Search results for "Micropropagation"

Micropropagation of Citrus spp. by Organogenesis and Somatic Embryogenesis.

2013

Citrus spp., the largest fruit crops produced worldwide, are usually asexually propagated by cuttings or grafting onto seedling rootstocks. Most of Citrus genotypes are characterized by polyembryony due to the occurrence of adventive nucellar embryos, which lead to the production of true-to-type plants by seed germination. Tissue culture and micropropagation, in particular, are valuable alternatives to traditional propagation to obtain a high number of uniform and healthy plants in a short time and in a small space. Moreover, in vitro propagation provides a rapid system to multiply the progeny obtained by breeding programs, allows the use of monoembryonic and seedless genotypes as rootstock…

Propagation techniques for Iberis semperflorens L.

2009

Micropropagation ofAgeratum houstonianumby nodal segments

2017

Ageratum houstonianum is a bedding and flowering potted plant originated from Central America which is generally propagate by seed. In this report a preliminary in vitro technique for propagation of A. houstonianum was investigated. In vitro germinated seeds were used to establish aseptic shoot cultures of several clones. Seedling stem segments bearing 3-4 nodes were placed on Murashige and Skoog (MS) basal medium plus 20 g L-1 sucrose, 8.0 g L-1 Agar to induce axillary shoot development. Axillary shoots were subcultured into the same medium and nodal segments were sectioned and subcultured to increase the stock of shoot cultures. Shoot cultures of the selected clone AG14 were used to accom…

Produzione di semi sintetici di Capparis spinosa (L.). Innovazione e tradizione

2011

The caper bush (Capparis spinosa L.) is a rupiculous species. It is widespread on rocky areas and it grows on different soil associations. Caper can be propagated from fresh seeds, gathered from ripe fruit, or by stem cuttings both methods with unsatisfying results, due to the low germination percentage and to the season-dependent answer of the cutting technique. The aim of the present research has been to evaluate the attitude, for caper in vitro propagules , to be encapsulated. Micro cuttings from in vitro plantlets, were dissected and put on two different Murashige and Skoog liquid media enriched with 4% sodium alginate and 1,4% di CaCl2 separately, for the encapsulation. The synthetic s…

Multiplication of Crataegus monogyna by in vitro culture of nodal segments

2009

Recovering Genetic Resources of Some Legume Species of Latvian Origin by Plant Tissue Culture

2013

Accessions with no germinating seeds are a common problem in plant gene banks and research institutions. Our goal was to elaborate and apply an in vitro method of germination and multiplication of old aged seeds of red and alsike clover and alfalfa. Eighteen clover and five alfalfa accessions were used for germination in vitro. Most of the accessions had produced seeds more than 20 years ago and the seeds did not germinate in soil. Seed pre-treatment with different concentrations of potassium permanganate, as well as addition of phytohormones, AgNO3 and activated carbon to germinating media were tested. Plantlets for all germinated accessions were obtained, even in the case when seeds were …

In vitro multiplication ofVella lucentina M. B. crespo (Brassicaceae), a threatened Spanish endemic species

1995

Vella lucentina M. B. Crespo is a threatened Spanish species that is endemic to a small area in eastern Alicante Province (SE Spain). Micropropagation techniques were applied forex situ conservation of this plant. Aseptic epicotyls bearing the apical bud were grown in Murashige and Skoog medium supplemented with 6-furfurylaminopurine (Kin), N6-benzyladenine (BA) or 6-(γ,γ,-dimethilalylamino) purine (2iP). High multiplication rates were obtained with 0.5, 1, or 2 mg·liter−1 BA, or 1 or 2 mg·liter−1 2iP. Indole-3-acetic acid and indole-3-butyric acid were utilized for rooting in half-strength Murashige and Skoog medium. Regenerated plants were transferred to a potting mix and gradually acclim…

Factors affectingin vitro shoot proliferation ofMyrtus communis L.: A comparison of adult and seedling material

1998

Two stocks of shoots growingin vitro, obtained from either seedlings or adult plants, were used to study the effects of material origin, the number of previous subcultures on the establishment medium, the explant type, and the macronutrients on shoot multiplication and elongation inMyrtus communis L., always in the presence of 4.4. μM benzyladenine (BA). Shoot proliferation was influenced mainly by stock origin, with higher responses from the adult material than from the seedling material, and by the number of subcultures, with the largest rates of multiplication and elongation in the first subculture. In the first subculture, the adult material was characterized by high rates of shoot mult…

In vitro Shoot Regeneration from Flower and Leaf Explants in Rhododendron

2003

Rhododendron shoot regeneration was accomplished using either flower explants (each consisting of ovary with pedicel) of Rhododendron cvs. Nova Zembla and Irina or leaves isolated from in vitro grown Rhododendron catawbiense Michx. Multiple shoot tip clumps were obtained on Anderson's medium containing 0.5 to 1.5 mg dm−3 thidiazuron (TDZ) in combination with 12 to 15 mg dm−3 N6-[2-isopentenyl]adenine (2iP) and 1 to 3 mg dm−3 indole-3-butyric acid (IBA). After 16 weeks on the regeneration media, explants with shoot tip clumps were transferred for shoot elongation to Anderson's medium with 3 mg dm−3 2iP. Two months later, the shoots have reached 5 to 40 mm in length and were fit for subcultiv…

Foreed flushing of branch segments as a method for obtaining reactive explants of mature Quercus robur trees for micropropagation

1994

The aim of this study was to micropropagate mature Quercus robur L. trees when material retaining physiologically juvenile characteristics (stump sprouts, epicormic shoots) is not available. Branch segments from 70–300 year-old trees were force-flushed and the flushed, partially rejuvenated or reinvigorated shoots were used as a source of explants for establishment of cultures. In vitro establishment and multiplication was achieved with seven of the eight selected trees. The proliferation capacity of cultures of vertically placed explants declined after several subcultures, but efficient shoot multiplication was achieved by culturing decapitated shoots placed horizontally on GD medium suppl…