Search results for "Microreview"

showing 3 items of 3 documents

Mutant p53 induces Golgi tubulo-vesiculation driving a prometastatic secretome

2020

TP53 missense mutations leading to the expression of mutant p53 oncoproteins are frequent driver events during tumorigenesis. p53 mutants promote tumor growth, metastasis and chemoresistance by affecting fundamental cellular pathways and functions. Here, we demonstrate that p53 mutants modify structure and function of the Golgi apparatus, culminating in the increased release of a pro-malignant secretome by tumor cells and primary fibroblasts from patients with Li-Fraumeni cancer predisposition syndrome. Mechanistically, interacting with the hypoxia responsive factor HIF1α, mutant p53 induces the expression of miR-30d, which in turn causes tubulo-vesiculation of the Golgi apparatus, leading …

0301 basic medicineBiopsyGeneral Physics and AstronomyGolgi ApparatusAnimals Biopsy Breast Neoplasms Cell Line Tumor Cell Transformation Neoplastic Female Fibroblasts Gene Expression Regulation Neoplastic Golgi Apparatus Humans Hypoxia-Inducible Factor 1 alpha Subunit Li-Fraumeni Syndrome Mice MicroRNAs Microtubules Mutation Primary Cell Culture Secretory Vesicles Signal TransductionSkin Tumor Microenvironment Tumor Suppressor Protein p53 Xenograft Model Antitumor Assays02 engineering and technologymedicine.disease_causeCell TransformationMicrotubulesSettore BIO/09 - FisiologiaMetastasisLi-Fraumeni SyndromeMiceTumor MicroenvironmentGolgisecretory machinerySuper-resolution microscopyAnimals; Biopsy; Breast Neoplasms; Cell Line Tumor; Cell Transformation Neoplastic; Female; Fibroblasts; Gene Expression Regulation Neoplastic; Golgi Apparatus; Humans; Hypoxia-Inducible Factor 1 alpha Subunit; Li-Fraumeni Syndrome; Mice; MicroRNAs; Microtubules; Mutation; Primary Cell Culture; Secretory Vesicles; Signal Transduction; Skin; Tumor Microenvironment; Tumor Suppressor Protein p53; Xenograft Model Antitumor Assayslcsh:ScienceSkinMultidisciplinaryTumorChemistrymutant p53QCell migrationMicroRNASecretomics021001 nanoscience & nanotechnologyCell biologyGene Expression Regulation NeoplasticCell Transformation NeoplasticsymbolsFibroblastmiR-30dFemaleHypoxia-Inducible Factor 10210 nano-technologyBreast NeoplasmHumanSignal TransductionCancer microenvironmentStromal cellSecretory VesicleSciencePrimary Cell CultureBreast NeoplasmsMicrotubuleGolgi ApparatuSettore MED/08 - Anatomia Patologicaalpha SubunitGeneral Biochemistry Genetics and Molecular BiologyArticleCell Line03 medical and health sciencessymbols.namesakeCell Line TumormedicineAnimalsHumansSettore MED/05 - Patologia ClinicaSecretionTumor microenvironmentNeoplasticAnimalSecretory VesiclesGeneral ChemistryOncogenesGolgi apparatusHDAC6FibroblastsMicroreviewHypoxia-Inducible Factor 1 alpha SubunitmicroenvironmentXenograft Model Antitumor AssaysMicroRNAs030104 developmental biologyGene Expression RegulationMutationlcsh:QTumor Suppressor Protein p53Carcinogenesis
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The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice

2019

Our results demonstrate a functional role for the commensal microbiota in atherothrombosis. In a ferric chloride injury model of the carotid artery, GF C57BL/6J mice had increased occlusion times compared to colonized controls. Interestingly, in late atherosclerosis, HFD-fed GF Ldlr−/− mice had reduced plaque rupture-induced thrombus growth in the carotid artery and diminished ex vivo thrombus formation under arterial flow conditions.

