Search results for "Method"
showing 10 items of 13253 documents
Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4+ T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining T…
2019
Analysis of multifunctional CD4+ T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell receptor, and their combination with functional assays is fundamental for characterizing the antigen-specific immune response. Here we optimized a protocol for the detection of multiple intracellular cytokines within epitope-specific CD4+ T cells identified by the MHC class II tetramer technology. The optimal procedure for assessing the functional activity of tetramer-binding CD4+ T cells was based o…
Transverse-momentum-dependent Multiplicities of Charged Hadrons in Muon-Deuteron Deep Inelastic Scattering
2017
A semi-inclusive measurement of charged hadron multiplicities in deep inelastic muon scattering off an isoscalar target was performed using data collected by the COMPASS Collaboration at CERN. The following kinematic domain is covered by the data: photon virtuality $Q^{2}>1$ (GeV/$c$)$^2$, invariant mass of the hadronic system $W > 5$ GeV/$c^2$, Bjorken scaling variable in the range $0.003 < x < 0.4$, fraction of the virtual photon energy carried by the hadron in the range $0.2 < z < 0.8$, square of the hadron transverse momentum with respect to the virtual photon direction in the range 0.02 (GeV/$c)^2 < P_{\rm{hT}}^{2} < 3$ (GeV/$c$)$^2$. The multiplicities are pres…
Tensile behaviour of anti-symmetric CFRP composite
2011
Abstract This paper addresses the response of a 17-ply anti-symmetric carbon/epoxy composite subjected to uniaxial tensile loading. Hashin ply damage model is adopted to describe the damage behaviour of the plies, whereas damage initiation and progression of the interfaces are characterised by mixed-mode cohesive damage model. Force-displacement curves obtained numerically and experimentally show good agreement. Results show that all laminae and interfaces experience the damage except laminae with 0o fibre. In addition, damage is concentrated at the tab and central regions of the tensile specimen. Edge delamination is observed in all interfaces.
ALICE forward rapidity upgrades
2017
Two forward rapidity upgrades of the ALICE apparatus at the LHC are presented: the Muon Forward Tracker (MFT) and the Fast Interaction Trigger (FIT). Their designs are driven by physics performance studies, which have been done using MC simulations. Several prototypes have been tested. Currently the detector design phase has ended. The final layouts, geometries and expected performance figures are presented. The latest developments from the test of the prototypes are discussed. The MFT will improve the performance of the ALICE Muon Spectrometer by adding vertexing capabilities to the system. The elementary component of the MFT is a Monolithic Active Pixel Sensor (MAPS), using the TowerJazz …
Perspectives for CNO neutrino detection in Borexino
2018
International audience; Borexino measured with unprecedented accuracy the fluxes of solar neutrinos emitted at all the steps of the pp fusion chain. Still missing is the measurement of the flux of neutrinos produced in the CNO cycle. A positive measurement of the CNO neutrino flux is of fundamental importance for understanding the evolution of stars and addressing the unresolved controversy on the solar abundances. The measurement of the CNO neutrino flux in Borexino is challenging because of the low intensity of this component (CNO cycle accounts for about 1% of the energy emitted by Sun), the lack of prominent spectral features and the presence of background sources. The main background c…
Stress Transfer within CNT Fibres: A FEA Approach
2015
Abstract Carbon nanotube (CNT) fibres are characterized by extreme anisotropy in their structure and physical properties. These fibres have been shown to have high axial strength, but poor shear strength between carbon nanotubes; for this reason it is difficult to transfer stress uniformly acrossthe fibre cross section. Here, Finite Element Analysis (FEA) is used to predict the stress distribution and the stress-strain curves of CNT fibres. The resultsdemonstrate that, in accordance with St. Venant principle,very considerable length-to-diameter ratios (> 10 3 ) are required to obtain a uniform stress distribution within the fibres even in the presence of low applied strain.
Measurement of the lifetime of tau-lepton
1996
The tau lepton lifetime is measured with the L3 detector at LEP using the complete data taken at centre-of-mass energies around the Z pole resulting in tau_tau = 293.2 +/- 2.0 (stat) +/- 1.5 (syst) fs. The comparison of this result with the muon lifetime supports lepton universality of the weak charged current at the level of six per mille. Assuming lepton universality, the value of the strong coupling constant, alpha_s is found to be alpha_s(m_tau^2) = 0.319 +/- 0.015(exp.) +/- 0.014 (theory). The tau lepton lifetime is measured with the L3 detector at LEP using the complete data taken at centre-of-mass energies around the Z pole resulting in τ τ =293.2 ± 2.0 (stat) ± 1.5 (syst) fs . The c…
Correlated gluonic hot spots meet symmetric cumulants data at LHC energies
2019
We present a systematic study on the influence of spatial correlations between the proton constituents, in our case gluonic hot spots, their size and their number on the symmetric cumulant SC(2, 3), at the eccentricity level, within a Monte Carlo Glauber framework [1]. When modeling the proton as composed by 3 gluonic hot spots, the most common assumption in the literature, we find that the inclusion of spatial correlations is indispensable to reproduce the negative sign of SC(2, 3) in the highest centrality bins as dictated by data. Further, the subtle interplay between the different scales of the problem is discussed. To conclude, the possibility of feeding a 2+1D viscous hydrodynamic sim…
Late Breaking Abstract - Validation of a tool for the assessment of disease awareness (DA) in COPD
2017
Background: DA could influence adherence to treatment and outcomes. No validated tools are available to assess DA in COPD Aims: To generate a tool able to explore DA in COPD patients, the Disease Awareness in COPD Questionnaire (DACQ) Methods: A 2-step Delphi approach was used to develop a questionnaire that was tested for validity in a cohort of COPD patients of an Italian observational, multicenter, prospective study (SAT study - NCT02689492) Results: Consensus on a 27-item provisional questionnaire was obtained with 2 Delphi rounds. Factor analysis showed a 4-factor solution accounting for 35% of variance; each factor (disease acceptance, awareness of treatment needs, disease knowledge, …
<p>Early management of COPD: where are we now and where do we go from here? A Delphi consensus project</p>
2019
Purpose There is a lack of consensus on the most appropriate early diagnostic strategy, criteria for early access to treatment and follow-up approach for patients with COPD. Materials and methods A Delphi consensus project investigated the early management of COPD. We formulated two questionnaires for completion by pneumologists in Italy. Results A total of 207 specialists completed questionnaire 1 and 184 of them questionnaire 2, between November 2016 and October 2017. Early diagnosis of COPD was considered uncommon for 93.2% of the expert panel. Regardless of the definition of "early diagnosis" - a diagnosis made before the clinical manifestation of the disease for most responders (60.4%)…