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RESEARCH PRODUCT
Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme
Angèle Gayet-ageronTristan FerryMatthias CavassiniMichal StrouhalStephan LautenschlagerPhilipp P. BosshardP SednaouiFabrizio DutlyKay NieseltNatasha AroraLenka MikalováDavid ŠMajsMarta Pla-díazTanika BawaHomayoun C. BagheriLinda GrillováFernando González-candelasMichael Krützensubject
10207 Department of AnthropologyArtificial Gene Amplification and ExtensionGene mutationPathology and Laboratory MedicineFrance/epidemiologyBiochemistryPolymerase Chain Reactionlaw.inventionSwitzerland/epidemiologylcsh:SciencePhylogenyMammalsBacterialEukaryotaGeneral MedicineMacrolides/pharmacologyMultilocus Sequence Typing/methods3. Good healthBacterial PathogensNucleic acidsMedical MicrobiologyLeporidsMacrolidesAlleles; Anti-Bacterial Agents/pharmacology; DNA Bacterial/genetics; France/epidemiology; Genome Bacterial; Genotype; Globus Pallidus; Macrolides/pharmacology; Multilocus Sequence Typing/methods; Phylogeny; Polymorphism Single Nucleotide; RNA Ribosomal 23S/genetics; Sequence Analysis DNA/methods; Switzerland/epidemiology; Syphilis/epidemiology; Treponema pallidum/geneticsGeneral Agricultural and Biological SciencesSwitzerlandGenotypeSequence analysis030106 microbiologySexually Transmitted Diseases1100 General Agricultural and Biological SciencesGlobus PallidusMicrobiologyAnti-Bacterial Agents/pharmacology10127 Institute of Evolutionary Biology and Environmental Studies03 medical and health sciences1300 General Biochemistry Genetics and Molecular Biology23S ribosomal RNAGeneticsTypingSyphilisPolymorphismNon-coding RNAMolecular Biology TechniquesMicrobial PathogensMolecular BiologyAllelesRibosomal1000 MultidisciplinaryGenitourinary Infectionslcsh:ROrganismsBiology and Life SciencesDNATropical DiseasesTreponema pallidum/genetics030104 developmental biologyGenetic LociGeneral Biochemistrylcsh:QMultilocus Sequence Typing0301 basic medicineBacterial DiseasesBacterial/geneticslcsh:MedicineTreponematosesGeographical LocationslawGenotypeMedicine and Health Sciences23S/geneticsTreponema PallidumPolymerase chain reactionGeneticsMultidisciplinaryTreponemaGenome10177 Dermatology ClinicSingle NucleotideAnimal Models10218 Institute of Legal MedicineAnti-Bacterial AgentsDNA/methodsEuropeRNA Ribosomal 23SInfectious DiseasesRibosomal RNAExperimental Organism SystemsVertebratesFranceRabbitsPathogensSequence AnalysisResearch ArticleNeglected Tropical DiseasesDNA BacterialCell biologyCellular structures and organellesUrology610 Medicine & healthGenetics and Molecular BiologyBiologyResearch and Analysis MethodsPolymorphism Single NucleotideAnimalsEuropean Unionddc:613Syphilis/epidemiologySequence Analysis DNAbiology.organism_classificationddc:616.8People and PlacesAmniotesMultilocus sequence typingRNARibosomesGenome Bacterialdescription
Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 | PLoS ONE |