Search results for "Microorganisms"
showing 10 items of 78 documents
Use of rumen microorganisms to boost the anaerobic biodegradability of microalgae
2017
[EN] A laboratory bioreactor using rumen microorganisms to treat Scenedesmus spp. biomass was operated for 190 days. At first the bioreactor operated as a Rumen-like Fermenter (RF) with a Sludge Retention Time (SRT) of 7 days. The RF was subsequently transformed into an anaerobic digestion system including two configurations: continuously-stirred tank reactor and anaerobic membrane bioreactor in which different SRT values of up to 100 days were assessed. Methane production peaked at 214 mL CH4 g−1 CODIn with a SRT of 100 days. COD removal and BDP peaked at above 70% and 60%, respectively, at the highest SRT, with no pre-treatment prior to microalgae digestion. The waste sludge product…
Evolution of microbiological and chemical parameters during red wine making with extended post-fermentation maceration.
2014
Abstract The aim of the present work was to investigate the microbiological, chemical, and sensory characteristics of red wine subjected to post-fermentation maceration that was extended to 90 days. For this purpose, the ‘Aglianico di Taurasi’ grape was used as a case study. The total yeast concentration increased until day 40 of maceration and decreased thereafter, whereas the concentration of lactic acid bacteria slightly increased. Dekkera/Brettanomyces spp. and acetic acid bacteria were not detected. The yeast community was composed of Saccharomyces cerevisiae, Zygosaccharomyces bisporus, Metschnikowia pulcherrima, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Pichia guilliermondi…
Application of model quality evaluation to systems biology
2008
Application of model quality evaluation to the quasispecies models is presented. These models are useful for the analysis of the DNA and RNA evolution and for the description of the population dynamics of viruses and bacteria. An estimate of the parameters together with their interval of variability is computed and the quality evaluation is tested on the basis of the model prediction error capability.
Contribution of Molecular Structure to Self-Assembling and Biological Properties of Bifunctional Lipid-Like 4-(N-Alkylpyridinium)-1,4-Dihydropyridines
2019
The design of nanoparticle delivery materials possessing biological activities is an attractive strategy for the development of various therapies. In this study, 11 cationic amphiphilic 4-(N-alkylpyridinium)-1,4-dihydropyridine (1,4-DHP) derivatives differing in alkyl chain length and propargyl moiety/ties number and position were selected for the study of their self-assembling properties, evaluation of their cytotoxicity in vitro and toxicity on microorganisms, and the characterisation of their interaction with phospholipids. These lipid-like 1,4-DHPs have been earlier proposed as promising nanocarriers for DNA delivery. We have revealed that the mean diameter of freshly prepared nanoparti…
Comparison of different primer sets for use in Automated Ribosomal Intergenic Spacer Analysis of complex bacterial communities.
2004
ABSTRACT ITSF and ITSReub, constituting a new primer set designed for the amplification of the 16S-23S rRNA intergenic transcribed spacers, have been compared with primer sets consisting of 1406F and 23Sr (M. M. Fisher and E. W. Triplett, Appl. Environ. Microbiol. 65:4630-4636, 1999) and S-D-Bact-1522-b-S-20 and L-D-Bact-132-a-A-18 (L. Ranjard et al., Appl. Environ. Microbiol. 67:4479-4487, 2001), previously proposed for automated ribosomal intergenic spacer analysis (ARISA) of complex bacterial communities. An agricultural soil and a polluted soil, maize silage, goat milk, a small marble sample from the façade of the Certosa of Pavia (Pavia, Italy), and brine from a deep hypersaline anoxi…
Ocena aktywności przeciwdrobnoustrojowej ekstraktu roślinnego z Dionaea muscipula
2018
Rośliny owadożerne są przedmiotem badań, nie tylko ze względu na charakterystyczny dla nich sposób pobierania składników odżywczych z otoczenia, ale również z powodu ich pożądanego oddziaływania na mikroorganizmy. W piśmiennictwie znajdujemy potwierdzenie, że ekstrakty z roślin owadożernych mają działanie nie tylko bakteriostatyczne, ale również bakteriobójcze i grzybobójcze. Do badań wybrano jednego z przedstawicieli roślin owadożernych, Dionaea muscipula J. Ellis (Muchołówka amerykańska), w celu określenia ich aktywności biologicznej. Materiałem do badań były odpowiednio przygotowanie ekstrakty z dwóch odmian Dionaea muscipula: Regular Form oraz Red Green. Hodowle prowadzono w warunkach ś…
Cultivable microorganisms associated with honeys of different geographical and botanical origin
2014
In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-IT…
Offspring microbiomes differ across breeding sites in a panmictic species.
2019
High dispersal rates are known to homogenize host’s population genetic structure in panmictic species and to disrupt host local adaptation to the environment. Long-distance dispersal might also spread micro-organisms across large geographical areas. However, so far, to which extent selection mechanisms that shape host’s population genetics are mirrored in the population structure of the enteric microbiome remains unclear. High dispersal rates and horizontal parental transfer may homogenize bacterial communities between breeding sites (homogeneous hypothesis). Alternatively, strong selection from the local environment may differentiate bacterial communities between breeding sites (heterogene…
Competitive interactions in insect parasitoids: effects of microbial symbionts across tritrophic levels
2023
Competition for hosts is a common ecological interaction in insect parasitoids. In the recent years, it has become increasingly evident that microorganisms can act as ‘hidden players’ in parasitoid ecology. In this review, we propose that parasitoid competition should take into consideration the microbial influence. In particular, we take a tritrophic perspective and discuss how parasitoid competition can be modulated by microorganisms associated with the parasitoids, their herbivore hosts, or the plants attacked by the herbivores. Although research is still in its infancy, recent studies have shown that microbial symbionts can modulate the contest outcome. The emerging pattern is that micr…
Herbicidal activity of Mediterranean essential oils and their effects on soil bioindicators.
2020
[ES] Las preocupaciones ambientales y de salud han estimulado el interés en estrategias alternativas para el manejo de las malas hierbas. En todo el mundo se están haciendo esfuerzos para reducir la gran dependencia de los herbicidas sintéticos que se utilizan como principal método para el control de las plantas arvenses. Los herbicidas naturales basados en sustancias alelopáticas, como los aceites esenciales (AEs) extraídos de plantas, se han sugerido como una de las posibles alternativas para lograr un manejo sostenible de las arvenses. Por un lado, los AEs han mostrado capacidad para inhibir la germinación y el crecimiento de semillas de malas hierbas, por otro lado, hay una falta de est…