Search results for "Microbio"
showing 10 items of 8741 documents
Selection of Lactobacillus strains to induce biological acidification in low acidity wines
2016
Abstract Because of global warming, wines are obtained nowadays with high pH values and low acidity. This results in wines with disturbed flavor and increased susceptibility of microbial spoilage. The aim of this work was the selection of Lactobacillus strains with ability to induce biological acidification in low acidity grape musts to obtain more acidic wines. A screening of Lactobacillus strains was carried out using several selection criteria. Lactobacillus strains that grew in must, carried out the malolactic fermentation, acidified grape must, synthesized lactic acid from sugars, and showed high resistance to lysozyme and sulfur dioxide were selected. Selected strains were characteriz…
Shimia thalassica sp. nov., and reclassification of Pseudopelagicola gijangensis as Shimia gijangensis comb. nov., and Thalassobius activus as Cognat…
2019
Strain CECT 7735T, a marine Gram-reaction negative, aerobic, non-motile bacterium, was isolated from coastal seawater in Valencia, Spain. Strain CECT 7735T is chemoorganotrophic, mesophilic, slightly halophilic, grows at 15–28 °C but not at 4 or 37 °C, requires seawater for growth and grows up to 6 % salinity. The major cellular fatty acid is summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The G+C content of the genome is 55.7 mol%. Comparative analysis of the 16S rRNA gene sequence shows the strain is affiliated to the family Rhodobacteraceae , in the class Alphaproteobacteria , with highest similarities to Phaeobacter species (97.0–97.5 %), Shimia species (96.5–97.3 %) and Pseudopelagico…
Preparation of Samples for Characterization of Arbuscular Mycorrhizal Fungi
2020
Arbuscular mycorrhizal fungi (AMF) are an important element of the plant microbiome as they establish an endosymbiotic relationship with the roots of most plant species. This association enhances access to nutrients and water for plants, and provides the fungus with plant-derived organic carbon. In this chapter, I describe a range of methods to work with AMF including: soil sampling; isolation of AMF propagules (spores, sporocarps, roots, and mycelium) by a wet sieving and centrifugation in a sucrose solution; trap (from field soil with AMF spores) and one-species pot cultures (from AMF spores divided into morphotypes); staining of mycorrhizae in plant roots; and production of diagnostic sl…
In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum
2017
Abstract Food spoilage caused by mycotoxigenic moulds represents an important problem in food security. The antimicrobial peptides are compounds of natural origin constituted by a variable number (5–100) of amino acids held together through peptide bonds. In this work, the cell free supernatants (CFSs) containing peptides obtained from four strains of LAB were lyophilized, filtered and tested to determine the antifungal activity against Aspergillus Parasiticus and Penicillium expansum . CFS obtained by Lactobacillus plantarum showed the highest inhibition activity. CFS was fractionated by size exclusion chromatography and injected into the liquid chromatography coupled to diode array detect…
Importance of Endophytic Strains Pantoea agglomerans in the Biological Control of Rhizoctonia solani
2018
Abstract Antagonistic activity of microorganisms against phytopathogens is mainly the results of plants’ health improvement due to the inhibition of pathogens growth and the induction of plants resistance against diseases. The aim of the research was to determine antagonistic properties of Pantoea agglomerans against Rhizoctonia solani. The properties of two strains P. agglomerans BC17 and BC45 were assessed according to the following criteria: mycelial growth of R. solani in the presence of bacterial metabolites, an impact of P. agglomerans on the growth of sugar beet in the pots containing soil with and addition of R. solani and without it, the ability to produce indole-3-acetic acid (IAA…
Characterization of a β-glucosidase isolated from an alpeorujo strain of Candida adriatica
2017
ABSTRACTA β-glucosidase-producing strain, Candida adriatica CECT13142, was isolated from olive oil wastes (alpeorujo) and identified by PCR/restriction fragment length polymorphism of the rDNA internal transcribed spacer and sequence analysis of the D1/D2 region of the 26S rRNA gene techniques. The enzyme was purified by sequential chromatography on DEAE-cellulose and Sephadex G-100. The relative molecular mass of the enzyme was estimated to be 50 kDa by SDS-PAGE. The hydrolytic activity of the β-glucosidase had an optimum pH of 8.2 and an optimum temperature of 40°C. The enzyme displayed high substrate specificity and high catalytic efficiency (Km 0.85 mM, Vmax 12.5 U/g of cells) for p-nit…
The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants
2021
α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,β-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal <
Soil microbiome of primeval forest ecosystems in Transkarpathia
2018
The aim of this study was to investigate the soil microbiome of primeval forest ecosystems, namely the structure of microbial communities,the number of major ecological-functional groups, functional parameters such as: soil toxicity, as well as enzymatic activity of the soil by the level of catalase and invertase. To analyze the successional processes in the soil microbiocenosis due to the influence of endogenous and exogenous factors. To estimate the integrity of microbial communities in different edaphotopes of primeval forest ecosystems. Methods. Microbiological studies of soil were carried out according to generally accepted methods in soil microbiology. Enzymatic activity of the soil: …
Sperm Quality Assessment in Honey Bee Drones
2020
The quality of honey bee drone semen is relevant in different contexts, ranging from colony productivity to pathology, toxicology and biodiversity preservation. Despite its importance, considerably less knowledge is available on this subject for the honey bee when compared to other domestic animal species. A proper assessment of sperm quality requires a multiple testing approach which discriminates between the different aspects of sperm integrity and functionality. Most studies on drone semen quality have only assessed a few parameters, such as sperm volume, sperm concentration and/or sperm plasma membrane integrity. Although more recent studies have focused on a broader variety of aspects …
Tubercle disease (Xanthomonas beticola) and other gall-malformed diseases of sugar beet roots: a review
2016
The sugar beet (Beta vulgaris) is an important plant in agriculture and sugar industry, and it is widely cultivated in European countries. Getting proper raw material of sugar beets (roots) is a problem for agriculture. Some disease symptoms observed on sugar beet roots are atypical tumor-like deformations. The causative agent of these deformations is known in the old literature as Xanthomonas beticola. The disease’s name in Poland is “tuberkuloza” and in the USA it refers to a description of a pocket disease—therefore we may consider those diseases to be the same. The clear description of X. beticola disease can be found in many phytopathological manuals printed in the past and nowadays. S…