Male0209 industrial biotechnologyVery low-density lipoproteinChemokine CXCL102 engineering and technology030204 cardiovascular system & hematologyarterial thrombosisApplied Microbiology and BiotechnologyACTIVATIONMicechemistry.chemical_compound020901 industrial engineering & automation0302 clinical medicinegermfree0202 electrical engineering electronic engineering information engineeringMedicinevascular inflammationPlateletChemokine CCL7lcsh:QH301-705.5platelet0303 health sciencesatherosclerosis mouse modelsfood and beveragesThrombosisPlaque AtheroscleroticQR1-502late atherosclerosis3. Good healthHolobiontlow-density lipoprotein receptorgerm-freeplateletscardiovascular systemFemalelipids (amino acids peptides and proteins)GLYCOPROTEIN-VIBlood streamResearch ArticleRECRUITMENTmedicine.medical_specialtyNutritional compositionCOAGULATION610 Medicine & healthBiologyMETABOLISMBiochemistry Genetics and Molecular Biology (miscellaneous)MicrobiologyMicrobiologyHost-Microbe BiologyProinflammatory cytokinePLATELET HYPERREACTIVITY03 medical and health sciencesINFLAMMATIONVirologyInternal medicineatherothrombosisGeneticsmicrobiotaAnimalsInterleukin 9Platelet activationcardiovascular diseasesThrombusMolecular Biology030304 developmental biologygut microbiotabusiness.industryCholesterolcarotid artery020208 electrical & electronic engineeringcholesterolnutritional and metabolic diseasesCell Biologymedicine.diseaseMicroreviewCHLAMYDIA-PNEUMONIAEMice Mutant StrainsGastrointestinal MicrobiomeEndocrinologyReceptors LDLlcsh:Biology (General)chemistryArterial thrombusLDL receptorParasitologyatherosclerosisbusinessEx vivoLipoproteinmBio
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Small RNA‐binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli

2019

Abstract The RNA‐binding protein RapZ cooperates with small RNAs (sRNAs) GlmY and GlmZ to regulate the glmS mRNA in Escherichia coli. Enzyme GlmS synthesizes glucosamine‐6‐phosphate (GlcN6P), initiating cell envelope biosynthesis. GlmZ activates glmS expression by base‐pairing. When GlcN6P is ample, GlmZ is bound by RapZ and degraded through ribonuclease recruitment. Upon GlcN6P depletion, the decoy sRNA GlmY accumulates through a previously unknown mechanism and sequesters RapZ, suppressing GlmZ decay. This circuit ensures GlcN6P homeostasis and thereby envelope integrity. In this work, we identify RapZ as GlcN6P receptor. GlcN6P‐free RapZ stimulates phosphorylation of the two‐component sy…

Small RNAsmall regulatory RNAcell envelope precursor glucosamine‐6‐phosphatemedicine.disease_causenegative feedback loopmetabolite sensing0302 clinical medicinetwo-component system QseE-QseFRNA-binding protein RapZRNA‐binding protein RapZGlucosamine0303 health sciencesbiologyEscherichia coli ProteinsGeneral NeuroscienceRNA-Binding ProteinsArticlesRNA BiologyMicrobiology Virology & Host Pathogen InteractionReceptors AdrenergicCell biologyDNA-Binding ProteinsRNA BacterialTransfer RNAPhosphorylationCell envelopeSignal TransductionGlucose-6-PhosphateGeneral Biochemistry Genetics and Molecular BiologyArticletwo‐component system QseE‐QseF03 medical and health sciencesBacterial Proteinscell envelope precursorEscherichia colimedicineRNA MessengerRibonucleaseMolecular BiologyEscherichia coli030304 developmental biologyMessenger RNAGeneral Immunology and MicrobiologyBinding proteinsmall RNAs GlmY and GlmZGene Expression Regulation BacterialMicroreviewRNA binding proteincell envelope precursor glucosamine-6-phosphatetwo-component systembiology.proteinRNA Small Untranslated030217 neurology & neurosurgeryThe EMBO Journal
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