Search results for "Microbio"

The evolution, diversity and host associations of rhabdoviruses

2015

Metagenomic studies are leading to the discovery of a hidden diversity of RNA viruses, but new approaches are needed predict the host species these poorly characterised viruses pose a risk to. The rhabdoviruses are a diverse family of RNA viruses that includes important pathogens of humans, animals and plants. We have discovered the sequences of 32 new rhabdoviruses through a combination of our own RNA sequencing of insects and searching public sequence databases. Combining these with previously known sequences we reconstructed the phylogeny of 195 rhabdovirus sequences producing the most in depth analysis of the family to date. In most cases we know nothing about the biology of the viruses…

Occurrence of antibiotics and antiretroviral drugs in source-separated urine, groundwater, surface water and wastewater in the peri-urban area of Chu…

2020

Recently, there has been an increased interest in bridging the knowledge gap in the occurrence and fate of pharmaceuticals in African urban water  cycles. In this study, the occurrence of 7 antibiotics and 3 antiretrovirals in source-separated urine, groundwater, wastewater and surface water of the peri-urban area of Chunga in Lusaka, Zambia, was studied. In groundwater, the pharmaceuticals were only sporadically present with 4 antibiotics and 1 antiretroviral detected. The concentration of the antibiotics ranged from below limit of quantification (<LOQ) to 880 ng/L, with sulfamethoxazole having the highest detection frequency of 42.3%. In the surface water, a comparatively high concentrati…

Beyond cells – The virome in the human holobiont

2019

Viromics, or viral metagenomics, is a relatively new and burgeoning field of research that studies the complete collection of viruses forming part of the microbiota in any given niche. It has strong foundations rooted in over a century of discoveries in the field of virology and recent advances in molecular biology and sequencing technologies. Historically, most studies have deconstructed the concept of viruses into a simplified perception of viral agents as mere pathogens, which demerits the scope of large-scale viromic analyses. Viruses are, in fact, much more than regular parasites. They are by far the most dynamic and abundant entity and the greatest killers on the planet, as well as th…

Performance and feasibility of biotrickling filtration in the control of styrene industrial air emissions

2017

Abstract The performance and feasibility of a pilot unit of biotrickling filter (BTF) for the treatment of industrial emissions polluted by styrene was investigated for one year at a fiber reinforced plastic industrial site. The pilot unit was packed with a structured material with a volume of 0.6 m3. Monitoring results have shown successful treatment of the industrial styrene emissions working at empty bed residence times (EBRT) between 31 and 66 s. The best performance was obtained after 300 days when a more stable biofilm had been developed, obtaining the highest elimination capacity of 18.8 g m−3 h−1 (removal efficiency of 75.6%) working at 31 s of EBRT. In addition, a photocatalytic re…

2020

ABSTRACT The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajärvi and Lake Lovojärvi. Furthermore, metagenomic analyses were performed to assess the genomic characteristics of methanotrophs in Lovojärvi and the previously studied Lake Alinen Mustajärvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative…

'Palaeoshellomics' reveals the use of freshwater mother-of-pearl in prehistory

2019

The extensive use of mollusc shell as a versatile raw material is testament to its importance in prehistoric times. The consistent choice of certain species for different purposes, including the making of ornaments, is a direct representation of how humans viewed and exploited their environment. The necessary taxonomic information, however, is often impossible to obtain from objects that are small, heavily worked or degraded. Here we propose a novel biogeochemical approach to track the biological origin of prehistoric mollusc shell. We conducted an in-depth study of archaeological ornaments using microstructural, geochemical and biomolecular analyses, including ‘palaeoshellomics’, the first…

Happens in the best of subfamilies: establishment and repeated replacements of co-obligate secondary endosymbionts within Lachninae aphids.

2016

SummaryVirtually all aphids maintain an obligate mutualistic symbiosis with bacteria from theBuchneragenus, which produce essential nutrients for their aphid hosts. Most aphids from the Lachninae subfamily have been consistently found to house additional endosymbionts, mainlySerratia symbiotica. This apparent dependence on secondary endosymbionts was proposed to have been triggered by the loss of the riboflavin biosynthetic capability byBuchnerain the Lachninae last common ancestor. However, an integral large-scale analysis of secondary endosymbionts in the Lachninae is still missing, hampering the interpretation of the evolutionary and genomic analyses of these endosymbionts. Here, we anal…

The Comparison of Cellulolytic Activity of the Modified Soil Treated with Roundup

2016

Abstract The activity of soil microorganisms affects soil fertility and structure, what leads to satisfactory crop yields, thanks to changes in the decay of organic matter. Their activity may be impaired as a result of application by farmers both fertilizers and pesticides. The degradation of cellulosic biomass represents an important part of the carbon cycle within the biosphere. Cellulolitic microorganisms are responsible for this decomposition, as they produced the enzymes of the cellulase complex. The aim of the study was to compare the cellulolytic activity of soil with Roundup and modified using urea phosphate and / or manure. The pot experiment was conducted under laboratory conditio…

Pathogenic Escherichia coli Among Asymptomatic Children and Associated Factors

2020

Abstract Although Escherichia coli is a part of the commensal flora of the gastrointestinal tract, pathogenic types of E. coli can cause diarrhea, especially in children. Pathogenic types are found also in healthy individuals, but prevalence of pathogenic E. coli among asymptomatic children varies and has not been studied in Latvia. The aim of the study was to determine prevalence of pathogenic E. coli among asymptomatic children and identify factors associated with presence of bacterium. Children (aged 0.5–8 years) without acute gastrointestinal symptoms were included in a cross-sectional study. Parents were asked to answer a questionnaire (demographic data, parental education, type of del…

La couverture vaccinale contre la grippe saisonnière du personnel soignant en gériatrie : mise au point

2016

Resume Position du probleme La vaccination antigrippale des professionnels de sante semble representer l’une des actions preventives des plus efficaces face a une pathologie a haut risque d’epidemie nosocomiale et potentiellement grave en milieu geriatrique. Ce travail avait pour objectif de faire une mise au point sur le statut vaccinal antigrippal chez le personnel soignant en geriatrie et les raisons expliquant leur reticence vis-a-vis de cette vaccination, afin d’emettre des propositions d’amelioration de la couverture vaccinale. Methode Une recherche bibliographique des articles publies depuis 2000 dans le domaine de la geriatrie, des maladies infectieuses ou de la pneumologie a ete re…