammetric/computer vision approach in a vision metrology context as part of a 3D mosaics survey. In order to evaluate the optimal photogrammetric/computer vision workflow in this work, three different surveys were performed on three mosaics of different sizes and locations. Two of these are stored at the &lt;em&gt;Antonino Salinas&l…

3D modelContext (archaeology)Mechanical Engineering3D reconstructionCamera calibration3D model; Accuracy; Camera calibration; Computer vision; Mosaic; Photogrammetry;PhotogrammetryDocumentationGeographyWorkflowPhotogrammetryStructure from motionComputer visionElectrical and Electronic EngineeringScale (map)MosaicInstrumentationCartographyAccuracySettore ICAR/06 - Topografia E CartografiaCamera resectioning
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