Search results for "MicroRNA"

Aging and microRNA messaging : associations with systemic estrogen levels and physical performance

2018

This thesis took an innovative approach, which involved methodological development, to investigate microRNAs as candidate molecules and as genome-wide regulators of age, menopausal status and physical condition in both sexes. The focus was on potential serum microRNAs indicative of age, pre- or postmenopausal status, with or without estrogen- based hormone replacement therapy (HRT), and long-term physical training. MicroRNA associations with adiposity and inflammatory markers were also analyzed. Three different study designs were used: 1) the SAWEs study comprised healthy postmenopausal monozygotic twin sisters (n=11, 54-62 years) with discordance for HRT and premenopausal women (n=30, 20-4…

Impact of the flame retardant 2,2'4,4'-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

2023

2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) is one of the most widespread environmental brominated flame-retardant congeners which has also been detected in animal and human tissues. Several studies have reported the effects of PBDEs on different health issues, including neurobehavioral and developmental disorders, reproductive health, and alterations of thyroid function. Much less is known about its immunotoxicity. The aim of our study was to investigate the effects that treatment of THP-1 macrophage-like cells with PBDE-47 could have on the content of small extracellular vesicles’ (sEVs) microRNA (miRNA) cargo and their downstream effects on bystander macrophages. To achieve this, we puri…

Correlación entre la expresión de microRNAs en hipertensión ocular versus glaucoma primario de ángulo abierto

2018

El Glaucoma Primario de Ángulo Abierto (GPAA), la primera causa mundial de ceguera irreversible, aumenta su prevalencia en mayores de 40 años. El primer factor de riesgo es la hipertensión ocular (HTO). No existe un biomarcador que pueda identificar sujetos con mayor predisposición hacia el GPAA. Planteamos la búsqueda de biomarcadores moleculares que ayuden a predecir el riesgo de padecer la enfermedad o de progresión de la misma. Las pequeñas moléculas de ARN no codificante (21-30 nucleótidos) que regulan la expresión génica a nivel posttranscripcional, se denominan miRNAs y se han relacionado con diversas patologías oculares. Esta Tesis Doctoral propone relacionar el perfil diferencial d…

The Administration of Escherichia coli Nissle 1917 Ameliorates Development of DSS-Induced Colitis in Mice

2018

The beneficial effects of probiotics on immune-based pathologies such as inflammatory bowel disease (IBD) have been well reported. However, their exact mechanisms have not been fully elucidated. Few studies have focused on the impact of probiotics on the composition of the colonic microbiota. The aim of the present study was to correlate the intestinal anti-inflammatory activity of the probiotic Escherichia coli Nissle 1917 (EcN) in the dextran sodium sulfate (DSS) model of mouse colitis with the changes induced in colonic microbiota populations. EcN prevented the DSSinduced colonic damage, as evidenced by lower disease activity index (DAI) values and colonic weight/length ratio, when compa…

Cardiorenal Syndrome: New Pathways and Novel Biomarkers

2021

Cardiorenal syndrome (CRS) is a multi-organ disease characterized by the complex interaction between heart and kidney during acute or chronic injury. The pathogenesis of CRS involves metabolic, hemodynamic, neurohormonal, and inflammatory mechanisms, and atherosclerotic degeneration. In the process of better understanding the bi-directional pathophysiological aspects of CRS, the need to find precise and easy-to-use markers has also evolved. Based on the new pathophysiological standpoints and an overall vision of the CRS, the literature on renal, cardiac, metabolic, oxidative, and vascular circulating biomarkers was evaluated. Though the effectiveness of different extensively applied biomark…

Resveratrol, MicroRNAs, Inflammation, and Cancer

2011

MicroRNAs are short noncoding RNAs that regulate the expression of many target genes posttranscriptionally and are thus implicated in a wide array of cellular and developmental processes. The expression ofmiR-155ormiR-21is upregulated during the course of the inflammatory response, but these microRNAs are also considered oncogenes due to their upregulation of expression in several types of tumors. Furthermore, it is now well established that inflammation is associated with the induction or the aggravation of nearly 25% of cancers. Therefore, the above microRNAs are thought to link inflammation and cancer. Recently, resveratrol (trans-3,4′,5-trihydroxystilbene), a natural polyphenol with ant…

Role of RNA Motifs in RNA Interaction with Membrane Lipid Rafts: Implications for Therapeutic Applications of Exosomal RNAs

2021

RNA motifs may promote interactions with exosomes (EXO-motifs) and lipid rafts (RAFT-motifs) that are enriched in exosomal membranes. These interactions can promote selective RNA loading into exosomes. We quantified the affinity between RNA aptamers containing various EXO- and RAFT-motifs and membrane lipid rafts in a liposome model of exosomes by determining the dissociation constants. Analysis of the secondary structure of RNA molecules provided data about the possible location of EXO- and RAFT-motifs within the RNA structure. The affinity of RNAs containing RAFT-motifs (UUGU, UCCC, CUCC, CCCU) and some EXO-motifs (CCCU, UCCU) to rafted liposomes is higher in comparison to aptamers withou…

Binding of RNA Aptamers to Membrane Lipid Rafts: Implications for Exosomal miRNAs Transfer from Cancer to Immune Cells

2020

Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RNA and the raft-like region of the MVB membrane, or through interaction between an RBP&ndash

LIQUID BIOPSY AS A DYNAMIC TOOL FOR LUNG CANCER MANAGEMENT: A FEASIBILITY STUDY FOR PLASMA-DERIVED EXOSOMAL miRNAs AND CELL-FREE DNA.

Estudio de la expresión de MYBL2 y de miRNAs en la leucemia mieloblástica aguda. Papel del miR-30a en la regulación de MYBL2 y en la proliferación ce…

2015

La leucemia mieloblástica aguda (LMA) es una enfermedad heterogénea caracterizada por la transformación neoplásica de las células progenitoras hematopoyéticas. Los blastos leucémicos se acumulan en sangre periférica y en médula ósea, desplazando a las células sanguíneas normales y provocando insuficiencia medular, que se acompaña de infecciones, anemia y desórdenes de la coagulación. Los blastos leucémicos pueden presentar alteraciones genéticas y epigenéticas que, además de explicar la aparición del fenotipo leucémico, pueden ser utilizadas como marcadores pronóstico y diagnóstico de la LMA. MYBL2 es un factor de transcripción que se expresa de forma ubicua en células en proliferación. Su …