Search results for "Methylocystaceae"

showing 3 items of 3 documents

Isolation of methanotrophic bacteria from termite gut.

2015

The guts of termites feature suitable conditions for methane oxidizing bacteria (MOB) with their permanent production of CH4 and constant supply of O2 via tracheae. In this study, we have isolated MOB from the gut contents of the termites Incisitermes marginipennis, Mastotermes darwiniensis, and Neotermes castaneus for the first time. The existence of MOB was indicated by detecting pmoA, the gene for the particulate methane monooxygenase, in the DNA of gut contents. Fluorescence in situ hybridization and quantitative real-time polymerase chain reaction supported those findings. The MOB cell titer was determined to be 10(2)-10(3) per gut. Analyses of the 16S rDNA from isolates indicated clos…

DNA Bacterialfood.ingredientMethane monooxygenaseColony Count MicrobialIsopteraReal-Time Polymerase Chain ReactionMicrobiologyMethylococcaceaeDNA RibosomalMicrobiologyfoodMastotermes darwiniensisRNA Ribosomal 16SAnimalsIn Situ Hybridization FluorescenceMethylocystis bryophilabiologyBacteriaSequence Analysis DNAbiology.organism_classificationGastrointestinal TractMicroscopy FluorescenceMethylocystaceaeMethylocystisMethylococcaceaebiology.proteinOxygenasesMethylocystis parvusMethaneMethylocystaceaeBacteriaMicrobiological research
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Genome Sequence of the Methanotrophic Poly-β-Hydroxybutyrate Producer Methylocystis parvus OBBP

2012

-- PAGS 2 5709-5710

DNA Bacterialfood.ingredientOperonMethane monooxygenasePolyestersMolecular Sequence DataMethylocystisHydroxybutyratesmonooxigenasaMicrobiologyfoodmethylotrophOperonBotanyMolecular BiologyGeneGeneticsWhole genome sequencingbiologySequence Analysis DNAbiology.organism_classificationGenome AnnouncementsType speciesMethylocystisOxygenasesbiology.proteinMethylotrophMethylocystis parvusMethaneMethylocystaceaeGenome BacterialMetabolic Networks and PathwaysJournal of Bacteriology
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Isolation and characterisation of an isoproturon-mineralisingMethylopilasp. TES from French agricultural soil

2004

Using enrichment culture three isoproturon (IPU) mineralising bacterial isolates were isolated from a French agricultural soil mineralising up to 50% of the initially added 14C-ring labelled IPU within only eight days. These isolates showed similar metabolic (BIOLOG GN) and amplified rDNA restriction (ARDRA) profiles. Partial 16S rDNA sequencing revealed that they were identical and identified as Methylopila sp TES. This strain harbours a large plasmid (220 kb) putatively bearing essential IPU-degrading genes as demonstrated by a curing experiment. Methylopila sp. TES transformed IPU and its known metabolites to CO2 and biomass but did not degrade chlorotoluron, monolinuron, diuron and linu…

Molecular Sequence DataBiologyDNA RibosomalMicrobiologyEnrichment cultureMicrobiologychemistry.chemical_compoundPlasmidRNA Ribosomal 16SMethylopila sp. TESBotanyGeneticsMolecular BiologySoil MicrobiologyStrain (chemistry)HerbicidesPhenylurea CompoundsAgricultureSequence Analysis DNAMonolinuronBiodegradation16S ribosomal RNAIsolation (microbiology)Bacterial Typing TechniquesCulture MediaBiodegradation EnvironmentalchemistryFranceMethylocystaceaeFEMS Microbiology Letters
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